盐胁迫下宁夏枸杞根系Na<sup>+</sup>、K<sup>+</sup>平衡及抑制剂对其影响的研究

朱志明; 毛桂莲; 许兴; 王盛; 郑蕊; 杨淑娟

朱志明,毛桂莲,许兴,王盛,郑蕊,杨淑娟.盐胁迫下宁夏枸杞根系Na⁺、K⁺平衡及抑制剂对其影响的研究[J].干旱地区农业研究,2017,35(6):140~145

盐胁迫下宁夏枸杞根系Na⁺、K⁺平衡及抑制剂对其影响的研究

Effect of salt stress and inhibitor on uptake and transportation of Na⁺ and K⁺ in the root of Ningxia Lycium barbarum L.

DOI：10.7606/j.issn.1000-7601.2017.06.21

中文关键词: NaCl胁迫枸杞非损伤微测技术 Na⁺ K⁺ 离子平衡抑制剂

英文关键词:NaCl stress Lycium barbarum L. non-invasive micro-test technology Na⁺ K⁺ ion homeostasis inhibitors

基金项目:国家自然科学基金项目资助(31360168,31360361)

作者	单位
朱志明	宁夏大学农学院，宁夏银川 750021
毛桂莲	宁夏大学生命科学学院，宁夏银川 750021
许兴	宁夏大学西北退化生态系统恢复与重建教育部重点实验室，宁夏银川 750021
王盛	宁夏大学生命科学学院，宁夏银川 750021
郑蕊	宁夏大学生命科学学院，宁夏银川 750021
杨淑娟	宁夏大学生命科学学院，宁夏银川 750021

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中文摘要:

以宁夏枸杞为试验材料，利用非损伤微测技术及原子吸收分光光度法，研究NaCl胁迫下枸杞根系中Na⁺、K⁺吸收转运及质膜Na⁺/H⁺逆向转运蛋白抑制剂阿米洛利和质膜H⁺-ATPase抑制剂矾酸钠对宁夏枸杞根系离子平衡的影响。结果表明：随着NaCl胁迫浓度的增加，枸杞根系中Na⁺含量总体升高，与对照差异不显著；K⁺含量呈先升后降趋势；Na⁺/K⁺呈先降后升趋势，且低于对照；Na⁺外排先增加后减少，K⁺外排降低，且显著高于对照。随着胁迫时间的延长，枸杞根系中Na⁺、K⁺含量增加，Na⁺外排逐渐减慢，K⁺外排增加；阿米洛利与矾酸钠均显著降低了盐诱导下的Na⁺和H⁺内流。NaCl胁迫下宁夏枸杞根系借助于其质膜上的Na⁺/H⁺逆向运输系统，将胞内过量的Na⁺排出胞外，同时抑制K⁺外排，保持细胞内较低的Na⁺/K⁺，维持胞内Na⁺、K⁺平衡，从而表现出较强的耐盐性。

英文摘要:

We detected absorption and transportation of Na⁺, K⁺ in the root of Ningxia Lycium barbarum L. under NaCl stress by using non-invasive micro-test technology and atomic absorption spectrophotometry. The results showed that the root exhibited a higher capacity to induce the Na⁺ efflux compared with that of control. The higher the concentration of NaCl, the more Na⁺ accumulated in the root, but have no significant difference with CK. The K⁺ content increased first and then declined, and the Na⁺/K⁺ ratio showed the opposite trend. The longer duration of NaCl treatment, the more Na⁺ and K⁺ accumulated. At the mean time, the lower Na⁺ efflux and higher K⁺ efflux were also observed in the root compared with that of control. These results suggested that initial salt stress enhanced the salt-elicited Na⁺ efflux but inhibited K⁺ efflux to maintain the lower Na⁺/K⁺ ratio and cellular Na⁺/K⁺ homeostasis. The NaCl-induced Na⁺ efflux and H⁺ influx were inhibited by amiloride (a Na⁺/H⁺ antiporter inhibitor) or sodium orthovanadate (a plasma membrane H⁺-ATPase inhibitor), indicating that Na⁺ extrusion in the root of Ningxia Lycium barbarum L. arises from the active Na⁺/H⁺ antiporter across the plasma membrane.

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