李波,邬婷婷.NaN3诱变和盐碱胁迫对苜蓿愈伤组织生长和生理特性的影响[J].干旱地区农业研究,2019,37(2):130~135
NaN3诱变和盐碱胁迫对苜蓿愈伤组织生长和生理特性的影响
Effects of NaN3 mutagenesis and saline-alkali stress on growth and physiological characteristics of alfalfa callus
  
DOI:10.7606/j.issn.1000-7601.2019.02.19
中文关键词:  苜蓿  愈伤组织  NaN3诱变  盐碱胁迫  存活率  生物量  生理特性
英文关键词:alfalfa  callus  NaN3 mutagenesis  saline alkali stress  survival rate  biomass  physiology characteristics
基金项目:黑龙江省省属高等学校基本科研业务费科研项目(135209267);黑龙江省应用技术研究与开发计划重大项目(GA15B105-5)
作者单位
李波 College of AgricultureForestry and Life SciencesHeilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold AreasQiqihar University, Qiqihar,Heilongjiang 161006, China 
邬婷婷 College of AgricultureForestry and Life SciencesHeilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold AreasQiqihar University, Qiqihar,Heilongjiang 161006, China 
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中文摘要:
      为了解NaN3诱变和盐碱胁迫对苜蓿愈伤组织生长和生理特性的影响,利用叠氮化钠(NaN3)诱变和盐碱(NaCl、NaHCO3、Na2CO3)胁迫处理“中苜1号”苜蓿愈伤组织,测定相关生长和生理指标。结果表明:随着NaN3和盐碱浓度的增加,愈伤组织的存活率降低,在NaN3浓度为3.0 mmol·L-1(处理8 d),愈伤组织存活率为54.55%,此NaN3浓度和处理时间为NaN3诱变半致死浓度;在200 mmol·L-1盐碱浓度胁迫下(胁迫12 d),愈伤组织存活率低于5%,此盐碱浓度和胁迫时间为各盐碱致死浓度;除低浓度NaCl胁迫组外,随着NaN3和盐碱胁迫浓度的增加,愈伤组织生物量降低,NaN3降低范围为60.85%~81.88%,NaCl胁迫组降低了13.90%~65.63%,NaHCO3胁迫组降低了21.71%~67.37%,Na2CO3胁迫组降低了61.54%~73.20%,盐碱对愈伤组织生物量的影响为NaCl>NaHCO3>Na2CO3。经NaN3诱变和盐碱胁迫,愈伤组织可溶性蛋白、可溶性糖和脯氨酸含量显著增加,MDA含量和电导率降低,NaN3、NaN3+NaCl、NaN3+Na2CO3、NaN3+ NaHCO3各处理可溶性蛋白含量分别增加了47.07%、53.57%、22.31%、47.69%,可溶性糖含量分别增加了24.79%、162.86%、58.36%、109.54%,脯氨酸含量分别增加了47.78%、162.86%、80.00%、107.25%,MDA含量分别降低了2.35%、11.17%、3.64%、5.54%,相对电导率分别降低了15.13%、17.96%、16.79%、17.28%。NaN3诱变促进愈伤组织渗透调节物质积累和降低膜的透性。
英文摘要:
      In order to decipher the effect of NaN3 mutagenesis and saline-alkali stress on the growth and physiological characteristics of alfalfa callus, we conducted an experiment that the growth and physiological indexes of alfalfa callus, “Zhongmu No.1”were treated by NaN3 and saline-alkali (NaCl, NaHCO3, and Na2CO3) stresses. The results showed that increasing NaN3 and salt concentration decreased the survival rate of callus. When the concentration of NaN3 was at 3 mmol·L-1, 8 d treatment decreased the survival rate of callus to 54.55%. This treatment combination is NaN3 mutagenic semi-lethal conditions. The survival rate of callus was lower than 5% at 200 mmol·L-1of NaN3 after 12 d treatment that is the lethal concentration of each salt and stress time. In addition to the low concentration of NaCl stress group, the biomass of the callus decreased with the increase of NaN3 and salt stress concentration, NaN3 treatment decreased the biomass in a range of 60.85% to 81.88%, the NaCl stress group decreased that by 13.90%~65.63%, the NaHCO3 stress group decreased that by 21.71%~67.37%, and the Na2CO3 stress group decreased that by 61.54%~73.20%. The effect of salt and alkali on the biomass of alfalfa callus ranked in this order: NaCl> NaHCO3> Na2CO3. The content of soluble protein, soluble sugar and proline in the callus increased significantly, and the content of MDA and electrical conductivity decreased by NaN3 mutation and salt stress. The soluble protein content in treatments of NaN3, NaN3+NaCl, NaN3+ Na2CO3 and NaN3+ NaHCO3 increased by 47.07%, 53.57%, 22.31%, and 47.69%, respectively. The soluble sugar content increased by 24.79%, 162.86%, 58.36% and 109.54%, respectively.The proline content increased by 47.78%, 162.86%, 80.00%, and 107.25%, respectively. The MDA content decreased by 2.35%, 11.17%, 3.64%, 5.54%, respectively. The relative conductivity decreased by 15.13%, 17.96%, 16.79% and 17.28% in all treatments, respectively. The NaN3 induced the accumulation of osmotic substances in the callus and decreased the permeability of membrane.
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