张金乾,张成,刘自成,施万喜,孟建军,杨虓.陇东旱塬冬小麦资源的HMW-GS组成及品质比较[J].干旱地区农业研究,2016,34(2):146~151 |
陇东旱塬冬小麦资源的HMW-GS组成及品质比较 |
Compositions of HMW-GS and quality properties of winter wheat varieties in Longdong drought area |
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DOI:10.7606/j.issn.1000-7601.2016.02.24 |
中文关键词: 小麦 高分子量麦谷蛋白亚基 育成品种 品质 |
英文关键词:Wheat HMW-GS Bred varieties Quality |
基金项目:高产优质多抗小麦新品种示范与推广(GB2B02011100007) |
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中文摘要: |
采用SDS-PAGE技术和近红外反射光谱法,对陇东地区382份冬小麦资源的HMW-GS组成和其中315份品种的沉淀值、蛋白质和湿面筋含量进行了检测。结果表明:陇东旱塬冬小麦资源存在16种亚基变异类型,35种组合形式;null,7+8,2+12为各自位点的主要亚基变异类型,其中 1,7+9,5+12亚基在育成和引进品种(系)中相对地方品种有明显提高,且5+10亚基在育成品种(系)中出现频率也较高(17.02%);在地方品种中null、7+8、2+12为优势组合(占79.19%),而育成和引进品种(系)中亚基主要以null、7+9、2+12(23.40%和17.58%),null、7+8、2+12(21.28%和16.48%),1、7+8、2+12(10.60%和15.38%)三类组合形式存在;具有2*、7+8、5+12组合的小麦品种综合品质较优。从供试材料中筛选出8分以上的组合14类,89个品种,其中4类组合的23个品种得分10分。 |
英文摘要: |
The compositions of high molecular weight glutenin subunits were investigated through SDS-PAGE analyses using 382 winter wheat varieties from Longdong, and sedimentation value, protein and wet gluten contents of 315 cultivars were deciphered with a near infrared analyzer. The results showed that there were 16 types of subunits and 35 subunit compositions in these materials. Among them, the null, 7+8, and 2+12 subunits were the major types for their respective loci. The subunits of 1, 7+9, and 5+12 were increased obviously in bred and introduced varieties from those in local varieties, and the frequency (17.02%) of subunit 5+10 was high in bred varieties. The subunit composition with null, 7+8, and 2+12 was the major type in local varieties. However, in bred and introduced varieties, subunit compositions with null, 7+9 and 2+12 (23.40% and 17.58% respectively), null, 7+8 and 2+12 (21.28% and 16.48% respectively), and 1, 7+8 and 2+12 (10.60% and 15.38% respectively) were the dominant combinations. Varieties with the composition of 2*, 7+8, and 5+12 had relatively superior quality. According to the subunit combinations, qualities of 89 winter wheat varieties scored above 8, and 23 varieties even had a score of 10 in this experiment. |
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