| 陈明媛,张淑英,王梦柯,吴莺.外源硫化氢对NaCl胁迫下棉花幼苗抗逆生理特性的影响[J].干旱地区农业研究,2021,39(2):55~61 |
| 外源硫化氢对NaCl胁迫下棉花幼苗抗逆生理特性的影响 |
| Effects of exogenous hydrogen sulfide on stress resistance physiological characteristics of cotton seedlings under NaCl stress |
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| DOI：10.7606/j.issn.1000-7601.2021.02.08 |
| 中文关键词: 外源硫化氢 NaCl胁迫 棉花幼苗 光合特性 抗氧化酶活性 |
| 英文关键词:exogenous hydrogen sulfide NaCl stress cotton seedlings photosynthetic characteristic activity of antioxidant enzymes |
| 基金项目:国家自然科学基金 (41461064); 石河子大学高层次人才科研启动项目 (RCSX201720) |
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| 中文摘要: |
| 为探讨外源H2S对盐胁迫下棉花幼苗生理响应的调节机制，以新陆早48号棉花为试验材料，采用蛭石盆栽处理，通过营养液培养法，设盐(NaCl，0、100、200 mmol·L-1)和硫化氢(使用硫化氢供体NaHS，0、20、50、100、500 μmol·L-1)两因素交互，共11个处理，研究外源H2S对盐胁迫下棉花幼苗生长、叶绿素含量、叶绿素荧光参数、光合气体参数及抗氧化酶活性的影响。结果表明，相同NaCl不同H2S浓度处理下棉花幼苗的生物量均有不同程度增长，如T2、T3、T4、T5与T1相比，棉苗地上部鲜质量分别增加了35.34%、28.79%、9.31%、19.66%。盐处理后，叶绿素荧光及光合气体参数等测定值均降低，当NaCl浓度为200 mmol·L-1时，qP、qN、Y(NPQ)和Y(NO)分别降低了3.52%、6.14%、17.06%、17.65%; 净光合速率、气孔导度、胞间二氧化碳浓度、蒸腾速率分别降低了18.87%、49.43%、15.1%、18.81%。NaCl浓度为100 mmol·L-1时，与T1相比，T4的叶绿素a含量、叶绿素b含量、叶绿素总含量分别提高了13.04%、28.09%、10%；NaCl浓度为200 mmol·L-1时，与K1相比, K4的叶绿素a含量、叶绿素b含量、叶绿素总含量分别提高了14.18%、52.05%、15.23%。随着盐浓度的升高棉花幼苗抗氧化酶活性受到抑制，外施H2S后其抑制效应得到缓解，如K4与K1相比，其过氧化物酶(POD)活性、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性分别提高了85.02%、28.13%、40.47%，其中以H2S浓度为100 μmol·L-1时效果最佳。 |
| 英文摘要: |
| In order to investigate the regulatory mechanism of exogenous hydrogen sulfide (H2S) on physiological response of cotton seedlings under salt stress, Xinluzao 48 was used as a cotton variety for testing material. The experiment was conducted by a pot culture that roseite was used as the culture pot with nutrient solution. Salt (NaCl, 0, 100 mmol·L-1 and 200 mmol·L-1) and sodium hydrogen sulfide (NaHS, 0, 20, 50, 100 μmol·L-1 and 500 μmol·L-1) were composed to a total of 11 treatments by random combination. The effects of exogenous H2S on the growth, chlorophyll content, chlorophyll fluorescence, photosynthetic gas parameters, and antioxidant enzyme activity of cotton seedlings under salt stress were studied. The results showed that the biomass of cotton seedlings increased in different degrees under the same NaCl and different H2S concentrations, such as T2, T3, T4, and T5 comparing with T1, the above-ground fresh weight of cotton seedlings increased by 35.34%， 28.79%， 9.31%, and 19.66%, respectively. After salt treatment, chlorophyll fluorescence and photosynthetic gas parameters were reduced. When NaCl concentration was 200 mmol·L-1, qP, qN, Y, Y(NPQ), and Y(NO) decreased by 3.52%, 6.14%, 17.06%, and 17.65%, respectively. Net photosynthetic rate, stomatal conductance, intercellular carbon dioxide concentration, and transpiration rate decreased by 18.87%, 49.43%, 15.1%, and 18.81%, respectively. When the concentration of NaCl was 100 mmol·L-1, compared with T1, the content of chlorophyll a, chlorophyll b, and total chlorophyll in T4 increased by 13.04%, 28.09%, and 10%, respectively. When the concentration of NaCl was 200 mmol·L-1, compared with K1, the content of chlorophyll a, chlorophyll b, and total chlorophyll in K4 increased by 14.18%, 52.05%, and 15.23%, respectively. With the increase of salt concentration, the activities of antioxidant enzyme in cotton seedlings were inhibited, and the inhibition effect was alleviated after the application of H2S. For example, compared with K1, the activities of POD, SOD, and CAT in K4 were increased by 85.02%, 28.13%, and 40.47%, respectively. The optimum concentration of H2S was 100 μmol·L-1. |
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