白静,孙万仓,马骊,武军艳,蒲媛媛,何辉立,许德蓉,剡江梅,常瑜,徐春梅,方彦,李学才,牛早霞,盘荣德.北方白菜型冬油菜花器特性及农艺性状与含油量的相关性[J].干旱地区农业研究,2019,37(5):130~136
北方白菜型冬油菜花器特性及农艺性状与含油量的相关性
Correlation between floral characteristics, agronomic traits and oil content of winter rapeseed (Brassica rapa L.) in northern China
  
DOI：10.7606/j.issn.1000-7601.2019.05.19
中文关键词:  白菜型冬油菜  花器性状  农艺性状  含油量  相关性
英文关键词:Brassica rapa L.  floral traits  agronomic traits  oil content  correlation analysis
基金项目:国家现代农业产业技术体系(CARS-12)；国家自然科学基金(31460356,31560397)；973计划（2015CB150206）；科技部“油菜杂种优势利用技术与强优势杂交种创制”项目（2016YFD0101300）；国家农业科技成果转化项目（2014G10000317）；甘肃省自然科学基金(145RJZG050)
作者单位
白静 Gansu Provincial Engineering Research Center of Rapeseed, Gansu Provincial Key Laboratory of Arid Land Crop Sciences, Improvement and Key Laboratory of Crop Genetics and Planting Innovation of Gansu Province, College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
孙万仓 Gansu Provincial Engineering Research Center of Rapeseed, Gansu Provincial Key Laboratory of Arid Land Crop Sciences, Improvement and Key Laboratory of Crop Genetics and Planting Innovation of Gansu Province, College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
马骊 Gansu Provincial Engineering Research Center of Rapeseed, Gansu Provincial Key Laboratory of Arid Land Crop Sciences, Improvement and Key Laboratory of Crop Genetics and Planting Innovation of Gansu Province, College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
武军艳 Gansu Provincial Engineering Research Center of Rapeseed, Gansu Provincial Key Laboratory of Arid Land Crop Sciences, Improvement and Key Laboratory of Crop Genetics and Planting Innovation of Gansu Province, College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
蒲媛媛 Gansu Provincial Engineering Research Center of Rapeseed, Gansu Provincial Key Laboratory of Arid Land Crop Sciences, Improvement and Key Laboratory of Crop Genetics and Planting Innovation of Gansu Province, College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
何辉立 Gansu Provincial Engineering Research Center of Rapeseed, Gansu Provincial Key Laboratory of Arid Land Crop Sciences, Improvement and Key Laboratory of Crop Genetics and Planting Innovation of Gansu Province, College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
许德蓉 Gansu Provincial Engineering Research Center of Rapeseed, Gansu Provincial Key Laboratory of Arid Land Crop Sciences, Improvement and Key Laboratory of Crop Genetics and Planting Innovation of Gansu Province, College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
剡江梅 Gansu Provincial Engineering Research Center of Rapeseed, Gansu Provincial Key Laboratory of Arid Land Crop Sciences, Improvement and Key Laboratory of Crop Genetics and Planting Innovation of Gansu Province, College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
常瑜 Gansu Provincial Engineering Research Center of Rapeseed, Gansu Provincial Key Laboratory of Arid Land Crop Sciences, Improvement and Key Laboratory of Crop Genetics and Planting Innovation of Gansu Province, College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
徐春梅 Gansu Provincial Engineering Research Center of Rapeseed, Gansu Provincial Key Laboratory of Arid Land Crop Sciences, Improvement and Key Laboratory of Crop Genetics and Planting Innovation of Gansu Province, College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
方彦 Gansu Provincial Engineering Research Center of Rapeseed, Gansu Provincial Key Laboratory of Arid Land Crop Sciences, Improvement and Key Laboratory of Crop Genetics and Planting Innovation of Gansu Province, College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
李学才 Gansu Provincial Engineering Research Center of Rapeseed, Gansu Provincial Key Laboratory of Arid Land Crop Sciences, Improvement and Key Laboratory of Crop Genetics and Planting Innovation of Gansu Province, College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
牛早霞 Gansu Provincial Engineering Research Center of Rapeseed, Gansu Provincial Key Laboratory of Arid Land Crop Sciences, Improvement and Key Laboratory of Crop Genetics and Planting Innovation of Gansu Province, College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
盘荣德 Gansu Provincial Engineering Research Center of Rapeseed, Gansu Provincial Key Laboratory of Arid Land Crop Sciences, Improvement and Key Laboratory of Crop Genetics and Planting Innovation of Gansu Province, College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
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中文摘要:
      为研究北方白菜型冬油菜花冠直径与千粒重及含油量之间的相关性,以20份不同的白菜型冬油菜为材料，测定花器大小、农艺性状、种子含油量及蛋白质含量,并对控制花器特征基因mf6、apetala和myb进行实时荧光定量PCR分析。结果表明:花冠直径受雌蕊直径影响较大,主成分分析提取的3个主成分可综合为花器各性状长度、雌蕊直径和花器各性状宽度；以花冠直径为指示性状，将20份白菜型冬油菜花冠划分为3类群；相关性分析表明，花冠直径与千粒重呈极显著正相关，相关系数0.663；千粒重与含油量呈极显著正相关，与蛋白质含量呈显著负相关。相较于小花，mf6在大花中相对表达量上调了151.63%，而apetala和myb分别下调了83.80%和72.70%。北方白菜型冬油菜品种间花器大小存在显著差异；在育种中可通过增加花冠直径来提高千粒重，进而增加油菜产油量，培育高含油量白菜型冬油菜品种。mf6、apetala和myb基因对白菜型冬油菜花器生长发育均具有一定调控作用。
英文摘要:
      To study the correlation between the diameter of corolla and the 1000-grain weight and oil content of winter rapeseed (Brassica rapa L.) in northern China, twenty of different winter rapeseeds were used as materials to measure flower size, agronomic traits, seed oil content and protein content, and real-time quantitative PCR analysis was performed on the control gene mf6, apetala and myb. The results showed that the diameter of the corolla was greatly affected by the diameter of the pistil; The three principal components extracted from the principal component analysis could be integrated into the length of each trait of the flower, the diameter of the pistil, and the width of each trait of the flower; Using the diameter of the corolla as an indicator, 20 Chinese cabbage-type winter corollas were divided into three groups; Correlation analysis showed that the diameter of the corolla was significantly positively correlated with the 1000-grain weight, with a correlation coefficient of 0.663. 1000-grain weight was significantly positively correlated with oil content and significantly negatively correlated with protein content. Compared with florets, the relative expression of mf6 in large flowers were increased by 151.63%, while that of apetala and myb were down by 83.80% and 72.70%, respectively. There was a significant difference in flower sizes among winter rapeseed (Brassica rapa L.) varieties in northern China. In breeding, the 1000-grain weight can be increased by increasing the diameter of the corolla, thereby increasing the oil production of rapeseed and cultivating the high oil content winter rapeseed (Brassica rapa L.). mf6, apetala and myb genes can regulate the growth and development of floral of winter rape (Brassica rapa L.)
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