| 方淑梅,侯雪,邱草威,韩蓓,胡俊杰,梁喜龙.大豆DNA脱甲基化酶相关基因的克隆及连作胁迫应答[J].干旱地区农业研究,2021,39(1):82~86 |
| 大豆DNA脱甲基化酶相关基因的克隆及连作胁迫应答 |
| Cloning of soybean DNA demethylase related genes and response to continuous cropping stress |
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| DOI：10.7606/j.issn.1000-7601.2021.01.11 |
| 中文关键词: DNA脱甲基化酶 基因克隆 基因表达 连作综合逆境 连作胁迫应答 大豆 |
| 英文关键词:DNA demethylase gene cloning gene expression continuous cropping comprehensive stress response to continuous cropping stress soybean |
| 基金项目:黑龙江八一农垦大学人才科研启动计划（XDB-2016-23）；大学生创新创业训练计划项目（201910223012，XC2018057）；国家自然科学基金（31201163）;国家重点研发计划（298YFD0201004-6） |
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| 中文摘要: |
| 利用大豆基因组数据库Phytozome v12.1.6获得大豆DNA脱甲基化相关基因Glyma03g34860和Glyma10g07601的CDS序列，以大豆叶片RNA为模板，RT-PCR克隆获得Glyma03g34860基因大小为5 226 bp，Glyma10g07601基因大小为6 045 bp。利用STRING在线软件预测这两个转录本的互作蛋白质完全一致，主要互作蛋白8个，包括一个AP位点裂解酶和2个RNA聚合酶亚基;采用qRT-PCR分析他们对大豆连作综合逆境胁迫的响应。结果表明：连作综合逆境胁迫使Glyma03g34860和Glyma10g07601的表达均不同程度上调，其中，Glyma10g07601基因在大豆品种安达农家、黑大豆、绥农14和黑农40达显著水平（P＜0.05），分别增加1.37、1.22、1.98倍和1.67倍；Glyma03g34860基因在大豆品种垦丰16、绥农14达显著水平（P＜0.05），分别增加1.62倍和1.65倍，因此推测他们可能通过使基因组DNA脱甲基化而参与大豆连作综合逆境胁迫的响应。 |
| 英文摘要: |
| The soybean genome database phytozome v12.1.6 was used to obtain the CDS sequences of soybean DNA demethylase\|related genes Glyma03g34860 and Glyma10g07601. Using soybean leaf RNA as template, 5 226 bp Glyma03g34860 and 6 045 bp Glyma10g07601 were successfully cloned by RT-PCR method. The interacting proteins predicted by using STRING online software were completely the same. There were 8 main interaction proteins that include one AP site lyase and two RNA polymerase subunits. The qRT-PCR results showed that the expressions of Glyma03g34860 and Glyma10g07601 were both up\|regulated with different degree under continuous cropping stress. Of these, the level of Glyma10g07601 was significantly increased by 1.37, 1.22, 1.98 times, and 1.67 times in Andanongjia, Heidadou, Suinong 14, and heinong 40 varieties,respectively (P<0.05), while the level of Glyma03g34860 was significantly increased by 1.62 times and 1.65 times in Kenfeng 16 and Suinong 14 varieties, respectively (P<0.05), suggesting that they participated in the response on continuous cropping comprehensive stress through demethylating genomic DNA in soybean. |
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