王超,李刚,谢越盛,高彦林,郭坚华,许泉.植物根围促生细菌菌剂增强玉米干旱耐受性研究[J].干旱地区农业研究,2018,36(1):126~132
植物根围促生细菌菌剂增强玉米干旱耐受性研究
Enhanced drought tolerance in maize conferred by a consortium of plant growth-promoting rhizobacterium strains
  
DOI：10.7606/j.issn.1000-7601.2018.01.20
中文关键词:  玉米  干旱耐受性  植物根围促生细菌  脱落酸
英文关键词:maize  drought tolerance  plant growth-promoting rhizobacterium (PGPR)  abscisic acid (ABA)
基金项目:江苏省科技厅产学研合作前瞻性项目“防治蔬菜花叶病毒的广谱、高效生物农药创制及关键技术研究”（BY2014128-05）；国家重大科技专项项目“东江上游典型集水区水环境风险控制技术集成与综合示范”(2014ZX07206001)
作者单位
王超 南京农业大学， 江苏 南京 210095； 环境保护部南京环境科学研究所， 江苏 南京 210042 
李刚 环境保护部南京环境科学研究所， 江苏 南京 210042 
谢越盛 南京农业大学， 江苏 南京 210095 
高彦林 南京农业大学， 江苏 南京 210095 
郭坚华 南京农业大学， 江苏 南京 210095 
许泉 南京农业大学， 江苏 南京 210095 
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中文摘要:
      选用植物根围促生细菌（PGPR）菌剂BBS处理玉米幼苗，研究干旱胁迫下玉米幼苗叶片相关生理特性及基因表达差异。结果表明，干旱胁迫15 d后，BBS处理组玉米幼苗的萎蔫程度显著低于对照组；处理组植株叶片MDA含量仅为对照组的74.35%，叶片脯氨酸和可溶性糖含量分别是对照组的3.39倍和1.07倍；处理组植株叶片ZmP5CS1的表达水平及SOD活性均显著高于对照组； BBS处理组植株叶片在干旱胁迫过程中一直保持较低的H2O2含量。研究得出，BBS处理能够有效减缓干旱胁迫对玉米幼苗造成的伤害，进而加速正常浇水后的恢复。处理组植株叶片NCED1和ZmDREB2.7的高水平表达也表明干旱胁迫下玉米幼苗与BBS的互作过程可能有依赖ABA信号途径及不依赖ABA信号途径的共同参与。
英文摘要:
      A consortium of plant growth-promoting rhizobacterium (PGPR) strains BBS was demonstrated to enhance tolerance to drought stress in plants. Here, under drought stress, physiological characteristics and transcription of stress-related genes in leaves of maize plants treated with BBS were investigated. After withholding watering for 15 days, BBS-treated maize seedlings had substantially lighter wilt symptoms than control plants. In relation to the control, BBS treatment decreased the leaf monodehydroascorbate (MDA) content by 25.65%, and increased the leaf proline and soluble sugars content by 3.39-fold and 7.28%, respectively. It activated significantly higher transcription of ZmP5CS1 gene, and significantly enhanced the activity of superoxide dismutase (SOD), a major antioxidant enzyme. Additionally, BBS inoculated plants had significantly lower leaf H2O2 content during drought stress than the control plants. Consequently, BBS efficiently extenuated the drought-induced injury in maize seedlings and facilitated post-drought recovery. Moreover, the stimulated expression of NCED1 and ZmDREB2.7 indicated the involvement of ABA-dependent and ABA-independent signaling pathways in BBS-maize interaction under water deficit.
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