史中飞,梁娟红,张小花,成宏斌,郑晟,王娟,张腾国.外源褪黑素对低温胁迫下油菜幼苗抗寒性的影响[J].干旱地区农业研究,2019,37(4):163~170
外源褪黑素对低温胁迫下油菜幼苗抗寒性的影响
Effect of exogenous melatonin on cold resistance of Brassica rapa seedlings under low temperature stress
  
DOI:10.7606/j.issn.1000-7601.2019.04.22
中文关键词:  白菜型油菜  褪黑素  低温胁迫  抗氧化酶  MAPKs基因表达
英文关键词:Brassica rapa  melatonin  low temperature stress  antioxidant enzyme  MAPKs gene expression
基金项目:国家自然科学基金(31460099)
作者单位
史中飞 School of Life Sciences, Northwest Normal University, Lanzhou, Gansu 730070,China 
梁娟红 School of Life Sciences, Northwest Normal University, Lanzhou, Gansu 730070,China 
张小花 School of Life Sciences, Northwest Normal University, Lanzhou, Gansu 730070,China 
成宏斌 School of Life Sciences, Northwest Normal University, Lanzhou, Gansu 730070,China 
郑晟 School of Life Sciences, Northwest Normal University, Lanzhou, Gansu 730070,China 
王娟 School of Life Sciences, Northwest Normal University, Lanzhou, Gansu 730070,China 
张腾国 School of Life Sciences, Northwest Normal University, Lanzhou, Gansu 730070,China 
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中文摘要:
      为了研究褪黑素(MT)对低温胁迫下油菜幼苗抗性的影响,以白菜型油菜“陇油6号”为试验材料,研究了外源MT对低温胁迫下黄瓜幼苗丙二醛(MDA)含量、相对电导率、叶绿素含量、活性氧(ROS)含量、抗氧化酶活性和MAPK3、MAPK4、MAPK6基因表达的影响。结果显示:与单独的4℃胁迫处理相比,100 μmol·L-1褪黑素预处理后再进行低温胁迫处理不同的时间(12、24、36、48 h),油菜幼苗MDA含量、相对电导率和ROS含量均显著降低,其中MDA和相对电导率分别降低了14.3%、15.0%、17.2%、20.0%和11.5%、12.8%、16.2%、15.1%,而叶绿素含量、三种抗氧化酶活性(CAT、POD、SOD)和MAPK3、MAPK4、MAPK6基因表达均增加,其中CAT的活性分别增加28.5%、23.1%、7.6%、13.1%,POD和SOD的活性分别增加36.0%、25.3%、5.9%、18.7%和14.6%、18.6%、9.1%、13.6%。表明低温胁迫下,100 μmol·L-1外源MT预处理可以显著降低油菜幼苗MDA含量、ROS水平和电解质的渗漏,增加叶绿素含量和抗氧化酶系统的活性,诱导MAPK3、MAPK4、MAPK6基因表达,降低质膜过氧化水平,保持细胞膜的完整性,增强油菜幼苗对低温的适应性。
英文摘要:
      The objective of this study was to investigate the effects of melatonin (MT) on the resistance of Brassica rape seedlings under low temperature stress. In this study, Brassica rapa “Longyou 6” was used as experimental material. The effects of exogenous MT on the relative conductivity, chlorophyll content, ROS content, antioxidant enzyme activity, and the expression of MAPK3, MAPK4, and MAPK6 genes of leaves in Brassica rape under low temperature stress were investigated. The results showed that compared with 4 ℃ stress treatment alone, under 100 μmol·L-1 MT pretreatment then treated with low temperature stress for different time (12, 24, 36, 48 h), the MDA content, relative conductivity and ROS content in Brassica rapa seedlings significantly decreased, MDA and relative conductivity were reduced by 14.3%, 15.0%, 17.2%, 20.0% and 11.5%, 12.8%, 16.2%, 15.1%, respectively, but chlorophyll content, three antioxidant enzyme activities (CAT, POD, SOD) and MAPK3, MAPK4, MAPK6 genes expression all increased by low temperature stress, the activity of CAT increased by 28.5%, 23.1%, 7.6%, and 13.1%, respectively, and the activities of POD and SOD increased by 36.0%, 25.3%, 5.9%, 18.7%, and 14.6%, 18.6%, 9.1%, 13.6%, respectively. So, we concluded that 100 μmol·L-1 exogenous MT pretreatment could significantly decreased the MDA content, reactive oxygen species (ROS) level, and electrolyte leakage, but increased chlorophyll content and activities of antioxidate system enzymes (CAT, POD and SOD), as induced MAPK3, MAPK4, and MAPK6 gene expression, decreased the membrane lipid peroxidation, maintained membrane integrity, and improved the adaptability of Brassica rape seedlings under low temperature stress.
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