外源5-氨基乙酰丙酸对干旱胁迫下玉米幼苗生理特性及抗氧化酶基因表达的影响

王鹏; 王铁兵; 王瑞; 闫蕊; 史珍; 常巧玲; 王芳

王鹏,王铁兵,王瑞,闫蕊,史珍,常巧玲,王芳.外源5-氨基乙酰丙酸对干旱胁迫下玉米幼苗生理特性及抗氧化酶基因表达的影响[J].干旱地区农业研究,2021,39(1):75~81

外源5-氨基乙酰丙酸对干旱胁迫下玉米幼苗生理特性及抗氧化酶基因表达的影响

Effects of exogenous 5-ALA on physiological characteristics and antioxidant enzyme gene expression of maize seedlings under drought stress

DOI：10.7606/j.issn.1000-7601.2021.01.10

中文关键词: 玉米 5-氨基乙酰丙酸干旱胁迫生理特性抗氧化酶基因

英文关键词:maize 5-ALA drought stress physiological characteristics antioxidant enzyme gene

基金项目:甘肃农业大学青年导师基金项目（GAU-QDFC-2019-01）资助

作者	单位
王鹏	甘肃农业大学农学院/甘肃省干旱生境作物学重点实验室/甘肃省作物遗传改良与种质创新重点实验室，甘肃兰州 730070
王铁兵	甘肃农业大学农学院/甘肃省干旱生境作物学重点实验室/甘肃省作物遗传改良与种质创新重点实验室，甘肃兰州 730070
王瑞	酒泉市种子管理站，甘肃酒泉 735000
闫蕊	酒泉市种子管理站，甘肃酒泉 735000
史珍	甘肃农业大学农学院/甘肃省干旱生境作物学重点实验室/甘肃省作物遗传改良与种质创新重点实验室，甘肃兰州 730070
常巧玲	甘肃农业大学农学院/甘肃省干旱生境作物学重点实验室/甘肃省作物遗传改良与种质创新重点实验室，甘肃兰州 730070
王芳	甘肃农业大学农学院/甘肃省干旱生境作物学重点实验室/甘肃省作物遗传改良与种质创新重点实验室，甘肃兰州 730070

摘要点击次数: 1275

全文下载次数: 205

中文摘要:

以玉米品种郑单958为试验材料，研究外源5-氨基乙酰丙酸（5-ALA）对15% PEG-6000模拟干旱胁迫下玉米幼苗生理特性及抗氧化酶基因表达的影响。结果表明：与对照相比，干旱胁迫下玉米幼苗叶片中超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性，丙二醛(MDA)、脯氨酸(Pro)和可溶性糖含量显著增加，分别增加132.69%、30.69%、30.39%、60.34%、495.65%、31.36%，叶绿素含量降低21.33%（P＜0.05），净光合速率(Pn)、气孔导度(Gs)、胞间CO₂浓度(Ci)、蒸腾速率(Tr)、最大光化学效率(F_v/F_m)、实际光化学效率(ФPSⅡ)和光化学猝灭系数(qP)都显著下降，分别下降30.79%、68.75%、9.15%、53.15%、33.33%、47.06%、26.37%，但非光化学猝灭系数(NPQ)上升31.82%（P＜0.05）。喷施25 mg·L^-1的5-ALA能显著缓解干旱胁迫对玉米幼苗造成损伤，与干旱胁迫下相比，抗氧化物酶SOD、POD、CAT活性分别增加17.63%、17.68%、27.48%（P＜0.05），Pro、可溶性糖和叶绿素含量分别增加34.97%、19.64%和17.82%（P＜0.05），MDA含量降低22.12%（P＜0.05），Pn、Gs、Ci、Tr、F_v/F_m、ФPSⅡ、qP分别上升79.03%、180.00%、35.77%、161.54%、20.00%、88.89%、26.87%（P＜0.05），NPQ下降15.52%（P＜0.05）。实时荧光定量PCR得到干旱胁迫下喷施25 mg·L^-1的5-ALA，SOD3、POD3、CAT1三种抗氧化酶基因表达量也显著增加，较干旱胁迫下不喷施5-ALA分别增加了49.31%、34.07%、44.17%。说明外源5-ALA对干旱胁迫下玉米幼苗的生长具有缓解作用，能增强玉米幼苗的抗旱性。

英文摘要:

Taking maize variety Zhengdan 958 as experimental material, the effects of exogenous 5-ALA on physiological characteristics and antioxidant enzyme gene expression of maize seedlings under simulated drought stress with 15% PEG-6000 were studied.The results showed that the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), the contents of malondialdehyde (MDA), proline (Pro), and soluble sugarincreased by 132.69%, 30.69%, 30.39%, 60.34%, 497.65%, and 31.36%, respectively; the contents of chlorophyll decreased by 21.33%; the net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO₂ concentration (Ci), transpiration rate (Tr), the maximum photochemical efficiency (F_v/F_m), the actual photochemical efficiency (ФPSⅡ), and the photochemical quenching coefficient (qP) decreased significantly by 30.79%, 68.75%, 9.15%, 53.15%, 33.33%, 47.06%, and 26.37%, respectively, but the non\|photochemical quenching coefficient (NPQ) increased significantly by 31.82% in the leaves of maize seedlings under drought stress. Spraying 25 mg·L^-1 of 5-ALA obviously relieved that damage to maize seedlings caused by drought stress, compared with PEG treatment, the activities of SOD, POD, and CAT increased by 17.63%, 17.68%, and 27.48%, respectively. The contents of Pro, soluble sugar, and chlorophyll increased by 34.97%, 19.64%, and 17.82%, respectively, the contents of MDA decreased by 22.12%, and the photosynthetic fluorescence parameters Pn, Gs, Ci, Tr, F_v/F_m, ФPSⅡ, and qP increased by 79.03%, 180%, 35.77%, 161.54%, 20.00%, 88.89%, and 26.87%, respectively, and the NPQ decreased by 15.52%. At the same time, the expressions of SOD3, POD3, and CAT1 antioxidant enzyme genes obtained by real\|time fluorescence quantitative PCR also increased by 49.31%, 34.07%, and 44.17%, respectively. In conclusion, 5-ALA can alleviate the growth of maize seedlings under drought stress and enhance the drought resistance of maize seedlings.

查看全文查看/发表评论下载PDF阅读器