| 王萍,张希吏,石磊.干旱胁迫下沙芥幼苗叶片光合特性和叶绿素荧光参数的变化[J].干旱地区农业研究,2017,35(3):159~163 |
| 干旱胁迫下沙芥幼苗叶片光合特性和叶绿素荧光参数的变化 |
| Changes of photosynthetic pharacteristics and chlorophyll fluorescence parameters of Pugionium cornutum(L.) Gaertn seedlings leaf under drought stress |
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| DOI：10.7606/j.issn.1000-7601.2017.03.25 |
| 中文关键词: 沙芥幼苗 干旱胁迫 光合参数 叶绿素荧光参数 |
| 英文关键词:Pugionium cornutu(L.) Gaertn seedling drought stress photosynthetic parameters chlorophyll fluorescence parameters |
| 基金项目:国家自然科学基金项目(31260475，30960236，30560088)；内蒙古农业大学创新(培育)团队(NDPYTD2013-3) |
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| 中文摘要: |
| 以一年生沙芥幼苗的叶片为实验材料，采用盆栽实验的方法，将土壤含水量设置为正常浇水为对照（70%～75%WHC，CK），轻度干旱胁迫（55%～60%WHC，LD），中度（40%～45%WHC，MD），重度（25%～30%WHC，VD）四个处理，用Li-6400 光合仪和叶绿素荧光仪FMS2(Hansatech, UK)，研究干旱胁迫下沙芥光合参数、荧光参数的变化规律，探讨干旱胁迫对沙芥叶片光合生理指标的影响，旨在揭示沙芥对干旱胁迫的响应机制，为阐明沙芥的耐旱性提供理论依据。结果表明：(1) 随着干旱胁迫程度的加剧，沙芥叶片叶绿素a,b含量在各干旱胁迫处理下，比对照下降了1.30%～7.04%和19.47%～42.37%，类胡萝卜素含量下降了2.06%～9.88%。叶绿素a/b上升了33.49%～74.27%，Car/Chl上升了1.33%～15.73%。(2) 随着干旱胁迫的加剧，沙芥叶片的净光合速率、蒸腾速率、水分利用率呈现下降的趋势，胞间CO2浓度先下降后上升，气孔限制值不断下降。这表明轻度干旱胁迫下气孔限制是影响沙芥幼苗叶片Pn下降的主要因素;中度和重度干旱胁迫下，非气孔限制是沙芥幼苗叶片Pn下降的主要因素。(3) 干旱胁迫下，沙芥叶绿素荧光参数Fv/Fm、Fv/Fo、ΦPSⅡ，qP和ETR均呈下降趋势，qN在干旱胁迫下不断上升。 |
| 英文摘要: |
| With the help of the method of a pot-cultivation experiment and the reference of different soil content with 70%～75%WHC(CK), 55%～60%WHC(LD), 40%～45%WHC(MD), 25%～30%WHC(VD) and with FMS-2 and Li-6400 photosynthetic apparatus, choosing seedling leaves of Pugionium cornutum(L.) Gaertn as the experimental materials, this paper studies the change laws of hlorophyll fluorescence parameters of Pugionium cornutum(L.) Gaertn under the drought stress, and the effects of drought stress on its photosynthetic index. The purpose of this paper is to reveal the mechanism of Pugionium cornutum(L.) Gaertn dealing with the drought stress, and to illustrate its drought resistance. The results showed that: (1) With intensifying drought stress, by contrast, the content of Pugionium cornutum leaf chlorophyll a, chlorophyll b in P. cornutum(L.) Gaertn decreased by 1.30%～7.04% and 19.47%～42.37% respectively, carotene content by 2.06%～9.88%; the value of chlorophyll a/b and Car/Chl increased by 33.49%～74.27% and 1.33%～15.73% respectively. (2) With intensifying the drought stress, there were distinct decrease trends of the net photosynthetic rate(Pn), transpiration rate(Tr), stomatal conductance(Gs) and water use efficiency(WUE) of P. cornutum(L.) Gaertn leaves, and the carbon dioxide concentration decrease at first and then increase and the stomatal limitation(Ls) decrease, which showed that Pn decrease of P. cornutum(L.) Gaertn leaves under light stress was mainly induced by stomatal factors, and Pn decrease under moderate and severe stress was mainly induced by non-stomatal factors. (3) Under drought stress, there were decrease trends of Fv/Fm, Fv/Fo,ΦPSⅡ, qP, ETR of Pugionium cornutum(L.) Gaertn leaf, and increase trends of qN. |
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