| 张小娟.青海省不同生态区蚕豆根瘤菌16S rDNA分析[J].干旱地区农业研究,2018,36(4):259~263 |
| 青海省不同生态区蚕豆根瘤菌16S rDNA分析 |
| 16S rDNA of rhizobium isolated from faba bean of different ecotopes in Qinghai Province |
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| DOI：10.7606/j.issn.1000-7601.2018.04.37 |
| 中文关键词: 蚕豆 根瘤菌 16S rDNA 系统发育 |
| 英文关键词:faba bean, rhizobium, 16S rDNA, phylogeny |
| 基金项目:青海省科技厅青年基金项目（2014-ZJ-926Q） |
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| 中文摘要: |
| 为了阐明青海省蚕豆根瘤菌的遗传多样性和亲缘关系，同时为发现和获得新的根瘤菌物种提供资源，采集和分离了青海省不同生态区蚕豆主栽品种青蚕14中的根瘤菌，并对其中分离到的6个菌株（CD1~6）进行16S rDNA鉴定分析。将6株蚕豆根瘤菌的全序列结果与NCBI中已报道的序列进行相似性比对，发现其相似度较高，其中供试菌株CD4与KF008225.1相似度最高，达到98%，其余均在95%到96%之间。6株供试菌分属4个菌属，CD1和CD2共属节细菌属，CD3属于分枝杆菌属，CD4和CD5属于快生根瘤菌属，CD6属于中慢生根瘤菌属，表明来自不同地区的蚕豆根瘤菌存在较大差异。聚类分析结果显示，蚕豆根瘤菌的6个菌株分属5个不同系统发育分支，证明青海省不同生态区蚕豆根瘤菌种类多样性较为丰富。 |
| 英文摘要: |
| It has been proved to be benefitial for utilization of faba bean-rhizobium system. To clarify genetic diversity of faba bean rhizobia in Qinghai province and discover new rhizobiums, we isolated rhizobiums from Qingcan14 and used 16S rDNA to analyze the six isolated strains. Blast results showed a high similarity between the six strains and reported sequences. Among them, CD4 showed 98% similarity with KF008225.1, and the remaining strins showed a similarity ranging from 95% to 96%. The six strains belonged to four genus, Arthrobacter (CD1 and CD2), Mycobacterium (CD3), Rhizobium (CD4 and CD5), Mesorhizobium (CD6), which were diverse between ecotopes. The cluster results showed that six strains belonged to five branches, indicating the high genetic diversity of rhizobiums in Qinghai province. |
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