岳凯,魏小红,刘文瑜,韩厅,辛夏青,赵颖.PEG胁迫下不同品系藜麦抗旱性评价[J].干旱地区农业研究,2019,37(3):52~59
PEG胁迫下不同品系藜麦抗旱性评价
Evaluation of drought tolerance of various quinoa species under PEG stress
  
DOI:10.7606/j.issn.1000-7601.2019.03.06
中文关键词:  藜麦品系  PEG胁迫  生长  抗逆生理生化指标  光合指标
英文关键词:quinoa  PEG stress  growth status  physiological and biochemical indexes of stress resistance  photosynthetic indexes
基金项目:国家自然基金项目(31560663);甘肃省农业科学院农业科技创新专项计划(2017GAAS66)
作者单位
岳凯 College of Life Science and TechnologyGansu Agricultural UniversityLanzhou, Gansu 730070China 
魏小红 College of Life Science and TechnologyGansu Agricultural UniversityLanzhou, Gansu 730070China 
刘文瑜 Gansu Academy of Agricultural SciencesLanzhou, Gansu 730070China 
韩厅 College of Life Science and TechnologyGansu Agricultural UniversityLanzhou, Gansu 730070China 
辛夏青 College of Life Science and TechnologyGansu Agricultural UniversityLanzhou, Gansu 730070China 
赵颖 College of Life Science and TechnologyGansu Agricultural UniversityLanzhou, Gansu 730070China 
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中文摘要:
      利用不同浓度PEG溶液模拟干旱胁迫,研究5种品系藜麦幼苗的形态、生理生化及光合特性,并对其进行耐旱性评价。结果表明:15%PEG处理下各品系藜麦株高增量、叶面积及生物量显著(P<0.05)低于对照,其中株高增量、叶面积、生物量下降幅度最小的品系分别是NK1、NK2和NK5,分别比对照下降了44.38%、25.39%和48.23%;随着干旱胁迫加剧, 各藜麦品系叶片内相对含水量显著(P<0.05)下降, 叶片的质膜透性、丙二醛(MDA)含量、脯氨酸(Pro)含量上升,15%PEG胁迫下NK2和NK3的Pro含量分别是对照的2.69和1.93倍, 超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性均先升后降,SOD、CAT和APX活性在5%PEG处理下达到最大值, 而POD活性在10%PEG处理下达到最大; 随干旱胁迫增强,5种品系藜麦幼苗的净光合速率(Pn)、蒸腾速率(Tr)和气孔导度(Gs)降低,胞间CO2浓度(Ci)先降后升,叶绿素(Chl)先升后降,其中NK5品系Pn下降幅度最小,比对照下降了51.15%。运用隶属函数法对藜麦抗旱能力进行综合评定,不同藜麦品系耐旱性为NK5>NK1>NK2>NK4>NK3。
英文摘要:
      In this study,we used various concentrations of PEG solution to simulate drought stress to investigate the morphological, physiological, biochemical,and photosynthetic characteristics of 5 quinoa species, and evaluated their drought tolerance. The results showed that: (1) 15% PEG treatment significantly impacted the height increase rate, leaf area, and biomass of all species over the control(P<0.05).The species with the lowest increase rate of plant height, leaf area, and biomass were NK1, NK2, and NK5, which decreased by 44.38%, 25.39%, and 48.23% compared with the control, respectively.(2) With increasing drought stress, the relative water content in leaves of each quinoa specie decreased significantly(P<0.05), the leaf membrane permeability, malondialdehyde (MDA), and proline (Pro) contents were increased, Pro in NK2 and NK3 were 2.69 and 1.93 times of that in the control under 15%PEG treatment, respectively. The activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbic acid peroxidase (APX) were all initially rose and then declined.SOD, CAT,and APX activities reached the maximum at 5%PEG concentration, while POD activity reached the maximum at 10% concentration treatment.(3) With increasing drought stress, net photosynthetic rate (Pn), transpiration rate (Tr), and stomatal conductance (Gs) decreased. The concentration of intercellular CO2(Ci) decreased first and then increased but chlorophyll (Chl) initially increased and then decreased later. Among all species,the decrease of NK5 Pn was the lowest, which was 51.15% lower than that of the control. In conclusion, the drought tolerance of quinoa was evaluated by the membership function method and the drought tolerance of different quinoa varieties ranked as NK5>NK1>NK2>NK4>NK3.
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