| 张辉,王荷,张蓓蓓,武悦萱,王苗苗.光强对黑麦草萌发生长、叶片叶绿素含量及光系统II的影响[J].干旱地区农业研究,2018,36(4):207~213 |
| 光强对黑麦草萌发生长、叶片叶绿素含量及光系统II的影响 |
| Effects of light intensity on germination, growth, chlorophyll content and photosystem II of leaves in Ryegrass（Lolium Perenne L） |
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| DOI：10.7606/j.issn.1000-7601.2018.04.30 |
| 中文关键词: 黑麦草 光强 萌发生长 叶绿素含量 光系统Ⅱ |
| 英文关键词:ryegrass(Lolium Perenne L） light intensity germination and growth chlorophyii content PSII |
| 基金项目:国家自然科学基金（41601016）；宝鸡市科技计划项目（16RKX1-14）；黄土高原土壤侵蚀与旱地农业国家重点实验室开放基金（K318009902-1403）；陕西省科技厅自然基金（2017JQ4023）；宝鸡文理学院重点项目（ZK2018025） |
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| 中文摘要: |
| 以常见景观植物黑麦草为试验材料，探究6种不同光照强度对水培黑麦草萌发生长、叶片叶绿素含量和光系统II的影响。结果表明：光强为300 μmol·m-2·s-1时水培黑麦草萌发及生长最好，发芽率、发芽势、发芽指数和活力指数分别为93%、23.67%、57.28和211.58，根长、芽长分别为3.04 cm和4.01 cm，该光强下叶片叶绿素含量显著高于其它光强处理（P<0.05），叶绿素a、b及总叶绿素含量分别为0.71、0.27 mg·g-1和0.98 mg·g-1；随着光强的增强，最大荧光（Fm）、PSⅡ的最大光化学效率（Fv/Fm）和光化学性能指数（PIabs）呈现先升高后下降的趋势，300 μmol·m-2·s-1光强下Fm、Fv/Fm和PIabs值都达到最大；叶绿素荧光快速诱导曲线（OJIP）受光强影响较大，以I点差异较显著，对OJIP曲线进行标准化后差异较小；OJIP曲线参数分析表明PSII供体侧在6组光强下无显著差异，随着光强增强，单位反应中心吸收（ABS/RC）、捕获（TRo/RC）和传递（ETo/RC）也随着增强，但单位反应中心热耗散（DIo/RC）减小，PSII受体侧在500 μmol·m-2·s-1条件下电子传递受阻。 |
| 英文摘要: |
| Using common ryegrass（Lolium Perenne L） as a test material, the effects of six different light intensities on germination, growth, chlorophyll content and photosystem II of leaves in ryegrass were studied. The results showed that: (1)When the light intensity was 300 μmol·m-2·s-1, the ryegrass was the best for germination and growth. The germination rate, germination potential, germination index and vigor index were 93%, 23.67%, 57.28 and 211.88, respectively. The root length and shoot length were 3.04 cm and 4.01 cm, respectively; the chlorophyll content of leaves under this light intensity was significantly higher than that of other light intensity treatments (P<0.05), and the chlorophyll a, b and total chlorophyll were 0.71， 0.27 mg·g-1 and 0.98 mg·g-1; (2) With the increase of light intensity, the maximum fluorescence (Fm), the maximum photochemical efficiency of PSII (Fv/Fm) and PIabs showed a trend of increasing first and then decreasing, while the values of Fm, Fv/Fm and PIabs reached the maximum under light intensity 300 mol·m-2·s-1; OJIP was greatly affected by light intensity, with significant difference at point I, and the OJIP curve was normalized with little difference.; (4) OJIP curve parameters showed that there was no significant difference in light intensity of the six donor groups on the PSII, with the increase of light intensity, per reaction center absorption (ABS/RC), capture (TRo/RC) and transfer (ETo/RC) also increases, but the heat dissipation (DIo/RC) per reaction center decreases, and the electron transfer of PSII receptor side is blocked at light intensity 500 μmol·m-2·s-1. |
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