钟源,赵小强.187份玉米自交系抗旱性评价及SSR标记关联分析[J].干旱地区农业研究,2021,(3):1~8
187份玉米自交系抗旱性评价及SSR标记关联分析
Evaluation on drought resistance of 187 maize inbred lines and association analysis with SSR markers
  
DOI:10.7606/j.issn.1000-7601.2021.03.01
中文关键词:  玉米自交系  干旱  农艺性状  SSR标记  关联分析
英文关键词:maize inbred line  drought  agronomic trait  SSR marker  association analysis
基金项目:甘肃农业大学科技创新基金(GAU-KYQD-2018-19; GAU-KYQD-2018-12);国家自然科学基金项目(32060486);甘肃农业大学甘肃省干旱生境作物学重点实验室开放基金(GSCS-2019-8; GSCS-2020-5);甘肃省高等学校创新能力提升项目(2019A-052; 2019A-054);兰州青绿仪器技术有限公司横向项目(WT20191025)
作者单位
钟源 Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
赵小强 Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
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中文摘要:
      在正常供水 (CK)及干旱胁迫处理(S)下测定187份玉米自交系的株高、穗位高、穗位比、雄穗分枝数、散粉-吐丝时间间隔(ASI)及雌穗个数6个农艺性状,综合评价相应自交系的抗旱性强弱;采用145对SSR标记通过关联分析检测不同水旱环境下与玉米这6个农艺性状显著关联的分子标记。结果表明:干旱胁迫下187份玉米自交系农艺性状除ASI显著增大外,其余5个农艺性状均显著降低,且干旱胁迫下这些性状的变化幅度介于-72.73%~11.96%;以株高、穗位高、穗位比、雄穗分枝数、散粉-吐丝时间间隔(ASI)及雌穗个数6个性状的抗旱系数作为玉米抗旱性评价指标,并采用隶属函数法筛选出强抗旱自交系45份、中等抗旱自交系74份、旱敏感自交系68份。用一般线性模型(GLM)在不同水旱环境下共检测到了13个SSR位点与这6个性状在P<0.01水平显著关联,其主要位于第1、2、3、4、5、7、8及9号染色体上,单个位点可解释表型变异的2.15%~17.13%,大约85.19%的SSR位点可在干旱胁迫环境下检测到;其中bnlg149 (Bin 1.01)、phi053 (Bin 3.05)、umc1621a (Bin 4.06)、umc1155 (Bin 5.05)、umc1072 (Bin 5.07)及umc1120 (Bin 9.04)等6个SSR与不同水旱环境下的多个农艺性状同时连锁,表现出明显的一因多效现象。
英文摘要:
      The agronomic traits, namely plant height (PH), ear height (EH), ear height\|to\|plant height ratio (EHPH), tassel branch number (TBN), anthesis\|silking interval (ASI), and ear number per plant (EN) were closely associated with drought resistance in maize (Zea mays L), elucidating the genetic basis of these agronomic traits under different watering environments can provide theoretical support for molecular breeding with drought resistance in maize. Six agronomic traits in 187 maize inbred lines were measured under different watering environments, and the drought resistance of corresponding inbred lines were comprehensively evaluated; and then the association between molecular markers and six agronomic traits under different watering environments were detected with 145 SSR markers in this study. The results showed that: (1) The ASI significantly increased in 187 inbred lines under drought stress, however, other five agronomic traits significantly decreased, and the degrees of change on these traits under drought stress were -72.73%~11.96%. (2) The drought resistance coefficient of the above six traits were used as the evaluation index of drought resistance in maize, and then 45 strong drought resistant inbred lines, 74 medium drought resistant inbred lines, and 68 drought sensitive inbred lines were screened out by the membership function method. (3) Total 13 SSR markers were found to associated with (P<0.01) these six traits under different watering environments by general linear model (GLM) program, the corresponding significant SSR were distributed on chromosomes 1, 2, 3, 4, 5, 7, 8, and 9, and the phenotypic variance explained were 2.15%~17.13%, then approximately 85.19% SSR were detected under drought stress; The bnlg149 (Bin 1.01), phi053 (Bin 3.05), umc1621a (Bin 4.06), umc1155 (Bin 5.05), umc1072 (Bin 5.07), and umc1120 (Bin 9.04) were simultaneous associated with multiple agronomic traits under different watering environments, which significantly showed “a pleiotropic effect”. These results can provide useful information for selection and improvement of drought resistant inbred lines, and marker\|assisted selection (MAS) breeding with drought resistance in maize.
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