| 陆晏天,陈奋奇,白明兴,庄泽龙,丁永福,彭云玲.外源赤霉素对不同耐深播性玉米自交系的深播缓解效应[J].干旱地区农业研究,2021,39(2):37~46 |
| 外源赤霉素对不同耐深播性玉米自交系的深播缓解效应 |
| Deep sowing alleviating effect of exogenous Gibberellin on maize inbred lines with different depth sowing tolerance |
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| DOI：10.7606/j.issn.1000-7601.2021.02.06 |
| 中文关键词: 玉米 深播胁迫 外源赤霉素 缓解效应 |
| 英文关键词:maize deep sowing stress exogenous Gibberellin alleviating effect |
| 基金项目:甘肃省重大科技专项（17ZD2NA016）；甘肃农业大学伏羲“青年英才”项目（GAUFX-02Y09） |
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| 中文摘要: |
| 为探讨不同浓度的外源赤霉素（GA）对深播胁迫下玉米种子萌发、幼苗生长的影响，综合评价GA对深播的缓解效果，以2份深播敏感自交系（‘吉853’和‘自330’）和2份耐深播自交系（‘N192’和‘Mo17’）为试材，于其苗期在2种不同深播（15、20 cm）胁迫下施加4种不同浓度外源GA。结果表明：（1）深播胁迫下出苗率、苗长、根长显著降低，中胚轴长、胚芽鞘长、中胚轴和胚芽鞘长之和，胚芽鞘中的SOD酶活性，中胚轴、胚芽鞘中POD、CAT酶活性及木质素含量显著升高，其变化率（RC）介于-75.86%～100.19%。施加外源GA后幼苗各性状增辐达4.25%～389.06%。（2）用2份深播敏感自交系对外源GA的最佳浓度进行筛选，15 cm和20 cm深播胁迫下分别施加4.0 mg·L-1和6.0 mg·L-1外源GA的缓解效果最佳，隶属函数值分别为0.602和0.598。（3）最佳浓度下比较分析2种耐深播性不同玉米自交系的生长参数和生理特性，表明外源GA可缓解深播对玉米幼苗的胁迫，外源GA对耐深播性不同玉米自交系的敏感程度不同，表现为吉853>自330>Mo17>N192。（4）利用主成分与热图分析玉米萌发期深播性鉴定的三级评价指标，出苗率、中胚轴长、中胚轴和胚芽鞘长之和是主要的评价指标，中胚轴中SOD、POD、CAT活性及中胚轴、胚芽鞘中木质素含量可作为二级评价指标，根数和苗长可作为三级评价指标。 |
| 英文摘要: |
| To explore the different concentrations of exogenous Gibberellin (GA) on the germination and seedling traits of maize seeds under deep sowing stress, the mitigation effect of GA on deep sowing was comprehensively evaluated. In this experiment, two deep-seeding sensitive inbred lines and two deep-seeding resistant inbred lines were used as test materials. At seedling stage, four exogenous GA with different concentrations were added under two kinds of deep-seeding stress. The results showed that: (1) The seeding emergence rate, seedling length and root length were significantly reduced under deep sowing stress, the length of mesocotyl and coleoptile, the sum length of mesocotyl and coleoptile, SOD activity in coleoptile, POD, CAT activity, and lignin content in mesocotyl and coleoptile increased significantly. The RC was between -75.86%~100.19%. After the addition of exogenous GA, all seedling traits increased significantly by 4.25%~389.06%, respectively. (2) Screen the optimal concentration of exogenous GA with two deep-seeding sensitive inbred lines, the relief effect of applying 4.0 mg·L-1 and 6.0 mg·L-1 exogenous GA under 15 cm and 20 cm deep sowing stress were optimal. The membership function values were 0.602 and 0.598. (3) At the optimal concentration, comparative analysis of the growth parameters and physiological characteristics of two maize inbred lines with different sowing tolerances showed that exogenous GA alleviated the stress damage of maize seedlings by deep sowing and restore physiological and biochemical metabolic steady state. However, the exogenous GA had different sensitivity to maize inbred lines with different tolerance to deep sowing, Ji853 > Zi330 > Mo17 > N192. (4) In order to further accurately evaluate the tolerance of different varieties of maize to deep sowing, the main components and heat map analysis were used to obtain the three-level evaluation indicators for the identification of deep sowing at the germination stage of maize: emergence rate, mesocotyl axis length, mesocotyl axis, and the sum of sheaths was the main evaluation index. The SOD， POD， CAT activity in the mesocotyl, and the lignin content in the mesocotyl and coleoptile can be used as secondary evaluation indicators, and the number of roots and seedling length can be used as third-level evaluation indicators. |
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