| 赵振宁,赵宝勰.不同大豆品种在萌发期对干旱胁迫的生理响应及抗旱性评价[J].干旱地区农业研究,2018,36(2):131~136 |
| 不同大豆品种在萌发期对干旱胁迫的生理响应及抗旱性评价 |
| Physiological response and drought resistance evaluation of different soybean varieties to drought stress at germination stage |
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| DOI：10.7606/j.issn.1000-7601.2018.02.20 |
| 中文关键词: 大豆 萌发期 生理指标 隶属函数法 |
| 英文关键词:soybean germination stage physiological characteristics membership function method |
| 基金项目:优质大豆新品种选育项目(HK20121-2-007A) |
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| 摘要点击次数: 1395 |
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| 中文摘要: |
| 对3个大豆品种（黄豆、晋豆19及郑9007）采用不同浓度PEG-6000(0、12%、14%、16%、18%和20%)模拟干旱胁迫处理，测定种子萌发及抗氧化酶活性等指标。结果表明，不同浓度PEG处理均降低了3个大豆品种的相对发芽势、相对发芽率及相对胚根长，18% PEG处理为合适的干旱胁迫条件。在18% PEG处理下，3个大豆品种胚根的超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、可溶性糖、可溶性蛋白、脯氨酸及丙二醛(MDA)含量均发生了显著变化。其中，黄豆胚根SOD活性、POD活性、CAT活性、可溶性糖、可溶性蛋白、脯氨酸含量分别为对照的5.41、2.13、2.64、2.84、2.13倍和10.98倍，均高于晋豆19和郑9007，而MDA含量上升为对照的1.59倍，低于晋豆19和郑9007。通过隶属函数法综合评价发现，3个大豆品种的抗旱性强弱依次为黄豆>郑9007>晋豆19，说明黄豆的抗旱能力高于晋豆19和郑9007。 |
| 英文摘要: |
| In our present study, the drought resistance of three soybean varieties which were Huangdou, Jindou 19 and Zheng 9007 were studied in different concentrations (0, 12%, 14%, 16%, 18% and 20%) of PEG-6000. The germination indices, changes of physiological and biochemical indices were studied under both normal water supply and simulated drought conditions. The results showed that PEG-6000 treatment reduced the relative germination potential, relative germination rate and relative radicle length of three soybean varieties, and 18% PEG-6000 was the suitable drought stress condition for drought resistance evaluation. The activity of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), the content of soluble sugar content, soluble protein content, proline content and malondialdehyde (MDA) in three cultivars were significantly changed under 18% PEG-6000 treatment. The SOD activity, POD activity, CAT activity, soluble sugar content, soluble protein content and proline content in radicle of Huangdou were 5.41, 2.13, 2.64, 2.84, 2.13 and 10.98 times higher than that of the control, respectively, which were higher than that of Jinzou 19 and Zheng 9007, however, the MDA content in radicle of Huangdou were 1.59 times higher than that of control, which was lower than Jindou 19 and Zheng 9007. The results of membership function analysis showed that the rank of drought resistance of three soybean varieties was Soybean＞Zheng 9007＞Jindou 19, which indicated that Huangdou has higher drought resistance ability than that of Jindou 19 and Zheng 9007. |
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