| 沈宝云,李朝周,余斌,张俊莲,王蒂,白江平,王文,康小华,王海龙.甘肃沿黄灌区连作马铃薯根区土壤有机物GC-MS分析[J].干旱地区农业研究,2016,34(3):1~7 |
| 甘肃沿黄灌区连作马铃薯根区土壤有机物GC-MS分析 |
| GC-MS analysis of rhizosphere soil organic compounds of potato field under continuous cropping in the Yellow River irrigation region of Gansu Province |
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| DOI：10.7606/j.issn.1000-7601.2016.03.01 |
| 中文关键词: 马铃薯 轮作 连作 土壤有机物 GC-MS分析 |
| 英文关键词:potato rotation continuous cropping soil organic compounds GC-MS analysis |
| 基金项目:国家科技支撑计划课题（2012BAD06B03） |
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| 中文摘要: |
| 对轮作（前3 a均种植玉米）和连作(连续4 a种植马铃薯)条件下马铃薯“大西洋”品种出苗期和落花期根区土壤溶液进行GC-MS测定，研究连作逆境下马铃薯根系分泌物的自毒物质。结果发现，两种根区土壤溶液中匹配度80%以上的有机物均检测出烃、苯、酸、醇、酯、醛、酮，酰胺及其烃衍生物，其中出苗期有13种有机物是连作土壤溶液中独有物质，且含量最高的是丙酸丙酯（22.335%），最低的是2-十一烯醛（0.258%）。落花期连作土壤溶液中新增有机化合物7种，其中十六烷酸丙基酯也称棕榈酸丙基酯是植物自毒物质。比对出苗期和落花期两种土壤溶液有机物，确定12种化合物〔壬烷、丙酸丙酯、辛醛、二丁基羟基甲苯、1,2-苯二甲酸二（2-甲基丙基）酯、2,6-二甲基壬烷、2-辛烯醛、2-十一烯醛、1-十二烯、1-十三烷醇、1-十五碳烯、5-十八烯〕不是马铃薯自毒化合物。落花期连作土壤溶液中独有的N，N-二甲基甲酰胺化合物也是出苗期连作土壤独有物质，其浓度为0.455%（出苗期）和0.699%（落花期），该物质是否为自毒物质尚需进一步研究。 |
| 英文摘要: |
| To identify the potato root secreted auto-toxicity compounds under continues cropping, soil solutions from four years continuous cropping field and three years potato/maize rotation field during seedling and flowering stage were analyzed by the GC-MS. About 80% of organic compounds from both samples contained hydrocarbons, benzenes, acids, alcohols, esters, aldehydes, ketones, amides and the derivatives of hydrocarbons. Among these chemicals 13 kinds were only detected from the soil sampled at the seedling stage of continuous cropping. The highest and lowest abundance of chemicals were detected for n-propyl propionate（22.335%） and 2-undecenal (0.258%), respectively. Additional seven kinds of chemicals were detected from the soil sample at flowering stage, the palmitic acid isopropyl ester was identified as one of the plant auto-toxicities. Compared with flowering stage, seedling stage had twelve chemicals identified as non auto-toxicity of the potato plant, including n-nonane, n-propyl propionate, capryl aldehyde, butylated hydroxytoluene, dimethyl1, 2-benzenedicarboxylate, 2,6-dimethylnonane, 2-octenal,2-undecental, 1-dodecene, 1-tridecanol, 1-pentadecen and 5-octadecylene. Although N, N-dimethyl-formamide was detected during both seedling and flowering stages, with the concentration being 0.445% and 0.669%, respectively, their role still await further investigation. |
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