| 王鹤,彭楚,来航线,韦小敏,姜影影.太白山土壤放线菌分离方法及生态分布规律[J].干旱地区农业研究,2017,35(5):107~114 |
| 太白山土壤放线菌分离方法及生态分布规律 |
| Study on method of actinomycetes isolation from soils of Taibai mountain and its ecological distribution |
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| DOI：10.7606/j.issn.1000-7601.2017.05.16 |
| 中文关键词: 土壤养分 放线菌 分离方法 生态分布 太白山 |
| 英文关键词:soil nutrient actinomycetes isolation method ecological distribution Taibai mountain |
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| 中文摘要: |
| 研究了改良高氏培养基、甘油-天门冬酰胺培养基、R2A培养基、改良高氏二号培养基、海藻糖-脯氨酸培养基对太白山不同植被土壤放线菌的分离效果，并分析放线菌的生态分布规律。结果表明：太白山南北坡土壤养分大体呈现为中海拔林地含量相对较高，有机质和全氮含量最高分别为77.81 g·kg-1和5.35 g·kg-1，低海拔林地与高海拔林地含量相对较低，有机质和全氮含量最低分别为11.77 g·kg-1和0.85 g·kg-1，即自低海拔至高海拔不同林地类型土壤养分整体呈现先升高后降低的趋势；甘油-天门冬酰胺培养基分离的放线菌数量与种类均高于其它培养基，其数量最高可达258×104 cfu·g-1土，种类最多为35种；改良高氏培养基分离南坡的效果优于北坡；R2A培养基分离中高海拔林地土壤优势更明显。中海拔林地类型，如北坡的红皮桦、牛皮桦，南坡的牛皮桦，分离的放线菌数量均显著高于其它林地类型；土壤速效磷和全氮含量对放线菌的分离效果影响较大。 |
| 英文摘要: |
| The study explored the method of actinomycetes isolation from soils of Taibai mountain through five medias, including modification No.1 media、glycerin-asparagine media、R2A media、modification No.2 mediaand trehalose-proline media,and analyse its ecological distribution. The result showed that, soil nutrient of Taibai mountain on both north and south slope presented a relatively high concentration in mid-elevation forest type, the maximum concentration of organic matter and total nitrogen was 77.81 g·kg-1 and 5.35 g·kg-1, respectively, a relatively low concentration in the both sites of altitude, organic matter and total nitrogen concentration were lowest at 11.77 g·kg-1 and 0.85 g·kg-1, respectively, which means from low to high elevation in different forest types soil nutrient presented a trend of reducing then rising as a whole; Analysis of actinomycetes number and variety on different soils of plant types showed that modification glycerin-asparagine media was apparently higher than that from others, with the quantity of 258×104 cfu·g-1 soil, 35 species kinds, Modification No.1 media showed a better separation effect on the south slope, which mean more suitable for the separation of the south slope of different forest soil actinomyces. There are obvious advantages of R2A media while isolating mid-elevation forest type. Mid-elevation forest type, such as red birch、betula utilise in north slope, betula utilise in south slope, represented a significant difference than other types on the number of isolated actinomycetes. Soil available phosphorus and total nitrogen concentration had a greater influence on the number and variety isolated from soils, slope affected the correlation between isolation result and soil nutrients. |
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