| 秦彬,张明聪,何松榆,张春宇,王明瑶,金喜军,王孟雪,张玉先,胡国华.褪黑素浸种对大豆种子萌发过程中干旱胁迫的缓解效应[J].干旱地区农业研究,2020,38(2):192~198 |
| 褪黑素浸种对大豆种子萌发过程中干旱胁迫的缓解效应 |
| Alleviating effect of melatonin soaking on drought stress during soybean seed germination |
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| DOI：10.7606/j.issn.1000-7601.2020.02.27 |
| 中文关键词: 大豆；干旱胁迫 褪黑素浸种；萌发；根系生长 生理机制 |
| 英文关键词:soybean drought melatonin seed soaking germination root growth physiological mechanism |
| 基金项目:国家自然科学基金项目（31801303）;黑龙江省农垦总局重点科研计划项目（HKKY190206\|01）；黑龙江八一农垦大学博士科研启动基金（XYB2014-04）；国家现代农业产业技术体系（CARS-04-01A） |
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| 中文摘要: |
| 以大豆干旱敏感型品种绥农26为材料，采用室内培养法，研究不同浓度褪黑素( 0、100、300、500、800 μmol·L-1) 浸种对6%PEG-6000 模拟干旱胁迫条件下大豆种子萌发的影响。结果表明:干旱胁迫处理抑制了大豆种子萌发，与对照（蒸馏水处理种子）相比,处理D（6% PEG处理种子）的发芽势、发芽率、根体积和鲜重显著降低，降幅分别为38.14%、23.72%、21.4%、25.6%；根系电导率、MDA和H2O2含量大幅上升，增幅分别为51.18%、83.33%和60.60%。而褪黑素浸种处理（100、300、500、800 μmol·L-1）显著缓解了干旱胁迫对种子萌发的抑制，促进了根系生长，其中以根直径和干重提升最为显著，分别增加了16.38%、29.73%、34.97%、31.72%和10.70%、14.28%、10.70%、25.00%；抗氧化酶(SOD、POD、CAT、APX)活性提高，其中处理M500+D（500 μmol·L-1褪黑素+6%PEG）效果最为显著，增幅分别为37.61％、40.10％、12.96%和28.31%。在6% PEG-6000模拟的干旱胁迫下，500 μmol·L-1褪黑素浸种处理对大豆种子萌发过程中干旱胁迫的缓解效果最佳。 |
| 英文摘要: |
| The drought\|sensitive soybean variety, Suinong 26, was used as the material to study the effects of different concentrations of melatonin (0, 100, 300, 500 μmol·L-1 and 800 μmol·L-1) soaking soybean seeds on the germination under simulated drought stress, 6% PEG-6000. The results showed that the germination of soybean seeds was inhibited through the drought stress treatment. Compared with the control, the germination energy, germination ratio, root volume, dry weight and fresh weight in the groups treated by D (6% PEG) significantly decreased by 38.14%, 23.72%, 21.4%, and 25.6%, respectively. The root conductivity, MDA and H2O2 content significantly increased by 51.18%, 83.33%, and 60.60%, respectively. Besides, the seed germination and the growth of roots under the drought stress were significantly promoted with the melatonin soaking treatment. The root diameter and dry weight significantly increased 16.38%, 29.73%, 34.97%, 31.72% and 10.70%, 14.28%, 10.70%, 25.00%, respectively. The activities of antioxidant enzymes (SOD, POD, CAT and APX) were further improved, among which they were increased most significantly with the treatment of M 500 + D (500 μmol·L-1 melatonin + 6% PEG) 37.61%, 40.10%, 12.96%, and 28.31%, respectively. It is preliminarily deduced that under 6% PEG-6000 simulated drought stress, the melatonin soaking with melatonin of 500 μmol·L-1 was the best treatment. |
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