岳思君,冯翠娥,杨彦研,陈丽萍,郭洋,郑蕊,苏建宇.不同连作年限硒砂瓜土壤细菌群落结构特征[J].干旱地区农业研究,2020,38(1):230~236
不同连作年限硒砂瓜土壤细菌群落结构特征
Analysis of microbial community structure and diversity in selenium\|sand melon soil under different continuous cropping years
  
DOI:10.7606/j.issn.1000-7601.2020.01.30
中文关键词:  连作年限;硒砂瓜  细菌群落结构;多样性;高通量测序技术
英文关键词:high throughput sequencing technique  continuous cropping  bacterial community structure  diversity
基金项目:国家自然科学基金(31360025,31560418);宁夏回族自治区重点研发计划项目(东西部合作专项:2017BY081)
作者单位
岳思君 Western Biological Resources Protection and Utilization Lab of National Education Ministry, College of Life Sciences, Ningxia University, Yinchuan, Ningxia, 750021, China 
冯翠娥 Western Biological Resources Protection and Utilization Lab of National Education Ministry, College of Life Sciences, Ningxia University, Yinchuan, Ningxia, 750021, China 
杨彦研 Western Biological Resources Protection and Utilization Lab of National Education Ministry, College of Life Sciences, Ningxia University, Yinchuan, Ningxia, 750021, China 
陈丽萍 Western Biological Resources Protection and Utilization Lab of National Education Ministry, College of Life Sciences, Ningxia University, Yinchuan, Ningxia, 750021, China 
郭洋 Western Biological Resources Protection and Utilization Lab of National Education Ministry, College of Life Sciences, Ningxia University, Yinchuan, Ningxia, 750021, China 
郑蕊 Western Biological Resources Protection and Utilization Lab of National Education Ministry, College of Life Sciences, Ningxia University, Yinchuan, Ningxia, 750021, China 
苏建宇 Western Biological Resources Protection and Utilization Lab of National Education Ministry, College of Life Sciences, Ningxia University, Yinchuan, Ningxia, 750021, China 
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中文摘要:
      应用高通量测序技术对宁夏中卫地区不同连作年限硒砂瓜土壤细菌群落结构和多样性进行研究,旨在揭示不同连作年限对硒砂瓜土壤细菌群落结构的影响。结果表明:随着连作时间的增加,细菌多样性指数和丰富度指数逐渐增加。从5份不同连作年限硒砂瓜土壤样本中共获得39门、98纲和620属的细菌;优势门为放线菌门(Actinobacteria)、变形菌门(Proteobacteria)、绿弯菌门(Chloroflexi)、酸杆菌门(Acidobacteria);随着连作时间的增加,放线菌门、酸酐菌门丰度先增加后降低,变形菌门丰度逐渐降低,绿弯菌门丰度呈先降低后增加又降低现象;优势纲分别为放线菌纲(Actinobacteria)、酸杆菌纲(Acidobacteria)、α-变形菌纲(Alphaproteobacteria)、芽单胞菌纲(Gmmatimonadetes)、杆菌纲(Bacilli)、热原体纲(Themomicrobia)、γ-变形菌纲(Gammaproteobacteria)、β-变形菌纲(Betaproteobacteria);优势目为norank_c_Acidobacteria、芽孢杆菌目(Bacillales)、芽单胞菌目(Gemmatimonadales)、根瘤菌目(Rhizobiales)。RDA分析表明,有机质、速效磷是硒砂瓜连作土壤细菌群落组成的主要影响因子。研究表明,引起硒砂瓜连作障碍发生主要因素不是由于土壤理化性质变化引起的,而是随着连作时间的增加,放线菌门、变形菌门、绿弯菌门、酸杆菌门、厚壁菌门等有益微生物丰度下降造成的。
英文摘要:
      High\|throughput sequencing technique was used to study the soil bacterial community structure and diversity of selenium\|sand melon soil in Zhongwei area of Ningxia under different continuous cropping years. The results showed that bacterial diversity index and richness index gradually increased with the increase in continuous cropping years. A total of 39 phyla, 98 classes, and 620 genera of bacteria were isolated from 5 samples of selenium\|sand melon soil with different successive cropping years. The dominant phylum was Actinobacteria, Proteobacteria, Chloroflexi and Acidobacteria. With the continuous cropping time increased, the abundance of Actinobacteria and Acidobacteria increased first and then decreased, the abundance of Proteobacteria gradually decreases, the abundance of Chloroflexi decreased first, then increased and then decreased. The dominant class was Actinobacteria, Acidobacteria, Alphaproteobacteria, Gmmatimonadetes, Bacilli, Thermomicrobia, Gammaproteobacteria and Betaproteobacteria. The dominant order was norank_c_Acidobacteria, Bacillales, Gemmatimonadales, Rhizobiales. The dominant orders were norank_c_Acidobacteria, Bacillales, Gemmatimonadales, and Rhizobiales. RDA analysis showed that organic matter and available phosphorus were the main influencing factors of bacterial community composition in selenium\|sand melon continuous cropping soil. The results showed that the occurrence of continuous cropping obstacle of selenium\|sand melon was not caused by the change of soil physical and chemical properties, but was caused by the decrease of the abundance of beneficial microorganisms such as Actinobacteria. Proteobacteria. Chloroflexi, Acidobacteria and Firmicutes with the increase of continuous cropping time.
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