| 王军娥,景维坤,王佳敏,亢秀萍.4种不同石竹属植物的抗旱性分析[J].干旱地区农业研究,2018,36(6):71~76 |
| 4种不同石竹属植物的抗旱性分析 |
| Analysis of drought tolerance of four different Dianthus L. |
| |
| DOI：10.7606/j.issn.1000-7601.2018.06.11 |
| 中文关键词: 石竹属植物 干旱胁迫 生理指标 隶属函数法 实时荧光定量PCR |
| 英文关键词:Dianthus L. plant drought stress physiological index subordinate function real-time PCR |
| 基金项目:山西省应用基础研究计划项目（201601D021126）；山西农业大学引进人才博士科研启动项目(2013YJ21) |
|
| 摘要点击次数: 1260 |
| 全文下载次数: 485 |
| 中文摘要: |
| 以瞿麦（Dianthus superbus L.）、常夏石竹（Dianthus plumarius）、中国石竹（Dianthus chinensis L.）和须苞石竹（Dianthus barbatus L.）4种不同石竹属植物幼苗为试材，采用自然干旱胁迫法对其进行处理，测定不同干旱胁迫下石竹幼苗的形态和生理指标，并用隶属函数法对4种石竹属植物的抗旱性进行综合评价，旨在筛选出适合山西省栽培和利用的石竹属抗旱性种质资源。研究结果表明：随着干旱胁迫程度的加剧，4种石竹属植物幼苗的土壤含水量和叶片相对含水量均下降，处理第12 d时各处理组叶片相对含水量和对照相比分别下降64.14%、59.44%、80.84%和78.58%；丙二醛（MDA）含量第10 d时处理组和对照组相比上升451.61%、353.45%、724.32%和647.95%；处理组和对照组相比超氧化物歧化酶（SOD）活性在第8 d分别上升77.58%、90.68%、29.05%和81.52%；脯氨酸（Pro）含量持续上升，叶绿素（Chl）、可溶性蛋白质含量以及超氧化物歧化酶（SOD）的活性呈先上升后下降的趋势；干旱胁迫处理第10 d时，DtSOD和DtPOD基因在常夏石竹和瞿麦中表达水平较高，分别为对照组的2.5倍和2.25倍。利用隶属函数法分析得出4种不同石竹属植物的抗旱性从强到弱依次为：常夏石竹＞瞿麦＞须苞石竹＞中国石竹。 |
| 英文摘要: |
| The seedlings of four different Dianthus plants (Dianthus superbus L., Dianthus plumarius, Dianthus chinensis L.,and Dianthus barbatus L.) were used as test plants to evaluate their drought tolerance under natural drought stress.Morphological and physiological indexes related to the drought tolerance were measured, and the comprehensive evaluation method of drought tolerance capacity was evaluated using subordinate function value analysis on four different Dianthus plants.The results indicated that soil water content and relative water content of leaves of Dianthus plants were decreased with increasing severity of drought stress. Compared with the control, after drought stress of 12 d, the relative water content of leaves decreased by 64.14%、59.44%、80.84%、and 78.58%in all 4 studied plants,respectively.The content of malondialdehyde (MDA) increased 451.61%、353.44%、724.32%, and 647.95% after 10 d drought stress. The activity of superoxide dismutase (SOD) increased 77.58%、90.68%、29.05%,and 81.52% at day 8. The content of proline (Pro) increased while the content of chlorophyll (Chl) and the activity of superoxidedismutase (SOD) went up first and then decreased. At day 10, DtSOD and DtPOD genes showed rising trend in Dianthus plumarius and Dianthus superbus L. increased 2.5 and 2.25 times over that of control. The order of the drought tolerance of four Dianthus plants was Dianthus plumarius>Dianthus superbus>Dianthus barbatus>Dianthus chinensis. |
| 查看全文 查看/发表评论 下载PDF阅读器 |