| 司鹏,于会丽,高登涛,邵微,乔宪生,陈锦永.生草对沙地葡萄园土壤微生物群落碳源利用特征的影响[J].干旱地区农业研究,2017,35(2):247~254 |
| 生草对沙地葡萄园土壤微生物群落碳源利用特征的影响 |
| Effects of intercropping herbages on carbon source metabolism of soil microbial community in sandy vineyard |
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| DOI：10.7606/j.issn.1000-7601.2017.02.40 |
| 中文关键词: 生草 土壤微生物功能多样性 Biolog-Eco技术 |
| 英文关键词:herbage intercropping soil microbial functional diversity Biolog-Eco technology |
| 基金项目:河南省重点科技攻关项目（152102110112）；中央级科研院所基本科研业务费专项(1610192017607)；中国农业科学院科技创新工程专项经费（CAAS-ASTIP-2017-ZFRI |
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| 中文摘要: |
| 为了研究葡萄园行间生草处理对土壤微生物群落功能多样性的影响，以清耕为对照，运用Biolog-Eco板技术，分析生草处理下的土壤微生物群落碳源利用特征。结果表明：生草处理的AWCD值和多样性指数（Shannon-Wiener多样性指数、Simpson优势度指数和丰富度指数）均显著高于清耕对照，且随土层深度的增加均呈下降趋势，以生草0～20 cm土层处理最高；与清耕对照相比，生草处理土壤微生物对6类碳源利用强度均显著增加，平均增幅顺序为：羧酸类化合物>酚酸类化合物>胺类化合物>碳水化合物类>氨基酸类>聚合物。主成份分析表明，清耕对照和生草处理分布在不同区域，表明两处理土壤微生物存在一定的代谢差异性，生草显著改变了土壤微生物群落功能，并对土壤微生物代谢羧酸类化合物、碳水化合物和酚酸类化合物3类碳源的影响最为明显。结果提示，生草有利于葡萄园土壤微生物群落结构的改善。 |
| 英文摘要: |
| In order to study the functional diversity of soil microbial community in vineyard intercropping with herbage, the Biolog-Eco technology was used to analyze the utilization features of carbon sources by microbial community, with clean cultivation as the control. The results revealed that the average well color development (AWCD) and diversity indices (Shannon-Wiener index, Simpson index and richness index) under herbage intercropping were significantly increased compared with the control, and they decreased as soil depth increasing, with the highest values in 0～20 cm soil layer under herbage intercropping. Additionally, the utilization intensity of 6 carbon substrates was enhanced by herbage intercropping, and the increase rate was ranked as: carboxylic acids＞phenolic acid＞amines＞carbohydrates＞amino acids＞polymer. The principal component analysis showed that samples of control and herbage intercropping were distributed in different zones, which meant that the function of soil microbial community was obviously altered by herbage intercropping. Carboxylic acids, carboh
ydrates and phenolic acids were the most sensitive carbon sources in the aspect of soil microbial metabolism induced by herbage intercropping. Overall, herbage intercropping could not only enrich the diversity of microbial community, but also improve its structure in sandy vineyard. |
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