| 廉华,陈玉蓉,李梅,梁枭,马光恕.木霉分生孢子和厚垣孢子对黄瓜幼苗生理特性及枯萎病防效的影响[J].干旱地区农业研究,2021,39(2):227~235 |
| 木霉分生孢子和厚垣孢子对黄瓜幼苗生理特性及枯萎病防效的影响 |
| The effect of Trichoderma conidia and chlamydospores on physiological characteristics of cucumber seedlings and control effect against Fusarium wilt |
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| DOI：10.7606/j.issn.1000-7601.2021.02.29 |
| 中文关键词: 木霉 孢子 黄瓜幼苗 生理特性 枯萎病 防治效果 |
| 英文关键词:Trichoderma spore cucumber seedling physiological characteristics fusarium wilt control efficacy |
| 基金项目:国家重点研发计划项目（2018YFD0201202） |
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| 中文摘要: |
| 采用前期筛选出的对黄瓜枯萎病菌有较好拮抗作用的3株木霉菌，即哈茨木霉Trichoderma harzianum 809、拟康氏木霉Trichoderma pseudokoningii 886和棘孢木霉Trichoderma asperellum 525，利用盆栽试验，测定了木霉菌孢子不同类型施用对黄瓜幼苗生长、生理特性及枯萎病防效的影响。结果表明：哈茨木霉809、拟康氏木霉886、棘孢木霉525分生孢子和厚垣孢子对黄瓜枯萎病的盆栽防效均在66.81%以上，且以拟康氏木霉886厚垣孢子防效最高，达到81.46%。当黄瓜幼苗长至三叶一心时，3株木霉菌分生孢子和厚垣孢子处理的黄瓜幼苗株高、茎粗、根体积、叶面积、全株鲜质量均比CK显著上升，以厚垣孢子886的促进效果最显著，其株高、茎粗、根体积、叶面积、全株鲜质量分别较CK增加了85.33%、108.43%、235.29%、144.38%、272.32%。同时，3株木霉菌分生孢子和厚垣孢子处理的黄瓜幼苗叶绿素含量、根系活力、叶片可溶性糖含量、叶片可溶性蛋白含量也均比CK显著上升，厚垣孢子886的促进效果仍最显著，其叶绿素含量、根系活力、叶片可溶性糖含量、叶片可溶性蛋白含量分别较CK增加了97.47%、86.05%、172.49%、201.91%。 |
| 英文摘要: |
| The effect of different types of spores of three Trichoderma strains on the seedling growth, physiological characteristics, and control effects against fusarium wilt of cucumber through a pot culture were evaluated. The three Trichoderma strains were Trichoderma harzianum 809, Trichoderma pseudokoningii 886, and Trichoderma asperellum 525 with anti-fusarium oxysporum capability. The results showed that the control effects of conidia and chlamydospore of three Trichoderma strains against cucumber fusarium wilt were all above 66.81%, and chlamydospore of T. asperellum 886 was the most effective one, with disease control efficacy of 81.46%. Compared with CK, plant height, stem diameter, root volume, leaf area, and fresh weight of the whole plant of cucumber seedlings significantly improved under conidia and chlamydospores of three Trichoderma strains at three leaves and one heart period, chlamydospore of T. asperellum 886 was the most effective one, and plant height, stem diameter, root volume, leaf area, and fresh quality of the whole plant of cucumber seedlings increased by 85.33%,108.43%, 235.29%, 144.38% and 272.32%, respectively, compared with CK. At the same time, chlorophyll content, root activity, leaf soluble sugar content, and leaf soluble protein content of cucumber seedlings significantly improved under conidia and chlamydospores of three Trichoderma strains at three leaves and one heart period compared with that of CK, and chlamydospore of T. asperellum 886 was the most effective one, and chlorophyll content, root activity, leaf soluble sugar content, and leaf soluble protein content of cucumber seedlings increased by 97.47%, 86.05%, 172.49%, and 201.91%, respectively, compared with that of CK. |
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