孙牧笛,胡丽杰,李文学,闫思远,张众,顾沛雯.苦豆子内生真菌诱导子促进宿主生物碱合成积累的生理防卫反应[J].干旱地区农业研究,2019,37(3):60~65
苦豆子内生真菌诱导子促进宿主生物碱合成积累的生理防卫反应
Physiological defense response of promoting synthesis and accumulation of host alkaloid by endophytic fungal elicitors in Sophora alopecuroides L.
  
DOI:10.7606/j.issn.1000-7601.2019.03.07
中文关键词:  苦豆子;内生真菌诱导子  氧化苦参碱  防御酶活性;组培苗
英文关键词:Sophora alopecuroides L.  endogenous fungal elicitors  oxymatrine  defense enzyme activity  tissue culture seedling
基金项目:国家自然科学基金项目(31260452;31460484)
作者单位
孙牧笛 Agricultural College, Ningxia University, Yinchuan, Ningxia 750021, China 
胡丽杰 Agricultural College, Ningxia University, Yinchuan, Ningxia 750021, China 
李文学 Agricultural College, Ningxia University, Yinchuan, Ningxia 750021, China 
闫思远 Agricultural College, Ningxia University, Yinchuan, Ningxia 750021, China 
张众 Wine College, Ningxia University, Yinchuan, Ningxia 750021, China 
顾沛雯 Agricultural College, Ningxia University, Yinchuan, Ningxia 750021, China 
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中文摘要:
      分别以4株苦豆子内生真菌菌液浓缩物为诱导子,研究不同诱导时间下,各诱导子对苦豆子组培苗中氧化苦参碱(OMA)含量以及防御酶苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)和过氧化氢酶(CAT)活性的影响。结果显示:4种苦豆子内生真菌的菌液浓缩物均可提高宿主中OMA的积累,其中诱导效果最佳的是内生真菌诱导子XKYKDF40,在0~15 d诱导期间宿主中OMA含量持续增长且始终高于对照,在诱导第15 d达到最高,为3.616 mg·g-1,是同时期对照组的2.09倍。4种内生真菌诱导子还可诱发宿主中生物碱合成关键酶PAL和3种抗氧化酶(POD、APX和CAT)活性升高。诱导子XKYKDF40激发PAL活性的作用最佳,在诱导第6 d PAL活性达最大值,为61.92 U·g-1,是同时期对照组的1.42倍;与其他诱导子相比,诱导子XKZKDF27能够明显诱发POD的活性,在诱导第12 d达到最大值,为62.30 U·mg-1,是同时期对照组的2.70倍;诱导子XKZKDF11可诱导宿主中的APX和CAT活性大幅度提高,在诱导第12 d酶活性达到最高,分别为0.5538 U·mg-1和19.82 U·mg-1,是同时期对照组的4.97倍和3.00倍。结果表明,苦豆子内生真菌诱导子的加入不仅激活了宿主组培苗的防御性应答反应,还促进了OMA的合成。
英文摘要:
      Four species of endophytic fungi were isolated from Sophora alopecuroides L., and their concentrated filtrates were prepared as the fungal elicitors. The effects of different endophytic fungal elicitors at different induction time on the content of oxymatrine (OMA) and the activities of phenylalanine(PAL), peroxidase (POD), ascorbate peroxidase (APX) and catalase (CAT) in the tissue culture seedlings of S. alopecuroides L. were investigated. The results showed that culture broth concentrates from 4 species of endophytic fungi of S. alopecuroides L. obviously improved the content of OMA in tissue culture seedlings of S. alopecuroides L. Among them, culture broth concentrates of the endophytic fungi XKGKDF40 was most effective with 0~15 d of induction, OMA content in tissue culture seedlings of S. alopecuroides L. continuously increased, compared with the control, and reached the maximum of 3.616 mg·g-1 on the 15th day, which was 2.09 times that of the control group with the same induction time. Culture broth concentrates from 4 species of endophytic fungi of S. alopecuroides L. induced increase in activity of important PAL enzyme in alkaloid synthesis of tissue culture seedlings of S. alopecuroides L., and promoted the activities of antioxidant enzymes, such as POD, APX and CAT. The endophytic fungi XKGKDF40 was the most efficient elicitor on PAL activation, the enzyme activity reached a peak of 61.92 U·g-1 at 6th day after induction, which was 1.42 times that of the control group at the same induction time; Compared with other elicitors, the elicitor XKZKDF27 could induce POD activity significantly, after the induction in 12th day, POD activity reached the maximum of 62.30 U·mg-1, which is 2.70 times that of the control group at the same induction time; The elicitor XKZKDF11 also greatly improved the activities of APX and CAT in tissue culture seedlings of S. alopecuroides L., the enzyme activities reached the highest activity at the 12th day of induction (0.5538 U·mg-1 and 19.82 U·mg-1), which were 4.97 times and 3.00 times that of the control group at the same period respectively. The results indicated that the addition of endophytic fungal elicitors from S. alopecuroides L. not only activated the defensive response of the host tissue culture seedlings, but also promoted the synthesis of OMA of S. alopecuroides L..
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