| 赵颖,魏小红,马文静,骆巧娟,宿梅飞.硝普钠(SNP)浸种对渗透胁迫下不同紫花苜蓿种子萌发生理特性的影响[J].干旱地区农业研究,2018,36(6):63~70 |
| 硝普钠(SNP)浸种对渗透胁迫下不同紫花苜蓿种子萌发生理特性的影响 |
| Effects of sodium nitroprusside (SNP)on physiological characteristics of alfalfa seed germination under osmotic stress |
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| DOI：10.7606/j.issn.1000-7601.2018.06.10 |
| 中文关键词: 紫花苜蓿；外源一氧化氮(NO) 干旱胁迫；渗透调节物质；氧化损伤 |
| 英文关键词:alfalfa exogenous nitric oxide drought stress osmoregulation substances oxidative damage |
| 基金项目:国家自然基金项目（31560663）；甘肃省自然基金项目（145RJZA196） |
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| 中文摘要: |
| 以紫花苜蓿阿尔岗金（Medicago sativa ‘Algonquin’）、金皇后（M. sativa ‘Golden empress’）和三得利（M.sativa ‘Sanditi’）为材料，探讨外源一氧化氮(NO)对PEG胁迫下紫花苜蓿种子萌发期丙二醛和渗透调节物质含量及抗氧化酶活性的影响。结果表明：（1）SNP浸种能有效缓解PEG胁迫下紫花苜蓿萌发期MDA含量的积累，在第6 d SNP+PEG与PEG处理相比，阿尔岗金、金皇后和三得利种子MDA含量分别降低了23.11%，21.81%和30.38%。（2）随PEG胁迫时间延长，3个苜蓿品种渗透调节物质含量呈先增后降趋势。SNP+PEG与PEG处理相比，阿尔岗金中脯氨酸、可溶性糖和可溶性蛋白含量分别在处理第4 d,第2 d和第4 d达到最大值，分别提高了13.71%，18.57%和9.23%；金皇后各含量均在第2 d有最大值，分别提高了20.96%，16.84%和5.48%；三得利各含量分别在第4 d,第2 d和第2 d达到最大值，分别提高了17.87%,17.52%和13.40%。（3）3个品种紫花苜蓿SOD、POD、CAT、APX活性表现为先升后降趋势，且均在第4 d活性最大。与PEG处理相比，SNP+PEG处理下三得利SOD、POD活性增加率是阿尔岗金的2.76倍和1.21倍，是金皇后的4.10倍和1.21倍。渗透调节物质含量增加和抗氧化酶活性增强是紫花苜蓿适应干旱的生理基础，外源NO可提高紫花苜蓿对干旱的防御，3个品种紫花苜蓿受外源NO调节强弱顺序为：三得利＞金皇后＞阿尔岗金。 |
| 英文摘要: |
| The aim of this research was to investigate the effects of exogenous nitric oxide on malondialdehyde (MDA), osmoregulation compounds, and antioxidase activity of alfalfa seed germination under polyethylene glycol (PEG) stress. Three alfalfa varieties were selected, Algonquin, Golden empress, and Sanditi. The seeds were placed in Petri dishes with filter paper soaked with PEG for germination. We measured concentrations of malondialdehyde and osmoregulation compounds, and antioxidase activity. Our results showed that SNP+PEG treatment significantly decreased MDA content of the seeds compared to PEG treatment. MDA content in the seeds at the sixth day of germination decreased by 23.11% in Algonquin， 21.81% in Golden empress, and 30.38% in Sanditi. Compared to PEG stress, exogenous application of SNP+PEG increased proline by 13.71% at the fourth day, soluble sugar by 18.57% at the second day, and soluble protein content by 9.23% at the fourth day in Algonquin seeds and they reached the peak values at those time. Those 3 compounds in Golden empress peaked at the second day by 20.96%，16.84%, and 5.48%, respectively and peaked in Sanditi seeds at fourth day by 17.87%, 17.52 %, at the second day, and 13.40% at the second day, respectively. Furthermore, the activities of 4 antioxidases, superoxide dismutase (SOD), guaiacol peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX), increased first and then decreased and peaked at the fourth day. Compared to PEG stress, we noticed that SOD and POD activities of Sanditi seeds were 2.76 and 1.21 times of that of Algonquin, 4.10 and 1.21 times of that of the golden empress, respectively. Our results suggested that, under osmotic stress generated by PEG, the enhancement of exogenous SNP on alfalfa was mainly embodied in the osmoregulation substances and antioxidase activity. The sensitivity of Sanditi to NO impact was greater than that of Golden empress and Algonquin. |
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