张金乾,张成,刘自成,施万喜,孟建军,杨虓.陇东旱塬冬小麦资源的HMW-GS组成及品质比较[J].干旱地区农业研究,2016,34(2):146~151
陇东旱塬冬小麦资源的HMW-GS组成及品质比较
Compositions of HMW-GS and quality properties of winter wheat varieties in Longdong drought area
  
DOI:10.7606/j.issn.1000-7601.2016.02.24
中文关键词:  小麦  高分子量麦谷蛋白亚基  育成品种  品质
英文关键词:Wheat  HMW-GS  Bred varieties  Quality
基金项目:高产优质多抗小麦新品种示范与推广(GB2B02011100007)
作者单位
张金乾 甘肃农业大学农学院 甘肃 兰州 730070 
张成 陇东学院农林科技学院 甘肃 庆阳 745000 
刘自成 陇东学院农林科技学院 甘肃 庆阳 745000 
施万喜 陇东学院农林科技学院 甘肃 庆阳 745000 
孟建军 陇东学院农林科技学院 甘肃 庆阳 745000 
杨虓 陇东学院农林科技学院 甘肃 庆阳 745000 
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中文摘要:
      采用SDS-PAGE技术和近红外反射光谱法,对陇东地区382份冬小麦资源的HMW-GS组成和其中315份品种的沉淀值、蛋白质和湿面筋含量进行了检测。结果表明:陇东旱塬冬小麦资源存在16种亚基变异类型,35种组合形式;null,7+8,2+12为各自位点的主要亚基变异类型,其中 1,7+9,5+12亚基在育成和引进品种(系)中相对地方品种有明显提高,且5+10亚基在育成品种(系)中出现频率也较高(17.02%);在地方品种中null、7+8、2+12为优势组合(占79.19%),而育成和引进品种(系)中亚基主要以null、7+9、2+12(23.40%和17.58%),null、7+8、2+12(21.28%和16.48%),1、7+8、2+12(10.60%和15.38%)三类组合形式存在;具有2*、7+8、5+12组合的小麦品种综合品质较优。从供试材料中筛选出8分以上的组合14类,89个品种,其中4类组合的23个品种得分10分。
英文摘要:
      The compositions of high molecular weight glutenin subunits were investigated through SDS-PAGE analyses using 382 winter wheat varieties from Longdong, and sedimentation value, protein and wet gluten contents of 315 cultivars were deciphered with a near infrared analyzer. The results showed that there were 16 types of subunits and 35 subunit compositions in these materials. Among them, the null, 7+8, and 2+12 subunits were the major types for their respective loci. The subunits of 1, 7+9, and 5+12 were increased obviously in bred and introduced varieties from those in local varieties, and the frequency (17.02%) of subunit 5+10 was high in bred varieties. The subunit composition with null, 7+8, and 2+12 was the major type in local varieties. However, in bred and introduced varieties, subunit compositions with null, 7+9 and 2+12 (23.40% and 17.58% respectively), null, 7+8 and 2+12 (21.28% and 16.48% respectively), and 1, 7+8 and 2+12 (10.60% and 15.38% respectively) were the dominant combinations. Varieties with the composition of 2*, 7+8, and 5+12 had relatively superior quality. According to the subunit combinations, qualities of 89 winter wheat varieties scored above 8, and 23 varieties even had a score of 10 in this experiment.
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