| 黄海娜,杨金宇,李红旭,刘小勇,叶振风,朱立武,衡伟.灌水处理对黄冠梨叶片结构和激素含量及PbDREB基因表达的影响[J].干旱地区农业研究,2018,36(3):17~22 |
| 灌水处理对黄冠梨叶片结构和激素含量及PbDREB基因表达的影响 |
| Effects of different irrigation mode on leaf structure, hormone content, and the gene PbDREB expression of Huangguan pear |
| |
| DOI：10.7606/j.issn.1000-7601.2018.03.03 |
| 中文关键词: 黄冠梨 节水灌溉 叶片组织结构 激素 DREB |
| 英文关键词:Huangguan pear water\|saving irrigation leaf tissue hormone DREB |
| 基金项目:国家现代农业产业技术体系建设专项（CARS[CD*2]29[CD*2]14）；国家自然科学基金（31101519） |
|
| 摘要点击次数: 1087 |
| 全文下载次数: 575 |
| 中文摘要: |
| 试验以9年生‘黄冠’梨为试验材料，对6种不同灌溉处理的叶片组织结构、激素含量及抗旱基因PbDREB表达量进行测定分析。结果表明，灌水量相同，叶片厚度、角质层厚度、栅栏组织厚度、叶片结构紧密度与灌水次数或灌水深度呈正相关。灌水量与抗旱基因PbDREB表达量呈显著正相关。覆膜滴灌[CD*2]灌水9次[CD*2]下渗60 cm、年灌水总量为6750 m3·hm-2（T5）时，叶片结构紧密度较大，达32.90%；叶片结构疏松度最小，为43.62%；ABA含量最高，达118.87 ng·g-1(鲜重)，GA、IAA、ZR含量均最低；且抗旱基因PbDREB表达量最高，达2.75。从而表明覆膜滴灌、灌水9次、下渗60 cm、年灌水总量为6750 m3·hm-2的灌水方式能够提高叶片结构紧密度、促进ABA合成、提高抗旱基因PbDREB表达量，从而提高叶片的抗旱能力，是甘肃景泰地区梨园的适宜节水灌溉方式 |
| 英文摘要: |
| The experiment of six irrigation models had been conducted in an orchard of 9 year-old ‘Huangguan’ pear. The profile structure, hormone content and the expression of drought resistant gene DREB of leaves were measured. The results showed that there was a positive correlation existed irrigated times or depth and the leaf thickness, cuticle thickness, stockade tissue thickness and CTR under the same irrigated water amount; There was a significant positive correlation between irrigation amount and the expression of the drought resistant gene DREB, T5 (drip irrigation under plastic mulch, irrigated 9 times and each time water move down to 60 cm depth of soil, annual irrigation water at 6750 m3·hm-2) had the high CTR and the lowest SSR. The CTR reached to 32.90% and the SSR reached to 43.62%. It also had high content of the ABA, relatively lowest content of GA, IAA, ZR, and the content of the ABA reached to 118.87 ng·g-1(fresh weight). And it had high expression of the drought resistant gene DREB, the content of DREB expression reached to 2.75. In conclusion, T5 could improve the CTR, promote ABA synthesis, and increase the expression of the drought resistant gene DREB, thus improve the drought resistance of the leaves. There by it should be the suitable water\|saving irrigation model for the pear orchard at Jingtai County in Gansu Province. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| | |