| 朱燕芳,王延秀,胡亚,贾旭梅,郭爱霞.多效唑对水分胁迫下苹果砧木八棱海棠光合及抗氧化酶活性等生理特性的影响[J].干旱地区农业研究,2018,36(4):178~186 |
| 多效唑对水分胁迫下苹果砧木八棱海棠光合及抗氧化酶活性等生理特性的影响 |
| Effects of paclobutrazol on photosynthetic and antioxidant enzyme activities and other physiological characteristics of malus robusta rehd. under water stress |
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| DOI：10.7606/j.issn.1000-7601.2018.04.26 |
| 中文关键词: 水分胁迫 多效唑 八棱海棠 光合特性 抗氧化酶活性 |
| 英文关键词:water stress paclobutrazol Malus robusta Rehd photosynthetic characteristics antioxidant enzyme activities |
| 基金项目:兰州市科技局项目（2015-3-76）；甘肃省自然科学基金项目（2011-2014） |
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| 中文摘要: |
| 以10叶龄八棱海棠（Malus robusta Rehd.）实生苗为试验材料，采用盆栽控水结合外源喷施多效唑（PBZ）的方法，探讨水分胁迫下八棱海棠对不同浓度外源多效唑（0、50、100、150、200 mg·L-1）的响应特性，研究其光合、荧光及抗氧化酶活性的变化。 结果表明：与对照（持续控水胁迫，不喷施任何溶液）相比，外源喷施PBZ有效减缓了水分胁迫下八棱海棠胞间CO2浓度（Ci）、非光化学猝灭系数（NPQ）、过氧化物酶活性（POD）、相对电导率（REC）、脯氨酸（Pro）及丙二醛含量（MDA）的增加程度；并能控制其净光合速率（Pn）、气孔导度（Gs）、蒸腾速率（Tr）、最大光化学效率（Fv/Fm）、实际光化学效率（ΦPSⅡ）、光化学猝灭系数（qP）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）活性的下降；其中对照组及各处理组NPQ分别升高了400.00%、348.28%、212.90%、153.13%、292.86%、266.67%，Pro分别升高了312.50%、246.67%、226.83%、129.55%、166.67%、189.58%，对照组和各处理组Pn分别下降了53.72%、50.00%、35.71%、22.28%、32.78%、31.52%，ΦPSⅡ分别下降了51.22%、48.19%、35.00%、30.33%、41.25%、40.74%，处理组各指标变化程度均低于对照，且100 mg·L-1处理组变化程度低于其他处理组，具有明显的浓度效应。 干旱胁迫下，PBZ能够激活八棱海棠幼苗的防御系统及保护细胞膜系统，从而提高其耐旱性。采用隶属函数法和主成分分析对各项指标进行综合评价结果表明，100 mg·L-1 PBZ处理对缓解八棱海棠的水分胁迫效应最为显著。 |
| 英文摘要: |
| The 10 leaf age seedling of Malus robusta Rehd. was used as a test material, and the pot experiments by controlling water content was applied in combination with spraying exogenous paclobutrazol (PBZ) to study the effects of different concentration exogenous paclobutrazol(0,50,100,150,200 mg·L-1) on Malus robusta Rehd. under water stress, and explore the changes of the photosynthesis, fluorescence and physiological characteristics. The results showed that:compared with the control, exogenous application of PBZ effectively slowed the increasing range of the intercellular CO2 concentration(Ci), non-photochemical quenching coefficient (NPQ), peroxidase activity (POD), electrolyte leakage(REC), Proline(Pro), and malondialdehyde (MDA) content of Malus robusta Rehd. under water stress, and alleviated the decreasing range of net photosynthetic rate(Pn), stomatal conductance(Gs), transpiration rate(Tr), the maximum quantum yield of PSII (Fv/Fm), actual photochemical efficiency(ΦPSⅡ), photochemical quenching coefficient(qP), the activity of catalase(CAT), the activity of superoxide dismutase(SOD). The NPQ in the control group and the treatment groups were increased by 400.00%, 348.28%, 212.90%, 153.13%, 292.86%, 266.67% respectively, the Pro respectively increased by 312.50%, 246.67%, 226.83%, 129.55%, 166.67%, 189.58%, and the Pn respectively decreased by 53.72%, 50.00%, 35.71%, 22.28%, 32.78%, 31.52%, with ΦPSⅡ decreased by 51.22%, 48.19%, 35.00%, 30.33%, 41.25%, 40.74%. Also the change degree of each index in PBZ treatment groups was lower than that in the control group, and the change degree of each index in 100 mg·L-1 PBZ treatment group was lower than that of the other PBZ treatment groups, which exhibited significant concentration-dependent effect. PBZ activate the defense system and protect the cell membrane system of seedlings under water stress, thus improving its drought tolerance The comprehensive evaluation of the indicators using the fuzzy membership analysis and principal component analysis showed that the 100 mg·L-1 PBZ treatment had the most significant effect on the water stress relief of Malus robusta Rehd. |
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