| 张树武,徐秉良,刘佳,石成才.木霉耐盐突变菌株的紫外诱变选育[J].干旱地区农业研究,2019,37(4):263~268 |
| 木霉耐盐突变菌株的紫外诱变选育 |
| Identification of salinity tolerant mutant strain of Trichoderma sp. by ultraviolet mutagenesis |
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| DOI：10.7606/j.issn.1000-7601.2019.04.35 |
| 中文关键词: 木霉菌；紫外诱变；NaCl胁迫 生长；耐盐性 |
| 英文关键词:Trichoderma sp. ultraviolet mutagenesis NaCl stress growth salt tolerance |
| 基金项目:甘肃农业大学人才专项经费（2017RCZX-07）;甘肃农业大学科技创新基金（GAU-XKJS-2018-147）；甘肃农业大学国家重点实验室开放基金（GSCS-2017-1）;甘肃省农牧厅生物技术专项（GNSW-2013-19）;甘肃省国际科技合作专项（1604WKCA010） |
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| 中文摘要: |
| 为了获得高效耐盐木霉突变菌株，以盐碱土中分离的一株深绿木霉T-YM作为原始菌株进行紫外诱变处理，并筛选了突变菌株的最佳诱变条件，利用NaCl溶液模拟盐胁迫条件测定了突变菌株耐盐指标生长速率、产孢量和菌丝生长量。结果表明，与对照(野生型菌株)相比，当诱变时间为3 min时能够显著提高深绿木霉T-YM突变菌株菌落生长速度和产孢量，分别增加17.69%和28.82%；诱变时间为0.5 min和5 min时能够显著提高突变菌株菌丝生长量，分别增加42.22%和46.67%。利用10 mg·mL-1 NaCl溶液模拟盐胁迫条件时，与对照相比突变菌株菌落生长速度、产孢量和菌丝干重整体高于野生型菌株，尤其当诱变时间为3 min时，不同浓度盐胁迫下其产孢量平均增加51.18%，诱变时间为0.5 min时其菌丝干重平均增加23.65%；NaCl胁迫(10 mg·mL-1)下，诱变处理0.5 min获得的正突变菌株经传代接种培养后其耐盐性较为稳定。因此，紫外诱变可作为一种选育高效耐盐木霉突变菌株的有效方法。 |
| 英文摘要: |
| In order to obtain high efficient salt tolerant mutant strains of Trichoderma sp., we used the original strain of Trichoderma atroviride T-YM, which was isolated from the salinity soil to obtain the mutant strains by ultraviolet (UV) mutagenesis, and screened the ultraviolet mutagenesis parameters for the mutant strains, as well as determined the ability of salt tolerant parameters of colony diameters, dry weight and quantity of spore production for the mutant strains under different concentrations of NaCl solution. The results showed that the colony diameters and quantity of spore production by mutant strains were significantly higher after UV mutagenesis for 3 min. than that of original strain, and the colony diameters and quantity of spore production were increased by 17.69% and 28.82%, respectively, compared with the control (original strain). The dry weight of mycelia was significantly higher at 0.5 and 5 min, and was increased by 42.22% and 46.67%, respectively. Moreover, the colony diameters, dry weight, and quantity of spore production of mutant strains were higher than that of the original strain under NaCl stress. The average quantity of spore production was increased by 51.18% at 3 min, and the dry weight of mycelia was increased by 23.65% under different concentrations of NaCl solution. At the same time, the salt-tolerance of mutant strain was relatively stable after incubation for ten generations under NaCl stress (10 mg·mL-1) after UV mutagenesis for 0.5 min. Therefore, UV mutagenesis can be considered as an efficient method for screening and breeding the salt tolerant strains of Trichoderma sp.. |
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