| 张凯煜,谷洁,王小娟,高华.生物有机肥对核桃园土壤细菌群落结构的影响[J].干旱地区农业研究,2019,37(6):178~183 |
| 生物有机肥对核桃园土壤细菌群落结构的影响 |
| Effects of bio\|organic fertilizer on soil bacterial community structure in walnut orchard |
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| DOI：10.7606/j.issn.1000-7601.2019.06.26 |
| 中文关键词: 生物有机肥 细菌群落 高通量测序 核桃园土壤 |
| 英文关键词:Bio\|organic fertilizer bacterial community high\|throughput sequencing walnut orchard soil |
| 基金项目:国家林业局林业科学技术项目（\[2016\]43）; 国家自然科学基金（41671474） |
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| 中文摘要: |
| 为了探究生物有机肥对核桃园土壤细菌群落的影响，采用16s rRNA高通量测序技术，研究了不施肥（CK）、常规施肥（TN）和施生物有机肥（TB）3个处理土壤细菌群落多样性和结构组成。结果表明，TB处理中土壤有机质、全氮、全磷比CK处理分别提高了46.3%、30.6%和50.8%。TB处理α-多样性指数均为最高，而TN处理中香浓指数和ACE指数较CK处理分别降低了0.5% 和1.2%。在门水平上，变形菌门（Proteobacteria）、放线菌门（Actinobacteria）、酸杆菌门（Acidobacteria）、拟杆菌门（Bacteroidetes）、芽单胞菌门（Gemmatimonadetes）为优势类群，共占细菌总量的85.2%~90.6%。鞘脂单细胞菌属（Sphingomonas）和节杆菌属（Arthrobacter）为主要属，其相对丰度在TB处理较CK处理分别增加了17.6%和56.6%，较TN处理分别增加了51.5%和104.3%。冗余分析结果表明，环境因子解释了细菌群落变化的94.6%，土壤有机质、碱解氮和速效钾含量是造成核桃园土壤细菌群落结构差异的主要原因。 |
| 英文摘要: |
| A field experiment was conducted to investigate the effect of bio\|organic fertilizer on the bacterial community diversity in walnut orchard. High\|throughput sequencing was used to study the bacteria diversity and composition in different fertilization treatments. An analysis of the relationship between bacterial community and environmental factors was conducted as well. There were three treatments including control (CK), conventional fertilizer treatment (TN) and bio\|organic fertilizer (TB). The results indiated that soil organic matter, total nitrogenandtotal phosphorus of TB were 46.3%, 30.6% and 50.8% higher than those of CK, respectively. The α-diversity index of TB was higher than that of TN and CK. While the ACE index and Shannon index of TN were 0.5% and 1.2% lower than that of CK treatment, respectively. At the phylum level, Proteobacteria, Acidobacteria, Actinobacteria, Bacteroidetes and Gemmatimonadete were the predominant phyla, accounting for 85.2%~90.6% of the total reads. At the genera level, Sphingomonas and Arthrobacter were predominant genus. The relative abundance of Sphingomonas and Arthrobacter in TB were increased by 17.6% and 56.6% than that in CK, and 51.5% and 104.3% than that in TN, respectively.Redundancy analysis showed that environmental factors explained 94.6% of bacterial community changes. Soil organic matter,alkali\|hydrolyzable nitrogen and potassium contents were the main factors for the bacterial community changes in walnut garden. |
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