| 黄钰芳,张恩和,张新慧,曹瑞,谢田朋,陈红刚,高素芳.兰州百合不同连作年限土壤中化感物质的检测及其自毒效应的研究[J].干旱地区农业研究,2021,39(2):62~68 |
| 兰州百合不同连作年限土壤中化感物质的检测及其自毒效应的研究 |
| Identification of soil allelochemicals and autotoxicity of Lilium davidii var. unicolor salisb in different continuous cropping years |
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| DOI：10.7606/j.issn.1000-7601.2021.02.09 |
| 中文关键词: 兰州百合 根际土壤 连作 化感物质 自毒作用 |
| 英文关键词:Lilium davidii var. unicolor salisb rhizosphere soil continuous cropping allelochemicals autotoxicity |
| 基金项目:国家自然科学基金（31560380）；甘肃省高等学校创新能力提升项目（2019A-072） |
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| 中文摘要: |
| 为探讨兰州百合（Lilium davidii var. unicolor）连作障碍的可能机理，收集了兰州百合正茬、连作2 a及连作4 a不同生育期的根际土壤，采用GC-MS技术对不同土壤中的化感物质进行了鉴定，并通过生物试验验证了典型自毒物质对苯二甲酸二辛酯（D）和抗氧剂2246（A）对兰州百合幼苗生长的影响。结果显示：从生长旺盛期兰州百合正茬、连作2 a及4 a土壤中分别鉴定出11、15和19种化合物，收获期鉴定出9、15及17种成分，主要包括：醇类、醛类、酯类、酚酸类及少量萜类化合物。其中，对苯二甲酸二辛酯（D）在不同生育期连作土壤中均存在，抗氧剂2246（A）除收获期正茬土壤外在其余土壤中也均存在，且二者相对含量均较高。生物试验结果表明，0.2 mg·mL-1时， D0.2对兰州百合幼苗生长的综合化感效应（SE）为-0.003，具有抑制效应， A0.2的SE值为0.131，具有促进作用；5.0 mg·mL-1浓度下， D5和A5均能抑制兰州百合幼苗的生长，SE值分别为-0.489和-0.040，对苯二甲酸二辛酯的抑制效应更强；共施条件下，D0.2A5和D5A5混合液仍能抑制兰州百合幼苗生长，但未体现出2种成分单施时的抑制协同效应；D5A0.2混合液表现出抑制作用，D0.2A0.2表现出促进作用，体现了2种成分单施时相反作用的叠加效应。 |
| 英文摘要: |
| The rhizosphere soils were collected under field conditions in the stubble, continuous cropping for 2 years and continuous cropping for 4 years of Lanzhou lily (Lilium davidii var. unicolor salisb) to explore the possible obstacle mechanisms of continuous cropping of Lanzhou lily. The rhizosphere soils were collected at the vigorous growth stage and harvest stage, respectively. The chemical composition in different soils were determined by a GC-MS, and the effect of typical autotoxic substances on the growth of Lanzhou lily seedlings were tested by biological experiments. The results showed that eleven, fifteen, and nineteen compounds were identified in the rhizosphere soil from the first crop, continuous cropping for 2 years, and continuous cropping for 4 years of Lanzhou lily at the vigorous growth stage, and nine, fifteen, and seventeen compounds were identified in the rhizosphere soil from the above three treatments at harvest stage, including alcohols, aldehydes, esters, phenolic acids, and small amounts of terpenoids. Among them, dioctyl terephthalate (D) was present in continuous cropping soil at different growth stages, and antioxidant 2246 (A) was also present in other soils except normal cropping soil at harvest stage, and the relative contents of both were higher. The results of biological experiments showed that at 0.2 mg·mL-1, the synthesis effect of dioctyl terephthalate (D0.2) on the growth of Lanzhou lily seedlings was -0.003, showing inhibitory effect. The SE value of antioxidant 2246 (A0.2) was 0.131, showing promoting effect. At the concentration of 5.0 mg·mL-1, both dioctyl terephthalate (D5) and antioxidant 2246 (A5) could inhibit the growth of Lanzhou lily seedlings, with SE values of -0.489 and -0.040, respectively. The inhibition effect of dioctyl terephthalate was stronger. A cross mixture of 0.2 mg·mL-1 and 5.0 mg·mL-1 dioctyl terephthalate and antioxidant 2246 was prepared and the effect of the two-component mixture on Lanzhou lily seedlings was studied. The results showed that the mixture of D0.2A5 and D5A5 still inhibited the growth of Lanzhou lily seedlings under the combined conditions, but did not reflect the synergistic effect of inhibition when the two components were applied alone. The D5A0.2 mixture showed an inhibitory effect, and D0.2A0.2 showed a promoting effect, reflecting the superposition effect of the opposite effect of the two components alone. |
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