| 赵小强,方鹏,彭云玲,张金文.低温胁迫下不同玉米自交系对外源甜菜碱的生理响应[J].干旱地区农业研究,2020,38(3):81~88 |
| 低温胁迫下不同玉米自交系对外源甜菜碱的生理响应 |
| Physiological response of different maize inbred lines to exogenous GB under low\|temperature stress |
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| DOI：10.7606/j.issn.1000-7601.2020.03.11 |
| 中文关键词: 玉米自交系 低温胁迫 外源甜菜碱 萌发期 苗期 生理响应 |
| 英文关键词:maize inbred line low\|temperature stress exogenous GB germination stage seedling stage physiological response |
| 基金项目:甘肃农业大学科技创新基金-公招博士科研启动基金（GAU-KYQD-2019-19）；甘肃农业大学甘肃省干旱生境作物学重点实验室开放基金（GSCS-2019-8）；甘肃省高等学校创新能力提升项目（2019A-052）；兰州青绿仪器技术有限公司横向项目（WT20191025） |
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| 中文摘要: |
| 为了探究外源甜菜碱（GB）提高玉米萌发期和苗期耐寒性的响应机制及比较外源GB对不同玉米时期低温伤害的缓解效果，采用不同浓度外源GB处理耐寒性不同的6份玉米自交系，研究10℃低温胁迫下外源GB对玉米种子萌发及幼苗生理特性的影响。结果表明，低温胁迫能明显抑制玉米种子萌发，其平均发芽势、发芽率、胚芽长、胚根长、胚芽鲜重和胚根鲜重分别降低了55.2%、45.4%、64.6%、61.3%、57.7%和71.0%；幼苗叶片明显受到伤害，其平均相对电导率、丙二醛含量、脯氨酸含量、可溶性糖含量、SOD活性、POD活性和CAT活性分别升高了282.7%、150.1%、140.6%、124.7%、374.4%、209.7%和211.3%。萌发期和苗期分别添加20 μmol·L-1和10 μmol·L-1外源GB对低温胁迫下玉米的缓解效果最佳，其能明显促进玉米种子萌发及减轻幼苗生理伤害，除相对电导率（42.4%）和丙二醛含量（30.5%）显著降低外，脯氨酸、可溶性糖含量及抗氧化酶活性均显著升高（12.6%~324.9%）。在此最佳低温缓解外源GB浓度下，以萌发和幼苗生理性状的低温缓解指数作为低温缓解评价指标，采用隶属函数法综合评价两时期不同自交系的低温缓解效果，发现萌发期外源GB对玉米的缓解效果（0.585）优于苗期（0.454）。 |
| 英文摘要: |
| To explore the response mechanism of exogenous glycine betaine (GB) on improving the cold resistance in maize and to compare the cold mitigation effect of exogenous GB at germination and seedling stages, six different maize inbred lines were conducted under different concentrations of exogenous GB, and the effects of exogenous GB on seed germination and seedling physiological trait across 10℃ low temperature stress were analysed. It showed that maize seeds germinations were significantly inhibited and the seedlings were clearly hurt under low temperature stress, thus, the germination potential, germination rate, plumula length, radical length, shoot fresh weight and root fresh weight were decreased 55.2%, 45.4%, 64.6%, 61.3%, 57.7% ，and 71.0%, respectively, and relative conductivity, malondiadehyde content, proline content, soluble sugar content, SOD activity, POD activity and CAT activity were increased 282.7%, 150.1%, 140.6%, 124.7%, 374.4%, 209.7%, and 211.3%, respectively. 20 μmol·L-1 and 10 μmol·L-1 GB had the largest cold mitigation effect at germination and seedling stages, respectively, which increased seed germination and decreased seedling physiological injury, excluding relative conductivity (42.4%) and malondiadehyde content (30.5%) were significant decreased, the content of proline，soluble suger and the activity of antioxidant enzyme were significantly increased(12.6%~324.9%). Under the optimal exogenous GB concentration, the GB cold ease index (EI) of corresponding traits as evaluation indexes, then using the membership function method to comprehensively evaluate cold mitigation effect of different inbred lines at two stages, and the cold mitigation effect of exogenous GB at germination stage (0.585) that were better than seedling stage (0.454). |
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