| 邢潇晨,索琳格,肖春燕,刘苗苗,刘慧英,崔金霞.5-氮杂胞苷对低温胁迫下黄瓜幼苗光合作用的影响[J].干旱地区农业研究,2017,35(6):116~123 |
| 5-氮杂胞苷对低温胁迫下黄瓜幼苗光合作用的影响 |
| Effect of 5-azacytidine on photosynthesis of cucumber seedlings under low temperature and light intensity |
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| DOI：10.7606/j.issn.1000-7601.2017.06.18 |
| 中文关键词: 黄瓜幼苗 5-氮杂胞苷 低温 光合作用 耐冷性 |
| 英文关键词:cucumber seedling 5-azaC low temperature photosynthesis cold tolerance |
| 基金项目:国家自然科学基金项目(31160404)；石河子大学高层次人才科研启动资金专项(RCZX201213) |
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| 中文摘要: |
| 为探究降低基因组DNA甲基化水平在低温条件下对黄瓜幼苗光合作用的影响，以‘津研四号’黄瓜幼苗为试材，研究了不同浓度DNA甲基化抑制剂5-azaC处理对低温（10℃/6℃，300μmol·m-2·s-1）胁迫下黄瓜幼苗叶片气体交换参数、叶绿素荧光猝灭、细胞膜透性的影响。结果表明：低温条件下，500～800 μmol·L-1的5-azaC处理能够显著降低黄瓜幼苗叶片细胞膜透性（P<0.05）；提高叶片净光合速率（Pn）、气孔导度（Gs）、蒸腾速率（Tr），降低胞间CO2浓度（Ci）；同时也促进PSⅡ的最大光化学效率（Fv/Fm）、实际光化学效率（ΦPSⅡ）、光合电子传递效率（ETR）和光化学猝灭系数（qP）的升高，非光化学猝灭系数（NPQ）和叶片光化学猝灭参数［Y(NO)］的降低，从而提高黄瓜幼苗的耐冷性。 |
| 英文摘要: |
| The objective of this study was to explore the effect of reduction in methylation level of genomic DNA on photosynthesis of cucumber seedlings under low temperature. ‘Jinyan No.4’ cucumber seedlings was used as the experimental materials, and the effects of different concentrations of 5-azaC on the leaf gas exchange parameters, chlorophyll fluorescence quenching and cell membrane permeability of cucumber seedlings under low temperature (10℃/6℃, 300 μmol·m-2·s-1) were studied. The results showed that the net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), the maximum photochemical efficiency of PSⅡ(Fv/Fm), the actual photochemical efficiency (ΦPSⅡ), the photosynthetic electron transport efficiency (ETR) and the photochemical quenching coefficient (qP) of the cucumber seedlings leaf were increased, while leaf cell membrane permeability, intercellular CO2 concentration (Ci), the non-photochemical quenching coefficient (NPQ) and the leaf photochemical quenching parameters ［Y(NO)］ were reduced by 500 to 800 mol·L-1 of 5-azaC treatment under low temperature, thus improving the cold tolerance of cucumber seedlings. |
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