| 范娜,白文斌,彭之东,赵建武.粒用高粱耐盐种质资源鉴选与评价[J].干旱地区农业研究,2018,36(3):72~78 |
| 粒用高粱耐盐种质资源鉴选与评价 |
| Identification and evaluation of salt-tolerant sorghum germplasm resources |
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| DOI：10.7606/j.issn.1000-7601.2018.03.11 |
| 中文关键词: 高粱 耐盐性 鉴选与评价 聚类分析 主成分分析 |
| 英文关键词:grain sorghum salt tolerance identification and evaluation cluster analysis principal component analysis |
| 基金项目:高粱遗传与种质创新山西省重点实验室项目“粒用高粱耐盐种质资源鉴选研究”(2018Q-6)；山西省科技攻关项目“高粱深加工关键技术研究-醋糟在土壤改良中的应用研究”(20150311003-1);山西省农科院科技攻关项目-醋糟在土壤改良中的应用（2018Q-6） |
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| 中文摘要: |
| 以50份高粱亲本材料为基础，采用聚类分析和主成分分析的方法，研究了不同材料高粱种子萌发、幼苗生长和抗盐性。通过相关分析、主成分分析和聚类分析，为高粱抗盐材料筛选和耐盐性研究提供理论依据，为高粱品种耐盐基因开发提供材料。实验结果表明，在150mmol·L-1NaCl胁迫下，高粱亲本材料生长指标低于对照。不同品种降低幅度不同。相对发芽率的数值范围为 0.27%~93.4%，相对发芽势数值范围为 15.15%~97.4%，根长数值范围在0.3~4.5 cm，叶长数值范围为 0.4~2.8cm；盐害系数数值范围0.026~0.794。发芽率与发芽势呈正相关，相关系数为0.98***。根据主成分分析，第一个主要成分是发芽成长因子;第二主成分是生长因子，第三主成分是耐盐因子。通过聚类分析，将30株高粱菌株分为5类：高耐盐，耐盐，中度耐盐，盐敏感性和高盐敏感性。其中3560R，10337B和10612属于盐敏感品系，09305R和67B属于高盐品系。本研究还表明，高粱发芽率，发芽势、芽长、根长、苗高、生长速度、枯萎病指数、发芽率盐害指数、发牙势盐害系数等9项指标可作为高粱耐性的重要指标。 |
| 英文摘要: |
| 50 sorghum parents material were used to study the seed germination, seedling growth and salt tolerance of different sorghum materials, using Cluster Analysis and Principal Component Analysis. By correlation analysis, principal component analysis and cluster analysis, This work provides the theoretical basis for salt-tolerant sorghum selection and salt tolerance research, and to provide material for salt-tolerant gene mining of sorghum varieties. The experimental results showed that, under the stress of 150mmol·L-1 NaCl, the growth index of sorghum parental materials is lower than the control, Different varieties had different reduction levels. The relative germination rate ranged from 0.27 to 93.4%, the relative germination potential ranged from 15.15% to 97.4%, the root length range was from 0.3 to 4.5 cm, the leaf length range was from 0.4 to 2.8 cm, and the salt injury coefficient range was 0.026 to 0.794.. The rate of germination rate is positively correlated with germination rate and germination potential, and the correlation coefficient is 0.98***. According to the principal component analysis, the first principal component is the germination growth factor; the second principal component is the growth factor, and the third principal component is the salt tolerance factor. The cluster analysis divided 30 sorghum strains into five categories: high-salt-tolerant, salt tolerant, moderate-salt-tolerant, salt-sensitive and high-salt-sensitive. Among which 3560R, 10337B and iron 10612 belong to the salt-sensitive category, and 09305R and 67B are high-salt products. In this study, it also showed that sorghum germination rate, germination potential, shoot length, root length, seedling height, growth rate, fusarium wilt index, germination rate salt damage coefficient and germination potential salt damage coefficient can be used as important indexes for sorghum salt tolerance. |
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