| 李学才,常瑜,白静,武军艳,李博文,赵玉红,孙柏林,王万鹏,唐德耀,范小龙,朱文英,马骊,孙万仓.外源ABA和钨酸钠对干旱胁迫下白菜型冬油菜ZEP表达的影响[J].干旱地区农业研究,2021,39(2):47~54 |
| 外源ABA和钨酸钠对干旱胁迫下白菜型冬油菜ZEP表达的影响 |
| Effects of exogenous ABA and sodium tungstate on ZEP expression in winter Brassica rapa under drought stress |
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| DOI：10.7606/j.issn.1000-7601.2021.02.07 |
| 中文关键词: 白菜型冬油菜 干旱胁迫 外源ABA 外源钨酸钠 ZEP基因 克隆 表达 |
| 英文关键词:winter Brassica rapa drought stress exogenous ABA exogenous sodium tungstate ZEP gene clone expression |
| 基金项目:甘肃农业大学干旱生境作物学重点实验室开放基金课题(GSCS-2020-06)；公招博士科研启动基金(GAU-KYQD-2019-17)；国家现代农业产业技术体系(CARS-12) |
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| 中文摘要: |
| 为研究白菜型冬油菜玉米黄质环氧酶（ZEP）基因在干旱胁迫下响应外源ABA的分子机制，本研究选择白菜型冬油菜陇油7号和天油4号为试验材料，分别喷施蒸馏水、ABA溶液、钨酸钠溶液、ABA+钨酸钠溶液200 mL后置于植物培养箱干旱处理7 d。采用RT-PCR和qPCR等技术进行ZEP基因克隆和表达分析，结果表明: 该基因包含一个长度为2 013 bp的ORF，编码含670个氨基酸残基的蛋白质，理论等电点为6.16，属于亲水性蛋白。该基因与拟南芥、甘蓝型油菜的同源性分别为89.25%和99.40%。实时荧光定量分析表明，干旱胁迫可诱导白菜型冬油菜ZEP基因上调表达，外源喷施ABA后陇油7号叶片ZEP基因相对表达量较CK升高66.64%，喷施外源ABA合成抑制剂钨酸钠后陇油7号叶片ZEP基因表达量较CK降低12.74%，而同时喷施外源ABA和钨酸钠后，干旱胁迫下陇油7号、天油4号叶片ZEP基因表达量较CK分别增加了3.3倍和2.28倍，陇油7号叶片中的ZEP基因表达量较根系高21.61%，表明ZEP基因在白菜型冬油菜抗旱中可能发挥一定作用。 |
| 英文摘要: |
| In order to study the molecular mechanism of zeaxanthin epoxidase (ZEP) gene in response to exogenous ABA under drought stress, in this study, winter rapeseed Longyou7 and Tianyou4 were selected as test materials, and 200 mL of distilled water, ABA solution, sodium tungstate solution, and ABA + sodium tungstate were sprayed, respectively, and placed in a plant incubator for 7 days for drought stress. RT-PCR and qPCR were used to clone and analyze the expression of ZEP gene, the bioinformatics analysis showed that the gene contained an ORF of 2013 bp, encoding a protein with 670 amino acid residues, and the theoretical isoelectric point was 6.16, belonging to hydrophilic protein. The homology of the gene with Arabidopsis thaliana and Brassica napus was 89.25% and 99.40%, respectively. The real-time fluorescence quantitative analysis showed that drought stress could induce the up regulation of ZEP gene expression in winter rapeseed. The relative expression of ZEP gene in leaves of Longyou7 increased by 66.64% compared with the CK after spraying exogenous ABA, and the expression of ZEP gene in leaves of Longyou7 decreased by 12.74% compared with the CK after spraying exogenous ABA synthesis inhibitor sodium tungstate. After spraying exogenous ABA and sodium tungstate at the same time, the expression of ZEP gene in leaves of Longyou7 and Tianyou4 increased by 3.3 and 2.28 times, respectively, compared with the CK under drought stress, and the expression of ZEP gene in leaves of Longyou 7 was 21.61% higher than that in roots, indicating that ZEP gene may play a role in drought resistance of winter Brassica rapa. |
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