| 偶春,姚侠妹,姚晓洁,纪敬,王魏根,郭健.外源5-氨基乙酰丙酸和PEG处理下栀子幼苗光合及抗氧化特性变化[J].干旱地区农业研究,2016,34(6):235~242 |
| 外源5-氨基乙酰丙酸和PEG处理下栀子幼苗光合及抗氧化特性变化 |
| Changes of photosynthetic and antioxidant properties of Gardenia jasminoides seedlings treated with 5-aminolevulinic acid and PEG |
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| DOI：10.7606/j.issn.1000-7601.2016.06.36 |
| 中文关键词: 栀子 PEG胁迫 5-氨基乙酰丙酸 光合 抗氧化特性 |
| 英文关键词:Gardenia jasminoides PEG stress 5-aminolevulinic acid photosynthesis antioxidant property |
| 基金项目:安徽省高校质量工程项目（2013zy049，2014jyxm231, 2015jyxm729，2015jyxm221）；安徽省高校省级自然科学研究项目（2015KJ004）；安徽省高校省级人文社科研究项目（SK2016A070，SK2015A723）；国家级大学生创新训练计划项目（201610371024）；阜阳师范学院质量工程项目（2014SJJD01）；阜阳师范学院自然科学研究项目（2016FSKJ10，2015FSKJ01ZD） |
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| 中文摘要: |
| 采用溶液培养方式，研究不同浓度（0、25、50、100 mg·L-1和200 mg·L-1)5-氨基乙酰丙酸（ALA）对PEG胁迫条件下栀子幼苗有机渗透调节物质含量、光合生理及抗氧化系统等生理生化指标。结果表明，栀子幼苗PEG胁迫14 d，地上和地下部分干重、苗高、根长、相对含水量、光合色素含量、净光合速率（Pn）、蒸腾速率（Tr）、气孔导度（Gs）和水分利用率（WUE）下降；脯氨酸、丙二醛（MDA）、过氧化氢（H2O2）含量和相对电导率显著上升，而抗氧化酶（SOD、POD、CAT）活性明显下降，一定浓度ALA处理降低了PEG引起栀子幼苗生理上的压力，50 mg·L-1的外源ALA处理可以显著缓解PEG诱导生长抑制，与单独PEG胁迫处理相比，叶绿素a和叶绿素b含量、净光合速率、SOD、POD、CAT活性、相对含水量和脯氨酸的积累分别提高了25.35%、31.85%、228.68%、41.92%、27.26%、41.50%、170.26%、31.01%，MDA含量、H2O2含量和相对电导率分别降低39.00%、31.18%、45.56%。相反，200 mg·L-1 ALA处理则可能加剧干旱胁迫伤害。 |
| 英文摘要: |
| The alleviative effects of exogenous 5-aminolevulinic acid (ALA) on plants against drought stress were assessed in Gardenia jasminoides seedlings treated with ALA at different concentrations. By water culuring, G. jasminoides seedlings were cultured with 15% PEG6000 and ALA with different concentrations (0, 25, 50, 100 mg·L-1 and 200 mg·L-1), and water was used as control. Their physiological and biochemical indexes were studied, including organic osmolytes content, photosynthesis and antioxidant system. The results showed that seedlings exposed to 15% PEG for 14 d exhibited a decrease in aboveground and underground dry mass, seedling height, root length, relative water content, photosynthetic pigment content, net photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs), and water use efficiency. In PEG-stressed plants, the levels of proline, malondialdehyde (MDA), hydrogen peroxide (H2O2), and electrolyte leakage were significantly increased, whereas antioxidative activity, including superoxide, peroxidase, and catalase activities, went declined in leaves. However, the presence of ALA provided an effective method of mitigating PEG-caused physiological stresses on G. jasminoides seedlings, which depended on ALA levels. PEG-treated plants exposed to ALA at 50 mg·L-1 significantly eased PEG-induced growth inhibition. Application of ALA, especially at the concentration of 50 mg·L-1, considerably raised chlorophyll a content, chlorophyll b content, Pn, SOD, POD, CAT activity, relative water content and proline accumulation by 25.35%, 31.85%, 228.68%, 41.92%, 27.26%, 41.50%, 170.26%, and 31.01%, respectively, while decreased MDA content, H2O2 content, and electrolyte leakage by 39.00%, 31.18%, and 45.56%, respectively. By contrast, the positive effects were not evident, or even more severe in the PEG+200 mg·L-1 ALA treatment. |
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