柳燕兰,郭贤仕,马明生,姜小凤.干旱复水对春玉米幼苗生长和生理特性的影响及其根源ABA调控效应[J].干旱地区农业研究,2019,37(1):187~193
干旱复水对春玉米幼苗生长和生理特性的影响及其根源ABA调控效应
Effects of drought and rewatering on growth and physiological characteristics of maize seedlings and regulation of root-sourced ABA
  
DOI:10.7606/j.issn.1000-7601.2019.01.26
中文关键词:  春玉米;干旱  复水;根源ABA;生长;光合特性;生理特性
英文关键词:spring maize  drought  rewatering  root-sourced ABA  growth  photosynthetic characteristics  physiological characteristics
基金项目:国家自然科学基金(31460323); 国家科技支撑计划(2015BAD22B01-05); 甘肃省农业科学院农业科技创新专项(2017GAAS27)
作者单位
柳燕兰 Institute of Dryland Farming, Gansu Academy of Agricultural Sciences
Key Laboratory of Northwest Drought-resistant Crop Farming and Cultivation, Ministry of Agriculture, People’s Republic of China
Key Laboratory of Efficient Water Utilization in Dryland Farming of Gansu Province, Lanzhou, Gansu 730070, China 
郭贤仕 Institute of Dryland Farming, Gansu Academy of Agricultural Sciences
Key Laboratory of Northwest Drought-resistant Crop Farming and Cultivation, Ministry of Agriculture, People’s Republic of China
Key Laboratory of Efficient Water Utilization in Dryland Farming of Gansu Province, Lanzhou, Gansu 730070, China 
马明生 Institute of Dryland Farming, Gansu Academy of Agricultural Sciences
Key Laboratory of Northwest Drought-resistant Crop Farming and Cultivation, Ministry of Agriculture, People’s Republic of China
Key Laboratory of Efficient Water Utilization in Dryland Farming of Gansu Province, Lanzhou, Gansu 730070, China 
姜小凤 Institute of Dryland Farming, Gansu Academy of Agricultural Sciences
Key Laboratory of Northwest Drought-resistant Crop Farming and Cultivation, Ministry of Agriculture, People’s Republic of China
Key Laboratory of Efficient Water Utilization in Dryland Farming of Gansu Province, Lanzhou, Gansu 730070, China 
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中文摘要:
      为了探明根源ABA对春玉米干旱后复水补偿生长的调控效应,采用盆栽试验以春玉米酒单2号为材料,设CK(正常供水,保持最大持水量的75%~80%)、MS(中度干旱,保持最大持水量的50%~55%)、SS(重度干旱,保持最大持水量的35%~40%)、MS-CK(中度干旱再复水)、SS-CK(重度干旱再复水)等5个处理,研究苗期干旱及复水条件下春玉米生物量积累、光合特性、抗氧化酶活性、根源ABA含量的变化。结果表明:重度干旱胁迫具有较高的根源ABA含量,诱导叶片抗氧化酶SOD、POD、CAT活性显著提高,增幅分别为85.48%、72.67%、22.58%,并导致其叶片气孔导度(Gs)和光合速率(Pn)降低51.56%、12.16%,同时最大电子传递速率(Jmax)、最大羧化速率(VCmax)、磷酸丙糖利用率(TPU)及RuBP羧化酶的羧化效率(CE)降低,使其单株生物量较正常供水降低46.56%;复水后,较高的根源ABA含量影响其细胞膜修复和光合功能恢复的速度,使单株生物量较未复水重度干旱胁迫增加90.36%,较正常供水增加1.73%,表现出补偿效应。中度干旱胁迫具有相对较低的根源ABA含量,诱导叶片抗氧化酶SOD、POD、CAT活性提高幅度较小,增幅分别为64.72%、41.72%、11.62%,并使Gs和Pn分别降低15.01%和6.42%,同时JmaxVCmax、TPU及CE稍有降低,其单株生物量较正常供水仅降低13.64%;复水后,相对较低的根源ABA含量最终使单株生物量较未复水的中度干旱胁迫增加30.71%,较正常供水增加12.88%,表现出超补偿效应。综上,干旱胁迫产生的根源ABA主要对旱后复水春玉米光能吸收、光合碳同化及抗氧化保护功能起积极调节作用。
英文摘要:
      To reveal the regulation effect of root-sourced ABA to compensatory growth of spring maize after rehydration. Spring maize Jiudan 2 grown in pots experiment in greenhouse was subjected to five different treatments(well-watered and maintaining 75%~80% of maximum water holding capacity(CK)、moderately stressed and maintaining 50%~55% of maximum water holding capacity(MS)、severely stressed and maintaining 35%~40% of maximum water holding capacity(SS)、rehydration after moderately stressed (MS-CK)、rehydration afterseverely stressed(SS-CK)).The maize biomass, photosynthetic characteristics, root-sourced ABA concentrations and antioxidant enzyme activity were measured. The results showed that severe drought stress resulted in higher root-sourced ABA content of 80.15%, and significantly increased the activity of antioxidant enzymes SOD, POD, and CAT, by 85.48%, 72.67%, and 22.58%, respectively, but resulted in significant decrease of leaf stomatal conductance (Gs) and photosynthetic rate (Pn) by 51.56% and 12.16%, while the maximum Jmax, VCmax, TPU, and carboxylation efficiency (CE) of RuBP carboxylase decreased, which resulted in 46.56% decrease in the biomass per plant compared with normal water supply. After rewatering, the higher ABA content affected the recovery rate of cell membrane repairing and photosynthesis. Therefore, the biomass per plant increased by 90.36% compared with severe drought stress and increased by 1.73% compared with normal water supply, showing a compensatory effect. Moderate drought stress had a relatively lower ABA content of 23.53%, which resulted in increase of antioxidant enzymes SOD, POD, and CAT activity in leaves, by 64.72%, 41.72%, and 11.62%, respectively. That made the decrease in Gs and Pn relatively smaller by 15.01% and 6.42%, while Jmax, VCmax, TPU, and CE of RuBP carboxylase decreased less, which reduced the biomass per plant and decreased by only 13.64% compared to normal water supply. After rewatering, the relatively low ABA content in roots eventually increased the biomass per plant by 30.71% compared with moderate drought stress and increased by 12.88% compared with normal water supply showing a super-compensatory effect. In conclusion, the root-sourced ABA caused by drought stress plays a positive regulatory role in light absorption, photosynthetic carbon assimilation, and antioxidant protection on spring maize.
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