| 赵小强,彭云玲,方鹏,武博洋,闫慧萍.不同外源调节物质对低温胁迫下玉米的缓解效应分析[J].干旱地区农业研究,2018,36(3):184~193 |
| 不同外源调节物质对低温胁迫下玉米的缓解效应分析 |
| Mitigation effect analysis of different exogenous regulatory substances on maize under low-temperature stress |
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| DOI：10.7606/j.issn.1000-7601.2018.03.28 |
| 中文关键词: 玉米 低温胁迫，外源脯氨酸 甜菜碱 缓解指数 综合评价 |
| 英文关键词:maize low-temperature stress exogenous Pro Glycine betaine(GB) mitigation index comprehensive evaluation |
| 基金项目:国家自然基金项目(31260330, 31301333); 教育部博士点基金(20126202120001); 甘肃省国际合作项目(1504WKCA009); 甘肃省重大科技专项(17ZD2NA016); 伏羲“青年英才”项目(GAUFX-02Y09) |
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| 中文摘要: |
| 脯氨酸（Pro）和甜菜碱（GB）是一类重要的渗透调节物质，其与低温胁迫下植物的抗逆性密切相关。为了探讨不同浓度的外源Pro或GB对低温胁迫下玉米种子萌发、幼苗性状的影响，综合评价Pro或GB的低温缓解效果。本试验以3份寒敏感自交系为试材，分别在萌发期和苗期10℃低温胁迫下，用5种不同浓度的Pro或GB进行浸种、培养作为试验处理；25℃和10℃下以双蒸水浸种、培养作为正向、负向对照处理CK（+）、CK（-）。结果表明：（1）低温胁迫下，玉米种子的发芽势、发芽率、胚芽长、胚根长、胚芽鲜重和胚根鲜重显著降低；幼苗的相对电导率、丙二醛、脯氨酸及可溶性糖含量显著升高，SOD、POD及CAT活性显著增强。（2）添加外源Pro或GB后，除幼苗相对电导率和丙二醛含量明显降低外，其余5个幼苗性状和6个萌发性状都明显升高。（3）低温胁迫下，6个萌发性状和7个幼苗生理生化性状在自交系间差异显著，在Pro或GB浓度间差异显著，在自交系与浓度互作间差异显著。（4）利用低温缓解指数EI作为综合评价指标，并采用隶属函数法对外源Pro或GB的低温缓解效果进行综合评价表明，萌发期600、800μmol·L-1 Pro;20μmol·L-1 GB的综合低温缓解效果最佳，为0.882或0.647，1000μmol·L-1 Pro或50μmol·L-1 GB的综合缓解效果最小为0.513或0.022；苗期400μmol·L-1 Pro或10μmol·L-1 GB的综合低温缓解效果最佳为0.577或0.500，1000μmol·L-1 Pro或40μmol·L-1 GB的综合低温缓解效果最小仅为0.246或0.289；萌发期和苗期，外源Pro的综合低温缓解效果分别是GB的1.858倍和1.064倍。 |
| 英文摘要: |
| Proline (Pro) and Glycine betaine (GB) are important osmoregulation substances that are associated with cold resistance in plants. To investigate the effect of exogenous Pro and GB on maize germination and seedling traits under low-temperature stress, and to comprehensively evaluate the cold mitigation effect of Pro and GB. Three maize chilling sensitive lines were used with five different concentrations of Pro or GB’s soak or culture treatment under 10℃ stress at germination or seedling stage. Double distilled water soak and culture were used as positive and negative control check (CK(+) and CK(-)) under 25℃ and 10℃ at two stages. It showed that: (1) The germination potential (GP), germination rate (GR), plumule length (PL), radical length (RL), shoot fresh weight (SFW), root fresh weight (RFW) of maize seed significantly decreased, and relative conductivity (RC), malondialdehyde content (MDA), proline content (Pro), soluble sugar content (SS), SOD, POD and CAT activity of seedling significantly increased under low-temperature stress. (2) RC and MDA significantly decreased, and other five traits of seedling and six traits of seed germination significantly increased after adding exogenous Pro and GB under low-temperature stress. (3) The six germination traits and seven physiological and biochemical traits showed significant difference in inbred lines, in the concentrations of Pro and GB, and in the interaction of inbred lines and concentration of Pro and GB. (4) Cold ease index (EI) was used as comprehensive evaluation index, and membership function method was used to comprehensive evaluate the exogenous Pro and GB’s mitigation effect to colde stress. 600, 800μmol·L-1 of Pro and 20μmol·L-1 of GB had the best cold mitigation effect of 0.882 and 0.647, and 1000μmol·L-1 of Pro and 50μmol·L-1 of GB had the worst cold mitigation effect of 0.513 and 0.022 at germination. 400μmol·L-1 of Pro and 10μmol·L-1 of GB had the best cold mitigation effect of 0.577 and 0.500, and 1000μmol·L-1 of Pro and 40μmol·L-1 of GB had the worst cold mitigation effect of 0.246 or 0.289 at seedling stage. The cold mitigation effect of Pro was 1.858 and 1.064 times of GB at germination and seedling stage, respectively. |
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