| 章英才,柴雅红,曹金霞.灵武长枣果实多糖中单糖组成分析[J].干旱地区农业研究,2018,36(2):144~152 |
| 灵武长枣果实多糖中单糖组成分析 |
| Monosaccharide composition of polysaccharides in Ziziphus jujuba Mill cv. lingwuchangzao fruit |
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| DOI：10.7606/j.issn.1000-7601.2018.02.22 |
| 中文关键词: 灵武长枣 果实 多糖 单糖组成 发育时期 |
| 英文关键词:Ziziphus jujuba Mill cv.lingwuchangzao fruit polysaccharides monosaccharide composition |
| 基金项目:国家自然科学基金项目(31160057) |
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| 中文摘要: |
| 以灵武长枣果实为试验材料，采用热水浸提法、分光光度法及气相色谱-质谱联用法等，分别对不同发育时期的灵武长枣果实进行多糖提取及单糖成分分析，研究不同发育时期灵武长枣果实多糖含量变化规律及其单糖组成成分。结果表明：采用热水浸提法提取不同发育时期灵武长枣果实粗多糖，其得率分别为：膨大前期0.778%，快速膨大期0.865%，着色期0.983%，完熟期1.795%；不同发育时期的灵武长枣果实粗多糖经DEAE-52纤维素离子交换柱层析，均得到Ju-0、Ju-1、Ju-2、Ju-3四个多糖级分，各多糖级分分别过HW-55分子排阻柱后得各精制多糖。采用气相色谱-质谱联用法（GC-MS）测得不同发育时期灵武长枣果实精制多糖中单糖组成及含量均为阿拉伯糖、半乳糖、鼠李糖含量较高，葡萄糖含量次之，甘露糖、木糖含量较低，但其含量在各组分中有差异；此外，根据NIST谱库对照，可知不同发育时期灵武长枣果实精制多糖中还可能含有核糖、岩藻糖、葡萄糖醛酸、半乳糖醛酸，均不含有果糖。因此，粗多糖得率和含量随着果实的发育进程呈现上升趋势，在完熟期达到最高值1.795%；不同发育时期灵武长枣果实精制多糖中多糖含量因级分含量不同而差异较大，其单糖组成及含量也各不相同。 |
| 英文摘要: |
| The fruit of Ziziphus jujuba Mill cv. lingwuchangzao in different developmental periods was used as material to study the change of polysaccharides content and monosaccharide composition, using methods of hot water extraction, spectrophotometry and gas chromatography-mass spectrometry. The main research results are as follows: the crude polysaccharides in Ziziphus jujuba Mill cv. lingwu-changzao fruits of different developmental periods were extracted by hot water extraction, the crude polysaccharides obtaining ratio is different, the crude polysaccharides obtaining ratio in the early bulking period is 0.778%, the rapid enlargement period 0.865%, the coloring period 0.983%, and the fruit maturation period 1.795%. The crude polysaccharide at different developmental periods was fractionated to four fractions (Ju-0、Ju-1、Ju-2、Ju-3) by DEAE-52 cellulose ion-exchange columns, and was purified to be refined polysaccharides by HW-55S molecular size exclusion chromatography. Finally, the refined polysaccharides in the different developmental periods of Ziziphus jujuba Mill cv. lingwuchangzao were analysed by the GC-MS, the monosaccharide composition and content show that the contents of arabinose, galactose and rhamnose are higher, followed by glucose, mannose and xylose, but the monosaccharide composition and contents varied with polysaccharides component. In addition, the refined polysaccharide in the different developmental periods of Ziziphus jujuba Mill cv. lingwuchangzao also contained ribose, fuctose, glucuronic acid and galacturonic acid. In summary, the crude polysaccharide obtaining ratio and contents present ascendant trend with the development of the fruits, and reach the peak 1.795% at the fruit maturation period. The refined polysaccharide contents of Ziziphus jujuba Mill cv. lingwuchangzao fruits at different developmental periods varied according to component. |
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