| 刘建新,刘瑞瑞,贾海燕,卜婷,李娜.外源H2S对渗透胁迫下裸燕麦种子萌发和幼苗生长的影响[J].干旱地区农业研究,2020,38(6):61~67 |
| 外源H2S对渗透胁迫下裸燕麦种子萌发和幼苗生长的影响 |
| Effect of exogenous hydrogen sulfide on seed germination and seedling growth in naked oat under osmotic stress |
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| DOI：10.7606/j.issn.1000-7601.2020.06.09 |
| 中文关键词: 硫化氢 裸燕麦 渗透胁迫 种子萌发 幼苗生长 |
| 英文关键词:hydrogen sulfide(H2S) naked oat osmotic stress seed germination seedling growth |
| 基金项目:国家自然科学基金(31960375)；甘肃省自然科学基金（20JR5RA491） |
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| 中文摘要: |
| 为探讨新型气体信号硫化氢(H22S)对渗透胁迫下作物种子萌发和幼苗生长的影响，以裸燕麦(Avena nude)品种‘定莜9号’种子为材料，采用H22S供体硫氢化钠(NaHS)浸种浓度和聚乙二醇(PEG-6000)模拟渗透胁迫两因素试验，其中NaHS浓度设0、0. 2、1. 0 mmol·L-1和5. 0 mmol·L-14个水平，PEG浓度设0、119、149、178 g·L-1和202 g·L-15个水平，进行完全组合设计，共20个处理，测定不同处理裸燕麦种子萌发参数(发芽势、发芽率、发芽指数、活力指数、平均发芽时间和贮藏物质转运率)和幼苗生长参数(胚根长、胚芽长和幼苗干重)，运用隶属函数分析方法对不同处理种子的耐渗透能力进行综合评价。结果表明：（1）NaHS浓度和PEG浓度对各萌发参数和幼苗生长参数均有显著影响；两者的交互作用对发芽指数、活力指数、平均发芽时间和胚根长影响显著。（2）在同一NaHS浓度下，随着PEG浓度提高，各萌发参数和幼苗生长参数均显著下降，而平均发芽时间呈增加趋势。（3）在相同PEG浓度下，0.2 mmol·L-1NaHS浸种对各萌发参数和幼苗生长参数均无显著影响；1.0 mmol·L-1NaHS浸种提高了发芽指数、活力指数、贮藏物质转运率、胚根长、胚芽长和幼苗干重，降低了平均发芽时间；5.0 mmol·L-1NaHS浸种提高了平均发芽时间，而其余各萌发参数和幼苗生长参数均呈下降趋势。（4）利用隶属函数综合分析得知，用1.0 mmol·L-1NaHS浸种可提高PEG渗透胁迫下的综合评价D值，而5.0 mmol·L-1NaHS浸种处理的D值显著下降。由此表明，适宜浓度NaHS (1.0 mmol·L-1)浸种能够促进渗透胁迫下裸燕麦的种子萌发和幼苗生长。 |
| 英文摘要: |
| Hydrogen sulfide (H2S) is a novel type gas signaling molecule in plants. The effects of exogenous H2S on the seed germination and seedling growth of naked oat (Avena nude) under osmotic stress were investigated．Seeds of naked oat cultivar ‘Dingyou 9’ were used as materials for a two\|factor experiment including concentration of sodium hydrosulfide (NaHS, a donor of H2S) and osmotic stress. Four different concentrations of NaHS (0, 0.2, 1.0, and 5.0 mmol·L-1) were set to soak the seeds for 8 hours, and osmotic stress was simulated using five different concentrations of polyethylene glycol (PEG-6000) (0, 119, 149, 178 g·L-1, and 202 g·L-1). A total of 20 treatments were conducted to determine germination characteristics (germination potential, germination rate, germination index, vigor index, mean germination time, and storage material transformation rate) and seedlings growth parameters (radicle length, plumule length, and seedling dry weight). Subordinate function analysis was also carried out for comprehensive evaluation of osmotic tolerance of naked oat seeds in different treatments. The results showed that: (1) The NaHS concentration and osmotic stress had significant effects on the parameters of seed germination and seedling growth of naked oat. The interaction between them had significant effects on germination index, vigor index, mean germination time, and radicle length. (2) With the enhancement of osmotic stress, which was simulated by PEG, the all parameters of seed germination and seedling growth decreased significantly under the same NaHS concentration, but only the average germination time increased dramatically. (3) At the same PEG concentration, soaking seeds with 0.2 mmol·L-1 NaHS had no significant effect on the parameters of seed germination and seedling growth of naked oats. Soaking seeds with 1.0 mmol·L-1 NaHS increased germination index, vigor index, storage material transformation rate, radicle length, plumule length, and seedling dry weight, while decreased average germination time. Soaking seeds with 5.0 mmol·L-1 NaHS increased the mean germination time, while all other seed germination and seedling growth parameters showed a decreasing trend. (4) The result of subordinate function analysis showed that the soaking seeds with 1.0 mmol·L-1 NaHS improved the comprehensive evaluation value (D) under osmotic stress simulated by PEG, while the D value of soaking seeds with 5.0 mmol·L-1 NaHS significantly decreased. These results indicated that soaking seeds with appropriate concentration of NaHS (1.0 mmol·L-1) can effectively improve the seed vigor of naked oats and promote its seed germination and seedling growth under osmotic stress. |
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