| 曹雪松,郑和祥,佟长福,李和平,刘瑞春,郝语新.微纳米气泡水地下滴灌对紫花苜蓿土壤酶活性与根系脯氨酸的影响[J].干旱地区农业研究,2020,38(4):67~73 |
| 微纳米气泡水地下滴灌对紫花苜蓿土壤酶活性与根系脯氨酸的影响 |
| Effects of subsurface drip irrigation with micro\|nano bubbled water on soil enzyme activity and root prolineof alfalfa |
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| DOI：10.7606/j.issn.1000-7601.2020.04.09 |
| 中文关键词: 地下滴灌 微纳米气泡水 紫花苜蓿 土壤酶活性 根系特征 根系脯氨酸含量 |
| 英文关键词:subsurface drip irrigation micro\|nano bubbled water alfalfa soil enzyme activity root characteristics root proline content |
| 基金项目:中国水利水电科学研究院科研专项（MK2018J05）；国家青年科学基金（41901052）；国家“十三五”重点研发计划项目（2017YFC040320504）；水利部灌溉试验重点站（内蒙古鄂尔多斯灌溉试验站-2020年任务） |
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| 中文摘要: |
| 为了探究微纳米气泡水地下滴灌条件下不同溶解氧质量浓度对紫花苜蓿土壤酶活性与根系脯氨酸的影响，采用田间试验方法，在地下滴灌灌溉定额相同的条件下设低（WA-1,1.8 mg·L-1）、中（WA-2,5.0 mg·L-1）、高（WA-3,8.2 mg·L-1）3个微纳米气泡水溶氧量质量浓度水平和不加气常规灌溉处理（CK），研究了不同试验处理下紫花苜蓿根际土壤过氧化氢酶、脲酶活性以及根系特征和游离脯氨酸含量的变化。结果表明：微纳米气泡水地下滴灌能明显增加紫花苜蓿根区土壤过氧化氢酶和脲酶活性；在紫花苜蓿不同的生长期，随着微纳米气泡水溶解氧质量浓度的增加，土壤过氧化氢酶和脲酶活性呈增大的趋势；相同试验处理条件下，土壤过氧化氢酶和脲酶活性随着微纳米气泡水地下滴灌次数的增加呈先升后降的趋势；处理WA-3、WA-2、WA-1的土壤过氧化氢酶活性和脲酶活性最大分别为20.28、19.19、16.92 ml·g-1和13.98、13.17、12.07 mg·g-1·d-1，分别比处理CK（13.82 ml·g-1和9.63 mg·g-1·d-1）增加46.74%、38.86%、22.43%和45.17%、36.76%和25.34%；微纳米气泡水地下滴灌能降低根系游离脯氨酸含量，处理WA-2根系游离脯氨酸含量最低（51.01μmol·g-1），相较于处理CK(92 μmol·g-1)，减少44.55%。说明微纳米气泡水地下滴灌能够缓解因长时间地下滴灌导致的土壤通透性减弱的现象，提高土壤氧气扩散率，增强土壤酶活性，促进根系的正常生长发育。 |
| 英文摘要: |
| In order to explore the effects of different dissolved oxygen levels on soil enzyme activity and root proline of alfalfa under the condition of subsurface drip irrigation with micro\|nano bubbled water, field trials were used with the same irrigation quota. Three dissolved oxygen mass concentration levels of micro\|nano bubbled water (low: 1.8 mg·L-1, WA-1; medium: 5.0 mg·L-1, WA-2; and high: 8.2 mg·L-1, WA-3) and unfilled conventional irrigation treatment (CK) were set to study the status of catalase, urease activity, root characteristics, and free proline content in roots of alfalfa rhizosphere under different treatments. The results showed that subsurface drip irrigation with micro\|nano bubbled water significantly increased the activity of soil catalase and urease in the root zone of alfalfa.During the period, with increasing the dissolved oxygen mass concentration of micro\|nano bubbled water, the activities of soil catalase and urease showed an increasing trend. Under the same experimental treatment conditions, the activities of soil catalase and urease showed a trend of rising first and then decreasing with increasing the number of subsurface drip irrigation with micro\|nano bubbled water. The maximum soil catalase and urease activities of WA-3, WA-2, and WA-1 were 20.28, 19.19, 16.92 ml·g-1 and 13.98, 13.17, 12.07 mg·g-1·d-1, respectively, equivalent to increases of 46.74%, 38.86%, 22.43% and 45.17%, 36.76%, 25.34%, respectively, compared with 13.82 ml·g-1 and 9.63 mg·g-1·d-1for CK. Micro\|nano bubbled water subsurface drip irrigation reduced the free proline content of roots and treatment with WA-2 had the lowest free proline content at 51.01 μmol·g-1, which was a decrease by 44.55% compared with 92 μmol·g-1 for CK. This shows that subsurface drip irrigation with micro\|nano bubbled water alleviated the phenomenon of weakened soil permeability caused by long\|term subsurface drip irrigation, improved soil oxygen diffusion rate, enhanced soil enzyme activity, promoted aerobic respiration of root system and the growth and development of the whole plant. |
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