赵方媛,田新会,杜文华.PEG-6000模拟干旱胁迫对15个小黑麦品系苗期生理特性的影响[J].干旱地区农业研究,2019,37(5):106~113
PEG-6000模拟干旱胁迫对15个小黑麦品系苗期生理特性的影响
Effects of PEG-6000 simulated drought stress on the physiological characteristics of 15 triticale varieties at the seedling stage
  
DOI:10.7606/j.issn.1000-7601.2019.05.16
中文关键词:  小黑麦  苗期  PEG-6000  生理特性  抗旱性评价
英文关键词:triticale  seedling stage  PEG-6000  physiological characteristics  drought resistance evaluation
基金项目:青海省青藏高原优良牧草品系资源利用重点实验室开放课题(2017-ZJ-Y12);西藏饲草产业专项(XZ201801NA02);甘肃省草地畜牧业可持续发展创新团队项目(2017C-11);国家重点研发计划(2018YFD0502402-3)
作者单位
赵方媛 College of Pratacultural Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Sino-U.S. Centers for Grazingland Ecosystem Sustainability, Gansu, Lanzhou 730070, China 
田新会 College of Pratacultural Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Sino-U.S. Centers for Grazingland Ecosystem Sustainability, Gansu, Lanzhou 730070, China 
杜文华 College of Pratacultural Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Sino-U.S. Centers for Grazingland Ecosystem Sustainability, Gansu, Lanzhou 730070, China 
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中文摘要:
      为研究15个小黑麦品系(Z1,Z3,Z5,Z6,Z9,Z12,Z19,Z21,Z23,Z36,Z41,Z48,Z49,Z50,Z55)苗期的抗旱性,本试验以甘农1号小黑麦(CK1)和新小黑麦5号(CK2)作为对照,设置正常灌水和干旱胁迫(30% PEG-6000)2个处理,于处理后28 d取样,测定叶片在正常灌水和干旱胁迫下叶绿素(CHL)、脯氨酸(Pro)、可溶性糖(SS)、丙二醛(MDA)含量及相对含水量(RWC),同时根据各指标与抗旱系数间的相关性,结合隶属函数,对参试小黑麦材料的抗旱性进行了综合评价。结果表明,小黑麦材料间各生理指标存在显著差异,Z9的平均RWC(77.26%)最高,Z49的平均CHL含量(0.55 mg·g-1)最高,Z1的平均Pro含量(0.36 mg·g-1)最高,CK2的平均SS含量(113.33 mg·g-1)最高,Z50的平均MDA含量(4.05 μmol·g-1)最高;干旱胁迫使小黑麦材料的平均RWC(正常灌水下为82.73%,干旱胁迫下为56.11%)显著降低,而干旱胁迫下的平均CHL(正常灌水下为0.33 mg·g-1,干旱胁迫下为0.40 mg·g-1)、Pro(正常灌水下为0.13 mg·g-1,干旱胁迫下为0.47 mg·g-1)、SS(正常灌水下为28.23 mg·g-1,干旱胁迫下为116.19 mg·g-1)及MDA(正常灌水下为2.50 μmol·g-1,干旱胁迫下为4.39 μmol·g-1)含量则显著升高;从小黑麦材料和干旱处理交互作用来看,干旱胁迫使所有参试小黑麦材料的RWC降低,其中Z12降幅最小,CK1降幅最大;CHL含量有升有降;Pro、SS及MDA含量均上升,增幅最小的分别为Z12、Z19和Z9,增幅最大的分别为CK1、Z3和Z50。根据抗旱性综合评价值(D值),可将供试小黑麦材料分为5种类型:Z1、Z5和CK1属于高抗旱型,Z3、Z6、Z36、Z41、Z48、Z49和Z50属于中抗旱型,Z21属于中间型,Z19、Z23、Z55和CK2属于干旱较敏感型,Z9和Z12属于干旱敏感型,因此说明甘农1号小黑麦和小黑麦品系Z1、Z5的抗旱性较强,适合在干旱半干旱雨养农业区种植。
英文摘要:
      In order to identify the drought resistance of 15 Triticosecale Wittmack lines (Z1,Z3,Z5,Z6,Z9,Z12,Z19,Z21,Z23,Z36,Z41,Z48,Z49,Z50,Z55) at the seedling stage, two triticale varieties, Gannong No.1 (CK1) and New triticale No.5 (CK2), were used as the controls. Two treatments including the normal irrigation and drought stress were set in this experiment that 30% of PEG-6000 was used to simulate the drought environment. Samples were collected at day 28 after treatment, and contents of chlorophyll (CHL), proline (Pro), soluble sugar (SS), malondialdehyde (MDA) and relative water content (RWC) in leaves of triticale materials were determined. In the meantime, according to the correlation between each index and drought-resistance coefficient, the drought resistance of triticale materials was evaluated comprehensively combined with the subordinate function. Results showed that significant differences existed in physiological parameters of triticale materials, which Z9 had the highest average RWC (77.26%), Z49 had the highest average CHL content (0.55 mg·g-1), Z1 had the highest average Pro content (0.36 mg·g-1), CK2 had the highest average SS content (113.33 mg·g-1), and Z50 had the highest average MDA content (4.05 μmol·g-1). The average RWC decreased significantly, while the average contents of CHL, Pro, SS and MDA increased significantly under the drought stress. The values of the physiological index under normal irrigation and drought stress were 82.73% and 56.11% (RWC), 0.33 mg·g-1 and 0.40 mg·g-1 (CHL), 0.13 mg·g-1 and 0.47 mg·g-1(Pro), 28.23 mg·g-1 and 116.19 mg·g-1(SS), 2.50 μmol·g-1 and 4.39 μmol·g-1 (MDA), respectively. The interaction of triticale materials and drought stress indicated that RWC decreased with that Z12 had the smallest decrease, CK1 had the largest decrease, CHL content changed inconsistently, and the contents of Pro, SS and MDA increased under the drought stress, Z12, Z19 and Z9 had the smallest increase, respectively, and CK1, Z3 and Z50 had the largest increase, respectively. Based on the comprehensive evaluation results, 17 triticale materials were divided into 5 groups according to the comprehensive evaluation value of drought resistance (D value), among which Z1, Z5, and CK1 belonged to the high drought resistance type, Z3, Z6, Z36, Z41, Z48, Z49 and Z50 belonged to the medium drought resistance type, Z21 belonged to the middle type, Z19, Z23, Z55 and CK2 belonged to the more sensitive drought type, Z9 and Z12 belonged to the sensitive drought type. Therefore, triticale variety Gannong No.1 and triticale lines Z1 and Z5 had strong drought resistance, which suitable for the semi-arid rain-fed areas.
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