程亮,王信,郭青云.青藏高原不同生境土壤细菌群落结构特征及其与环境的关系[J].干旱地区农业研究,2019,37(1):18~26
青藏高原不同生境土壤细菌群落结构特征及其与环境的关系
Relationship of soil bacterial community composition from different ecosystems on Qinghai-Tibet Plateau with environment factors
  
DOI：10.7606/j.issn.1000-7601.2019.01.03
中文关键词:  细菌群落  高通量测序  微生物-环境关系  青藏高原
英文关键词:bacterial community  high-throughput sequencing  microorganism-environment relationships  Qinghai-Tibet Plateau
基金项目:青海省科技计划项目基础研究计划(2015-ZJ-705)；青海大学中青年科研基金项目(2015-QNY-2)
作者单位
程亮 青海大学农林科学院植物保护研究所，青海 西宁 810016；青海大学省部共建三江源生态与高原农牧业国家重点实验室，青海 西宁 810016；青海省农业有害生物综合治理重点实验室，青海 西宁 810016 
王信 青海大学农林科学院土壤肥料研究所，青海 西宁 810016；青海大学省部共建三江源生态与高原农牧业国家重点实验室，青海 西宁 810016 
郭青云 青海大学农林科学院植物保护研究所，青海 西宁 810016；青海大学省部共建三江源生态与高原农牧业国家重点实验室，青海 西宁 810016；青海省农业有害生物综合治理重点实验室，青海 西宁 810016 
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中文摘要:
      为了解土壤微生物在青藏高原的分布状况及环境依赖特点，对青藏高原5种不同类型的土壤微生物群落结构、组成及其与环境关系进行了研究。通过高通量测序技术研究青藏高原不同生境土壤细菌的组成和多样性，共获得1 463 157条16S rRNA基因序列。各样品中可操作分类单元(Operational taxonomic units, OTUs)在4 344~5 764 之间。土壤样品中细菌以变形菌门(35.5%)、放线菌门(20.4%)、酸杆菌门(12.5%)、拟杆菌门(7.3%)、绿弯菌门(6.1%)和厚壁菌门(5.3%)为主，其中变形菌门最为丰富。在变形菌门中，α-变形菌纲是第一大纲，占有45.9%的OTUs，其后是γ-变形菌纲和β-变形菌纲。Person相关性分析和RDA分析表明，土壤含水量、有机碳含量与钾含量对细菌群落分布起主要作用。
英文摘要:
      To evaluate the distribution and the environmental dependence of soil microbes in arid land, we studied the composition and diversity of microbial communities in soils from different ecosystems on Qinghai-Tibet Plateau along with relevant environmental factors. The composition and diversity of Qinghai-Tibet Plateau soil bacteria were investigated by the high-throughput sequencing. Totally, 1 463 157 partial 16S rRNA gene sequences were obtained. These sequences revealed great amount of operational taxonomic units(OTUs), that is, 4 344~5 764 OTUs in soil samples. There were mainly Proteobacteria(35.5%), Actinobacteria(20.4%), Acidobacteria(12.5%), Bacteroidetes(7.3%), Chloroflexi(6.1%), and Firmicutes(5.3%), which had the largest number of Proteobacteria. And Alphaproteobacteria with 45.9% of all OTUs was the most abundant class of Proteobacteria, followed by Gammaproteobacteria and Betaproteobacteria of this phylum. Pearson correlation and redundancy analysis revealed that soil moisture, total organic carbon, and soil potassium content had significant effect on soil bacterial community distribution.
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