| 刘建新,刘秀丽,王金成.燕麦幼苗对干旱-低温交叉适应的生理响应及其综合评价[J].干旱地区农业研究,2018,36(4):172~177 |
| 燕麦幼苗对干旱-低温交叉适应的生理响应及其综合评价 |
| Comprehensive evaluation and physiological response of Avena nuda seedlings to drought and low temperature cross adaptation |
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| DOI：10.7606/j.issn.1000-7601.2018.04.25 |
| 中文关键词: 燕麦 干旱-低温交叉适应 生理响应 综合评价 |
| 英文关键词:oat drought and low temperature cross adaptation physiological response comprehensive evaluation |
| 基金项目:甘肃省庆阳市科技计划项目（KZ2014-19） |
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| 中文摘要: |
| 为探讨干旱能否诱导燕麦对低温的交叉适应性，采用20%聚乙二醇6000(PEG)模拟干旱预处理定莜6号幼苗3 d，以未经PEG预处理的幼苗作对照，恢复 2 d 后进行低温(昼/夜温度8℃/5℃)处理，分别在低温处理的0、1、3、5、7天采取幼苗叶片，测定超氧阴离子(O[XZ(180#][XZ)][KG-*3/5]2)、过氧化氢(H2O2)、丙二醛(MDA)、抗坏血酸(ASA)、谷胱甘肽(GSH)、可溶性糖(SS)、可溶性蛋白质(SP)、游离氨基酸(AA)和脯氨酸(Pro)含量及相对含水量(RWC)和超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)活性等14项生理指标及处理7 d后植株株高增量和生物量增量，采用主成分分析和隶属函数综合评价燕麦幼苗对干旱-低温的交叉适应能力。结果表明，与对照相比，干旱预处理显著提高了低温胁迫下燕麦幼苗株高增量和生物量增量，O[XZ(180#]÷[XZ)][KG-*3/5]2和H2O2等14项生理指标值也发生了不同程度改变。对14项生理指标值离差标准化后进行相关性分析表明，不同变量间存在显著相关性。进行主成分分析提取了5个主成分因子，其方差贡献率依次为38.881%、18.219%、11.238%、9.616%和8.809%，累积方差贡献率达86.763%。第1主成分因子对SS、AA、Pro、O[XZ(180#]÷[XZ)][KG-*3/5]2、H2O2、RWC、SOD和APX有较大载荷；第2主成分因子对POD、MDA和GSH有较大载荷；第3主成分因子主要包括CAT和GSH；第4主成分因子主要包括SP和Pro；第5主成分因子主要包括 APX和ASA。隶属函数分析5个主成分因子得分值显示，除第1天外，干旱预处理在整个低温处理期间的综合评价值显著高于对照。研究表明干旱能够诱导燕麦对低温的交叉适应性。 |
| 英文摘要: |
| To explore whether drought could induce oat’s (Avena nuda L.) cross adaptation to low temperature, Dingyou No. 6 seedlings were pretreated with drought condition, simulated by 20% polyethylene glycol 6000 (PEG) for 3 d. After recovering for 2 days, both pretreated and control were treated under low temperature (day/night temperature is 8℃/5℃). 14 physiological indexes of the seedling leaves were determined, including superoxide anion (O[XZ(180#][XZ)][KG-*3/5]2), H2O2, malondialdehyde (MDA), ascorbic acid (ASA), Glutathione (GSH), Soluble sugar (SS), Soluble protein (SP), Amino acid (AA), and proline (Pro) contents, relative water content (RWC) and superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX) activities at 0, 1, 3, 5, 7 days after low temperature treatment, as well as the plant height increment and biomass increment of oat seedlings 7 days after low temperature stress. Principal component analysis and membership function analysis were used to evaluate the drought and low temperature resistance of oat seedlings. Results showed that compared with the control, drought pretreatment significantly enhanced the plant height increment and biomass increment of oat seedlings under low temperature stress, 14 physiological index values of oat seedling leaves, such as O[XZ(180#][XZ)][KG-*3/5]2, H2O2 and so on have also changed in different degree. The correlation analysis of 14 physiological index values after converting standardized deviation showed that there was a significant correlation between different variables. The principal component analysis with 5 principal component factors showed taht the variance contribution rate were 38.881%, 18.219%, 11.238%, 9.616% and 8.809% respectively. The cumulative variance contribution rate was 86.763%. For the primary group of principal components, SS, AA, Pro, O[XZ(180#][XZ)][KG-*3/5]2, H2O2, RWC, SOD and APX were the major contributors, while for the secondary group of principal components, POD, MDA and GSH were the major contributors, for the third group of principal components, CAT and GSH were the major contributors, for the fourth group of principal components, SP and Pro were the major contributors, for the fifth group of principal components，APX and ASA were the major contributors. 5 principal component score values in the subordinate function analysis showed that the comprehensive evaluation values of drought pretreatment were significantly higher than control during almost the whole low temperature treatment except the first day. In conclusion, drought could induce the cross adaptation to low temperature in oat seedlings. |
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