肖红,王芳,段春华,景媛媛,陈陆军,张建文,杨海磊,鱼小军.不同扁蓿豆种质孕蕾期抗旱性综合评价[J].干旱地区农业研究,2016,34(5):62~68
不同扁蓿豆种质孕蕾期抗旱性综合评价
Comprehensive evaluation of drought resistance of different Medicago ruthenica germplasms at the budding stage
  
DOI：10.7606/j.issn.1000-7601.2016.05.09
中文关键词:  扁蓿豆  孕蕾期  抗旱性  隶属函数
英文关键词:Medicago ruthenica  budding stage  drought resistance  subordinate function
基金项目:甘肃省高校基本科研业务费
作者单位
肖红 甘肃农业大学草业学院，草业生态系统教育部重点实验室，中-美草地畜牧业可持续发展研究中心，甘肃省草业工程实验室， 甘肃 兰州 730070 
王芳 甘肃农业大学农学院， 甘肃 兰州 730070 
段春华 四川省宣汉县饲草饲料工作站， 四川 宣汉 636150 
景媛媛 甘肃农业大学草业学院，草业生态系统教育部重点实验室，中-美草地畜牧业可持续发展研究中心，甘肃省草业工程实验室， 甘肃 兰州 730070 
陈陆军 甘肃农业大学草业学院，草业生态系统教育部重点实验室，中-美草地畜牧业可持续发展研究中心，甘肃省草业工程实验室， 甘肃 兰州 730070 
张建文 甘肃农业大学草业学院，草业生态系统教育部重点实验室，中-美草地畜牧业可持续发展研究中心，甘肃省草业工程实验室， 甘肃 兰州 730070 
杨海磊 甘肃农业大学草业学院，草业生态系统教育部重点实验室，中-美草地畜牧业可持续发展研究中心，甘肃省草业工程实验室， 甘肃 兰州 730070 
鱼小军 甘肃农业大学草业学院，草业生态系统教育部重点实验室，中-美草地畜牧业可持续发展研究中心，甘肃省草业工程实验室， 甘肃 兰州 730070 
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中文摘要:
      采用盆栽反复干旱法，在适度、中度和重度水分胁迫(土壤含水量分别为75%～80%、50%～55%和30%～35%)条件下，研究了12份不同生境扁蓿豆（Medicago ruthenica）种质孕蕾期的抗旱性。结果表明:随着干旱胁迫程度的加剧，12份扁蓿豆的绝对株高、茎粗、地上生物量、地下生物量、叶片相对含水量和叶绿素含量均呈下降趋势，而丙二醛（MDA）含量呈上升趋势；与对照相比，中度水分胁迫下，12份扁蓿豆叶片相对含水量的降幅均在11%以下，重度水分胁迫严重抑制了扁蓿豆的生长；中度和重度胁迫下，陇县扁蓿豆绝对株高降幅较小，分别下降了1.8%和22.1%；而渭源扁蓿豆叶片MDA含量在中度和重度水分胁迫下均比其他材料的含量高，分别为26.172 μmol·g-1和31.149 μmol·g-1。以中度和重度水分胁迫处理下绝对株高、茎粗、地上生物量、地下生物量、叶片相对含水量、MDA含量和叶绿素含量的相对值为评价指标，应用隶属函数法对12份扁蓿豆孕蕾期抗旱性进行综合评价，抗旱性强弱依次为：陕西陇县＞榆中＞永昌＞陇西＞天水＞渭源＞土默特＞自选8号＞景泰＞临夏＞镇原＞夏河。
英文摘要:
      With optimal, medium and severe water stresses (soil relative water contents were 75%～80%, 50%～55%, and 30%～35%, respectively), the drought resistances of 12 Medicago ruthenica germplasms from different habitats at the budding stage were studied by a pot culturing method by repeated drought stress. The results showed that plant height, stem diameter, aboveground and underground biomass, leaf relative water content and chlorophyll content were all decreased with the increase in severity of drought stress, while the MDA content was increased. Compared with the control (CK), the decreasing levels of leaf relative water content for 12 M. ruthenica germplasms were all below 11% under medium water stress. The growth of M. ruthenica was seriously inhibited under severe water stress. In addition, under medium and severe water stresses, the plant heights of M. ruthenica (Longxian) were declined by 1.8% and 22.1% respectively, and the leaf MDA contents of M. ruthenica (Weiyuan) were 26.172 μmol·g-1 and 31.149 μmol·g-1, respectively, higher than other materials. Based on the relative values of plant height, stem diameter, aboveground biomass, underground biomass, leaf relative water content, MDA content and chlorophyll content under medium and severe water stresses, the drought resistance of 12 Medicago ruthenica germplasms was comprehensive evaluated by the subordinate function method and followed the order from strong to weak as Longxian (from Shanxi）＞Yuzhong＞Yongchang＞Longxi＞Tianshui＞Weiyuan＞Tumote＞No.8 Zixuan＞Jingtai＞Linxia＞Zhenyuan＞Xiahe.
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