| 范惠玲,刘秦,白生文,罗天,朱雪峰,缪纯庆.26份芸芥种质的耐盐性评价[J].干旱地区农业研究,2019,37(3):15~22 |
| 26份芸芥种质的耐盐性评价 |
| Evaluation of salt tolerance of 26 Eruca sativa Mill. |
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| DOI：10.7606/j.issn.1000-7601.2019.03.02 |
| 中文关键词: 芸芥 耐盐性 生理特性 隶属函数法 |
| 英文关键词:Eruca sativa salt tolerance physiological characteristics subordinate function value method |
| 基金项目:甘肃省高等学校科研项目(2017A-083)；甘肃省河西走廊特色资源利用重点实验室面上项目 (XZ1804)；2018 年度甘肃省"十三五"教育科学规划高校与职业院校一般课题（GS［2018］GHBBK157）；河西学院第九批大学生科研创新扶持项目（白芥不同生育期耐混合盐碱胁迫能力分析）；甘肃省现代农业产业技术体系建设专项资金 |
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| 中文摘要: |
| 为了明确26份芸芥种质的耐盐性差异，本试验采用6个不同浓度盐溶液，包括0, 0.2%, 0.4%, 0.6%, 0.8% 和 1.0% NaCl处理芽期芸芥，测定了发芽率、胚根长等指标；筛选出0.6%、0.8% NaCl溶液处理苗期芸芥，测定了幼苗生长及耐盐生理相关指标，并采用模糊数学隶属函数法评价不同芸芥材料的耐盐性。 结果表明：(1) 0.6%盐胁迫所造成的芸芥萌发抑制率接近50%，0.8%盐胁迫基本造成了生长的停滞(发芽率8%)，因此认为这两个浓度是盐胁迫抑制芸芥生长的阈值。(2) 随着盐浓度的增加，正常种苗率变小，其均值变化为57.62%～34.45%；苗高、根长、根鲜重、苗鲜重均下降，其中各指标的变幅分别为0.99～0.77 cm，0.69～0.44 cm，0.0034～0.0023 g，0.0244～0.0176 g。(3) 根据隶属函数总平均值大小将参试材料进行耐盐性分类：耐盐性较强的材料（0.6133≤隶属值总平均值≤0.7261），包括08武芸3-1等；耐盐性中等的材料（0.4274≤隶属值总平均值≤0.5911），包括临洮芸芥等；耐盐性较弱的材料（0.3894≤隶属值总平均值≤0.0280），包括13芸芥68-3等。(4) 耐盐材料中游离脯氨酸和可溶性糖含量均显著高于耐盐性较差的材料。总之，26份芸芥种质材料对NaCl溶液胁迫的耐受性存在明显差异，通过模糊数学隶属函数法综合苗期各指标筛选出的耐盐材料，可进一步用于耐盐育种与耐盐基因挖掘。 |
| 英文摘要: |
| To assess the salt tolerance of 26 Eruca sativa Mill., 6 concentration of NaCl solution were used，including 0, 0.2%, 0.4%, 0.6%, 0.8% and 1.0%, to treat Eruca sativa at germination stage, and the germination rate, radicles length were measured. 0.6% and 0.8% NaCl solution were selected to treat the seedling of Eruca sativa, the physiological indexes of seedling growth and salt tolerance were detemined, and all resources were sorted out using the method of membership function. Results showed that: (1) Tests on seed germination indicated that stress of 0.6% NaCl had an inhabitation of 50% nearly, salt stress of 0.8% NaCl basically caused growth stagnation， therefore, these two concentrations were the thresholds of salt stress inhibiting the growth of Eruca sativa; (2) Survival seedlings, shoot height, root length, fresh weight of shoot and root were all decreased with the increase of salt concentration, the mean were 57.62%～34.45%, 0.99～0.77 cm, 0.69～0.44 cm, 0.0034～0.0023 g, 0.0244～0.0176 g, respectively; (3) Based on the membership function on the means of parameters at seedling stages for each growth level, 26 materials were classified into three types: high salt tolerance, including 08 wuyun 3-1, etc (0.6133≤the verage of membership function values≤0.7261); salt tolerance, including Lintao yunjie, etc (0.4274≤the verage of membership function values≤0.5911); low salt tolerance, including 13 yunjie 76-4,etc (0.0280≤the verage of membership function values≤0.3894); (4) The contents of free proline and soluble sugar in high salt tolerant meaterials were significantly higher than those in low salt tolerant materials. In conclusion, significant difference in salt tolerance of 26 Eruca sativa were revealed, the salt tolerant materials screened by method of membership function of fuzzy mathematics could be further used in salt-tolerant breeding and gene mining. |
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