| 白生文,许耀照,张文斌,陈修斌,祖廷勋,李翊华,张芬琴.光质对甜椒幼苗生长生理及抗氧化性的影响[J].干旱地区农业研究,2017,35(6):146~153 |
| 光质对甜椒幼苗生长生理及抗氧化性的影响 |
| Effects of blue and purple light on the growth, photosynthetic characteristics and antioxidative activities in yellow sweet pepper seedling |
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| DOI：10.7606/j.issn.1000-7601.2017.06.22 |
| 中文关键词: 光质 黄色甜椒 幼苗生长 生理生化特性 光合特性 叶绿素荧光参数 |
| 英文关键词:light quality yellow sweet pepper seedling growt photosynthetic characteristics chlorophyll fluorescence parameters |
| 基金项目:国家自然科学基金项目(31560072)；甘肃省高校协同创新科技团队支持计划资助(2017C-18)；甘肃省科技支撑计划项目—农业类(1304NKCG137)；河西学院校长基金项目(XZ2015-13) |
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| 中文摘要: |
| 采用基质培养法，研究了LED蓝光和紫光对黄色甜椒幼苗生长及其光合特性、叶绿素荧光参数和抗氧化能力等生理生化特征的影响。结果表明：(1) 与白光处理相比，蓝光处理使黄色甜椒的幼苗株高、茎粗、鲜重、干重、叶片气孔开度、蒸腾速率、气孔导度、光合速率、胞间CO2浓度、瞬时水分利用效率、瞬时羧化效率、瞬时光能利用率、PSⅡ光合性能指数（PI）、抗坏血酸（ASA）和还原型谷胱甘肽（GSH）含量明显增加，其中增加幅度较大的是植株干重、叶片蒸腾速率、光合速率、瞬时光能利用率和抗坏血酸（ASA）含量，其值均在白光处理的2倍以上。与此同时，叶片中超氧阴离子（O2-·）产生速率、过氧化氢（H2O2）和膜质过氧化产物（TBARS）积累分别降低到白光处理的50.26%、44.60%和55.88%； (2) 紫光处理使黄色甜椒幼苗叶片中的类胡萝卜素含量增至白光处理的近2倍，增幅显著，但使其株高、根长、茎粗、鲜重、干重、叶片数、叶面积、叶绿素a含量、叶绿素b含量、叶绿素a+b含量、蒸腾速率、气孔导度、光合速率、胞间CO2浓度、瞬时羧化效率、瞬时光能利用率和最小荧光（Fo）值明显降低，其中干重、蒸腾速率、气孔导度和SOD活性降幅较大，分别为白光处理的11.36%、47.44%、35.86%和49.04%； (3) 蓝光和紫光都没有影响叶片的叶绿素荧光参数PSⅡ最大量子产额（Fv/Fm）和PSⅡ潜在活性（Fv/Fo）。综上，在3种LED光中，蓝光更有利于黄色甜椒幼苗的生长，紫光具有明显的抑制作用。 |
| 英文摘要: |
| Effects of white, blue and purple light from LED on plant growth and development, chlorophyll fluorescence parameters, and other physiological characteristics were examined in the seedlings of yellow sweet pepper using substrate culturing method.It showed that,on the one hand, compared to the white light control, the treatment with blue light could improve most of the physiological and biochemical characteristics tested, including RuBP carboxylase activity, chlorophyll fluorescence parameters, lipid peroxidation, reactive oxygen species (ROS) accumulation, the activities of antioxidant enzymes, as well as the contents of antioxidant substances, such as ascorbic acid (ASA) and reduced glutathione (GSH), in the leaves. Blue light treatment also increased stem height and diameter, plant dry and fresh weights, root numbers, stomata opening, transpiration rate (Tr), stomatal conductance (Gs), net photosynthetic rate (Pn), intercellular CO2 concentration (Ci), instantaneous water utilization efficiency (IWUE), carboxylation efficiency (CE) and light utilization efficiency (LUE), PS Ⅱ photosynthetic performance index (PI), the contents of ASA and GSH, the activities of RuBP carboxylase and antioxidant enzymes of leaves. Moreover, blue light treatment promoted bud formation and flowering, whereas it suppressed leaf stomatal limiting value (Ls), superoxide anion (O2-·) production rate, the accumulation of H2O2 and lipid peroxidation products (TBARS). However, the root long, branch number, leaf area and photosynthetic pigment content were not affected by blue light. On the other hand, purple light treatment showed the almost opposite effects compared to blue light, for example, it reduced the stem height and diameter, plant dry and fresh weights, the numbers of root and leaf, root length and leaf area. Purple light also suppressed the contents of chlorophyll a,chlorophyll b and chlorophyll a+b, stomata opening, Tr, Gs, Pn, Ci, IWUE, CE and LUE, Fo, the activities of RuBP carboxylase and antioxidant enzymes in the leaves. In addition, purple light treatment increased the carotenoid content, PI, and the accumulation of O2-·, H2O2 and TBARS. However, both treatment of blue and purple light did not influence the maximum number of PSⅡ quantum yield (Fv/Fm) and potential activity of PSⅡ (Fv/Fo).The results overall suggest that blue light promoted the growth and development but purple light displayed inhibitory effects in yellow sweet pepper. |
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