| 黄倩,赵永国,黄祥伟,朱宗河,刘云清,马海清,程勇,邹锡玲,徐劲松,张学昆,陆光远.甘蓝型油菜蕾薹期抗旱相关性状的QTL分析[J].干旱地区农业研究,2017,35(6):88~94 |
| 甘蓝型油菜蕾薹期抗旱相关性状的QTL分析 |
| QTL mapping of traits associated with drought resistance at bolting stage in Brassica napus |
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| DOI：10.7606/j.issn.1000-7601.2017.06.14 |
| 中文关键词: 甘蓝型油菜 蕾薹期 遗传连锁图 抗旱性 QTL分析 |
| 英文关键词:Brassica napus bolting stage genetic linkage map drought resistance QTL analysis |
| 基金项目:国家自然科学基金项目(31371663)；湖北省农业科技创新中心项目，中国农业科学院科技创新工程 |
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| 中文摘要: |
| 蕾薹期是甘蓝型油菜对干旱最为敏感的时期之一。以抗旱品系QY8-1和干旱敏感品种沪油16构建的含183个家系的F2∶3群体为研究材料，在现蕾至抽薹期进行干旱胁迫处理，正常灌溉为对照。结果表明，与对照相比，干旱胁迫后群体的抽薹高度、单株鲜重和叶片萎蔫指数平均下降了52.6%～55.3%，而且三个指标高度正相关。利用335个SRAP标记构建了一个长度为1661.9 cM的遗传图谱，通过复合区间作图法共检测到28个QTL，分布在14个连锁群上，可解释1.1%～36.6%的表型变异。其中，利用抽薹高度共检测到10个QTL，位于第15连锁群的qSH-D-5可解释36.6%的表型变异，为主效QTL。该区间还检测到其它3个QTL（qSH-DRI-1、qSH-D-5和qLWI-D-1），在抗旱分子标记辅助选择中应予以重点关注。同样，在第16连锁群（标记区间Em01/Me12-1—Em01Me09-1）也检测到4个重合的QTL，贡献率为1.1%～8.1%，为微效QTL。 |
| 英文摘要: |
| Bolting is one of the most sensitive stages to drought stress in Brassica napus. To identify quantitative trait loci(QTL) associated with drought resistance at bolting stage, we generated a F2∶3 populations with 183 lines by crossing drought-resistant line QY8-1 with the drought-sensitive one Huyou16. This population was subjected to two treatments, namely drought stress from bud emerging to bolting stages and well-water at the same stages. The results showed that shoot height, single plant fresh weight and leaf wilting index were all decreased by 52.6%～55.3% in average under drought stress compared to the control. Moreover, the three traits were highly positively correlated, indicating that they are suitable drought-resistant indicators. A linkage map was constructed with 335 SRAP markers, covering a total length of 1661.9 cM. By using composite interval mapping method, a total of 28 QTLs were detected across 14 linkage groups (LG), accounting for 1.1% to 36.6% of trait variation. Among these, 10 QTLs were identified for shoot height, and the one in LG15 (qSH-D-5) was a major QTL accounting for 36.6% of variation. There were three other QTLs（qSH-DRI-1、qSH-D-5 and qLWI-D-1） overlapping this interval, thus being an important genetic region for breeding selection via molecular markers. Meanwhile, there were also 4 minor QTLs overlapping at marker interval Em01/Me12-1—Em01Me09-1 in LG16, only accounting for 1.1%～8.1% of trait variation. |
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