| 李防洲,冶军,侯振安.外源调节剂包衣对低温胁迫下棉花种子萌发及幼苗耐寒性的影响[J].干旱地区农业研究,2017,35(1):192~197 |
| 外源调节剂包衣对低温胁迫下棉花种子萌发及幼苗耐寒性的影响 |
| Effects of seed film coating with exogenous regulating substances on cotton germination rate and cold tolerance of seedlings |
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| DOI：10.7606/j.issn.1000-7601.2017.01.29 |
| 中文关键词: 棉花 种子萌发 外源调节剂包衣 低温胁迫 幼苗耐寒性 |
| 英文关键词:cotton germination rate film coating with exogenous regulating substances low temperature stress cold resistance of seedlings |
| 基金项目:国家科技支撑计划“低温灾害防控关键技术研究与应用”项目(2012BAD20B03) |
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| 中文摘要: |
| 为探讨外源调节剂包衣对低温胁迫棉花幼苗耐寒性的调控效应，通过室内砂培试验，研究了外源调节剂包衣处理对低温下棉花种子萌发的影响，分析了5℃低温胁迫下以及恢复常温后外源调节剂包衣对棉花幼苗耐寒性的生理响应。结果表明：低温胁迫下棉花种子的萌发和幼苗生长受到抑制，水杨酸以及外源调节剂复配包衣处理均能显著提高低温胁迫下棉花种子的发芽势、发芽率、发芽指数和活力指数，其中发芽指数和活力指数达到极显著水平，增幅分别为41.2%～44.4%和51.2%～63.9%；外源调节剂包衣处理叶片的相对电导率(REC)和MDA含量显著降低，可溶性蛋白和可溶性糖等渗透调节物质的含量显著提高，SOD、POD和CAT等抗氧化酶的活性也显著增强；5 mmol·L-1水杨酸+45 mmol·L-1氯化钙+60 mg·L-1亚硒酸钠复配包衣处理的叶片REC、MDA含量显著低于其它包衣处理，可溶性蛋白含量及其增幅显著高于其它包衣处理。说明水杨酸、氯化钙和亚硒酸钠三元复配包衣在缓解棉花幼苗低温逆境中具有协同效应，可以缓解低温胁迫对棉花种子萌发及幼苗生长的抑制作用，提高植物的耐寒性。 |
| 英文摘要: |
| The aim of this experiment was to study the effects of seed film coating with exogenous regulating substances on the cotton seed germination and physiological response of cold tolerance in cotton seedlings under low temperature stress of 5℃ by sand culture method. The results showed that the low temperature stress inhibited the cotton seed germination and growth of seedlings. Seeds coated with salicylic acid and exogenous regulator compound had better germination potential, and higher germination rate, germination index and vigor index. Compared with CK, seeds coated with exogenous regulator compound had germination index increased by 41.2%～44.4%, vigor index 30.9%～63.9%. The coating treatments with exogenous regulating substances decreased leaf REC and MDA content. It also increased sharply the soluble protein and soluble sugar contents. The activity of SOD, POD and CAT were significantly increased. The coating treatment with a compound preparation of 5 mmol·L-1 salicylic acid, 45 mmol·L-1 calcium chloride and 60 mg·L-1 sodium selenite which REC and MDA content were significantly higher than other coating treatment, and soluble protein content were significantly higher than other coating treatment. These results demonstrated that seed film coating with salicylic acid, calcium chloride and sodium selenite has a synergistic effect of improving low temperature resistance of cotton seedling by promoting seed germination and seedling growth. |
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