娄艳,白江平,杨宏羽,高慧娟,张俊莲,王蒂.基于渗透胁迫的马铃薯试管苗抗旱评价体系的构建[J].干旱地区农业研究,2016,34(5):15~26
基于渗透胁迫的马铃薯试管苗抗旱评价体系的构建
Establishment of an evaluation system on drought resistance of potato plantlets based on osmotic stress
  
DOI：10.7606/j.issn.1000-7601.2016.05.03
中文关键词:  马铃薯  水分胁迫  生理生化指标  隶属函数法
英文关键词:potato  water stress  physiological and biochemical indicators  subordinate function method
基金项目:科技部国际科技合作项目(2014DFG31570)；甘肃科技基金(1308RJZA131，1308RJIA005)；兰州科技研究项目(2013-4-156)
作者单位
娄艳 甘肃省作物遗传改良与种质创新重点实验室/甘肃省干旱生境作物学重点实验室， 甘肃 兰州 730070；甘肃农业大学农学院， 甘肃 兰州 730070 
白江平 甘肃省作物遗传改良与种质创新重点实验室/甘肃省干旱生境作物学重点实验室， 甘肃 兰州 730070；甘肃农业大学农学院， 甘肃 兰州 730070 
杨宏羽 甘肃省作物遗传改良与种质创新重点实验室/甘肃省干旱生境作物学重点实验室， 甘肃 兰州 730070；甘肃农业大学园艺学院， 甘肃 兰州 730070 
高慧娟 甘肃省作物遗传改良与种质创新重点实验室/甘肃省干旱生境作物学重点实验室， 甘肃 兰州 730070；甘肃农业大学农学院， 甘肃 兰州 730070 
张俊莲 甘肃省作物遗传改良与种质创新重点实验室/甘肃省干旱生境作物学重点实验室， 甘肃 兰州 730070；甘肃农业大学园艺学院， 甘肃 兰州 730070 
王蒂 甘肃省作物遗传改良与种质创新重点实验室/甘肃省干旱生境作物学重点实验室， 甘肃 兰州 730070；甘肃农业大学农学院， 甘肃 兰州 730070 
摘要点击次数: 1505
全文下载次数: 809
中文摘要:
      为探明抗旱生理生化机制和筛选抗旱品种，建立科学有效的马铃薯实验室抗旱评价体系，本研究分析了不同浓度PEG (polyethylene glycol6000) 对CIP 397098.12、CIP 391180.6、CIP 391724.1、CIP 392745.7、CIP 392759.1、CIP 393613.2、CIP 397035.26、CIP 302476.108、CIP 304345.102、CIP 304405.47、CIP 391930.1、CIP 391931.1、陇薯3号及Atlantic马铃薯品种试管苗生理生化指标的影响，并利用隶属函数分析方法对供试材料的综合抗旱能力进行了评价。结果表明：随着PEG浓度的增加，马铃薯试管苗的地上部分及根系生长受到严重的抑制，对马铃薯试管苗的根系长度的抑制作用更明显。当PEG浓度为9% 时，14个马铃薯品种间生长指标的差异最大，试管苗株高、根系长度等5个生长指标均显著低于对照，说明9%是用于鉴定不同马铃薯品种试管苗对水分胁迫响应的最适浓度。根据综合抗旱评价值对供试马铃薯材料的抗旱性进行了排序，由强到弱依次为：陇薯3号、CIP 391930.1、CIP 397098.12、CIP 391931.1、CIP 304405.47、CIP 392759.1、CIP 397035.26、CIP 393613.2、CIP 302476.108、CIP 304345.102、CIP 391180.6、Atlantic、CIP 391724.1、CIP 392745.7。
英文摘要:
      To explore the physiological and biochemical mechanism of drought resistance and establish an efficient drought-resistant evaluation system on potato plantlets, the effect of the PEG (polyethylene glycol 6000) simulated drought stress on the physiological and biochemical parameters for plantlets of potato varieties including CIP 397098.12, CIP 391180.6, CIP 391724.1, CIP 392745.7, CIP 392759.1, CIP 393613.2, CIP 397035.26, CIP 302476.108, CIP 304345.102, CIP 304405.47, CIP 391930.1, CIP 391931.1, Longshu3 and Atlantic, and the drought-resistant abilities of testing materials were comprehensively evaluated using the method of Subordinate Function Analysis. The results showed that increase of PEG concentration inhibited the growth of potato seedlings, especially the length of root system that was seriously affected. When 9% PEG was applied, five growth indicators of the samples, such as plant height, root length, etc., were significantly lower than those in the control samples. Therefore, 9% PEG6000 was the optimal concentration to simulate water stress for potato seedlings. According to the value of the comprehensive evaluation, drought resistance of these varieties from strong to weak could be ranked as follows: Longshu3>CIP 391930.1>CIP 397098.12>CIP 391931.1>CIP 304405.47>CIP 392759.1>CIP 397035.26>CIP 393613.2>CIP 302476.108>CIP 304345.102>CIP 391180.6>Atlantic>CIP 391724.1>CIP 392745.7.
查看全文  查看/发表评论  下载PDF阅读器