陈大为,薛应钰,沈志彦,毛维兴,张树武,徐秉良.生防放线菌ZZ-9耐药菌株的微波诱变选育[J].干旱地区农业研究,2019,37(2):260~265
生防放线菌ZZ-9耐药菌株的微波诱变选育
Screening of chemical-resistant Streptomyces ZZ-9 after microwave mutagenesis
  
DOI:10.7606/j.issn.1000-7601.2019.02.37
中文关键词:  微波诱变选育  耐药菌株  放线菌ZZ-9  协同作用
英文关键词:screening of microwave mutagenesis  resistance strain  streptomyces ZZ-9  synergistic effect
基金项目:国家重点研发计划(2016YFD0201100);甘肃省高等学校科研项目(2017A-028)
作者单位
陈大为 College of Plant Protection, Gansu Agricultural University and Bio-control Engineering Laboratory of Crop Diseases and Pests, Lanzhou, Gansu 730070, China 
薛应钰 College of Plant Protection, Gansu Agricultural University and Bio-control Engineering Laboratory of Crop Diseases and Pests, Lanzhou, Gansu 730070, China 
沈志彦 College of Plant Protection, Gansu Agricultural University and Bio-control Engineering Laboratory of Crop Diseases and Pests, Lanzhou, Gansu 730070, China 
毛维兴 College of Plant Protection, Gansu Agricultural University and Bio-control Engineering Laboratory of Crop Diseases and Pests, Lanzhou, Gansu 730070, China 
张树武 College of Plant Protection, Gansu Agricultural University and Bio-control Engineering Laboratory of Crop Diseases and Pests, Lanzhou, Gansu 730070, China 
徐秉良 College of Plant Protection, Gansu Agricultural University and Bio-control Engineering Laboratory of Crop Diseases and Pests, Lanzhou, Gansu 730070, China 
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中文摘要:
      为了获得耐甲基硫菌灵的菌株, 采用微波诱变法对放线菌ZZ-9菌株进行0~75 s的处理,筛选出在甲基硫菌灵致死浓度下仍能较好生长的突变菌株,并测定其对苹果树腐烂病菌拮抗性和传代稳定性的影响,选育出性能优良的耐药菌株,与甲基硫菌灵协同作用进行防治苹果树腐烂病试验。结果表明,在微波辐照15~60 s条件下,获得4株耐70 μg·mL-1甲基硫菌灵的突变菌株ZZ-9A、ZZ-9B、ZZ-9C和ZZ-9D。通过对4株突变菌株进行抑菌率和传代稳定性测定,筛选出一株抑菌率较好且传代稳定的突变菌株ZZ-9B。菌株ZZ-9B与70 μg·mL-1甲基硫菌灵协同作用测定表明,当菌株ZZ-9B与甲基硫菌灵配比为8∶2时,在离体枝条上对苹果树腐烂病的协同防效为89.42%,明显高于单独使用突变菌株和甲基硫菌灵的防效。因此,在使用菌株ZZ-9B来防治苹果树腐烂病时,添加少量的甲基硫菌灵,可显著提高防效。
英文摘要:
      In order to obtain thiophanate-methyl resistant mutant strains of Streptomyces ZZ-9, the original strain Streptomyces ZZ-9 with low resistance to thiophanate-methyl was mutated using microwave for 0 to 75 s, which could grow well under the lethal concentration of thiophanate-methyl. After genetic stability test of resistance and antagonism to Cytospora sp., ideal resistant strains were screened out. The combination of resistant strains and thiophanate-methyl was used to treat Valsa canker on apple trees. The results showed that with a mutation time of 15 to 60 s, 4 stains with chemicals-resistance capability named ZZ-9A, ZZ-9B, ZZ-9C and ZZ-9D were obtained. After measuring the inhibition rate and culturing several generations, a strain named ZZ-9B with relatively stable hereditary and better inhibition rate was screened out. The synergistic effect of the strain ZZ-9B and 70 μg·mL-1 of thiophanate-methyl showed a 89.42% effectiveness on the apple branches, which was significantly higher than sole use of stain ZZ-9B or thiophanate-methyl. The optimal ratio of the strain ZZ-9B to thiophanate-methyl was 8∶2. It is concluded that small amount of thiophanate-methyl with the strain ZZ-9B can significantly improve the biocontrol efficiency of Valsa canker on apple tree.
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